Detailed information of component protein


Summary

Component ID T6CP004920
Component protein type IglH
T6SS ID (Type) T6SS00158 experimental (Type ii)
Strain Francisella tularensis subsp. novicida U112
Replicon chromosome
Sequence Protein sequence (477 a.a.); Nucleotide sequence (1431 bp)
Reference
[1] de Bruin OM, Duplantis BN, Ludu JS, et al. The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion. Microbiology (Reading). 2011 Dec;157(Pt 12):3483-3491. doi: 10.1099/mic.0.052308-0. Epub 2011 Oct 6. PMID: 21980115
[2] Robb CS, Nano FE, Boraston AB. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of intracellular growth locus E (IglE) protein from Francisella tularensis subsp. novicida. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1596-8. doi: 10.1107/S1744309110034378. Epub 2010 Nov 25. PMID: 21139203
[3] Nano FE, Schmerk C. The Francisella pathogenicity island. Ann N Y Acad Sci. 2007 Jun;1105:122-37. Epub 2007 Mar 29. PMID: 17395722
[4] Bröms JE, Meyer L, Sun K, et al. Unique substrates secreted by the type VI secretion system of Francisella tularensis during intramacrophage infection. PLoS One. 2012;7(11):e50473. doi: 10.1371/journal.pone.0050473. Epub 2012 Nov 20. PMID: 23185631
[5] Barker JR, Chong A, Wehrly TD, et al. The Francisella tularensis pathogenicity island encodes a secretion system that is required for phagosome escape and virulence. Mol Microbiol. 2009 Dec;74(6):1459-70. PMID: 20054881
[6] Hare RF, Hueffer K. Francisella novicida pathogenicity island encoded proteins were secreted during infection of macrophage-like cells. PLoS One. 2014 Aug 26;9(8):e105773. doi: 10.1371/journal.pone.0105773. eCollection 2014. PMID: 25158041
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) FTN_RS06735
Coordinate (Strand) 1387186..1388616 (+)
NCBI ID WP_003040123.1
RefSeq NC_008601
Uniprot ID A0A6I4RQJ5_FRATU, A0Q7H6_FRATN
KEGG ID ftn:FTN_1315, ftx:AW25_689
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-476 MDEKRKDLSTNDASILKKTFDKLSNDIRDDFDKRIFDYSNALLFKYYSNLYYFVPKSCLLEINDKGHNGYYIPPQEYFFIEDKRTGKTLTYTTKSENIILPIRDITTIKQSDTSINISLEFANNFDYDYVTIWLDPTLCKEDPSFATYIFNQVLEDNGSAKIKFSNHSYTTKNVQIEPIKFQLKPTEKLVLNIHSPSLLYGFNIKIKDLFKYKNNDLNKIDFFLEVKTENYNDEKLSSLFRVNLIPVFNSFDDYSSTSFASMNLSESKLKHHQKDDAQAIEVLSVYENNKQCDFNSFLFVGQNEYYFNRSTQSLNYSSVLPQFSNKIIGTKIHTYTTWTQITEISDLIEINSNAIASISCNISPIVINKALNNYNSNAKEMFNIIDMIISKNMYSKTTFLAIAKLLKINTNDLTLLSMLLQDVEMDSTINELILTLSDKYNPKYKHFLNFHIGIICKFINQNTFSFIKKIVLNNSI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9886 - -


  Protein sequence: 477 a.a.    

>T6CP004920 NC_008601:1387186-1388616 [Francisella tularensis subsp. novicida U112] [IglH]
MDEKRKDLSTNDASILKKTFDKLSNDIRDDFDKRIFDYSNALLFKYYSNLYYFVPKSCLLEINDKGHNGYYIPPQEYFFI
EDKRTGKTLTYTTKSENIILPIRDITTIKQSDTSINISLEFANNFDYDYVTIWLDPTLCKEDPSFATYIFNQVLEDNGSA
KIKFSNHSYTTKNVQIEPIKFQLKPTEKLVLNIHSPSLLYGFNIKIKDLFKYKNNDLNKIDFFLEVKTENYNDEKLSSLF
RVNLIPVFNSFDDYSSTSFASMNLSESKLKHHQKDDAQAIEVLSVYENNKQCDFNSFLFVGQNEYYFNRSTQSLNYSSVL
PQFSNKIIGTKIHTYTTWTQITEISDLIEINSNAIASISCNISPIVINKALNNYNSNAKEMFNIIDMIISKNMYSKTTFL
AIAKLLKINTNDLTLLSMLLQDVEMDSTINELILTLSDKYNPKYKHFLNFHIGIICKFINQNTFSFIKKIVLNNSI*

  Nucleotide sequence: 1431 bp    

>T6CP004920 NC_008601:1387186-1388616 [Francisella tularensis subsp. novicida U112] [IglH]
ATGGATGAAAAAAGAAAAGATTTAAGTACGAATGATGCTAGCATCCTAAAAAAAACTTTTGACAAACTAAGTAATGATAT
AAGAGATGATTTTGATAAGAGAATTTTTGATTATAGTAATGCTCTTTTATTCAAGTATTATAGTAACCTTTATTACTTCG
TTCCTAAATCATGCTTATTAGAAATAAATGATAAAGGACACAATGGTTACTATATTCCGCCTCAGGAATATTTTTTCATA
GAAGACAAGAGAACAGGTAAAACTCTAACATATACGACAAAGTCAGAAAATATAATACTCCCTATTAGAGATATCACAAC
CATAAAACAAAGTGATACATCAATAAATATATCATTGGAATTTGCAAATAACTTTGACTATGATTATGTTACTATCTGGC
TAGATCCAACTTTATGTAAAGAAGATCCTAGCTTTGCCACATACATATTTAACCAAGTATTAGAAGATAATGGCTCAGCA
AAGATTAAATTTTCAAATCATAGTTATACAACAAAAAATGTACAAATAGAACCAATTAAGTTCCAATTAAAACCAACAGA
AAAGTTAGTTCTCAATATCCATTCTCCTAGCCTTTTATATGGTTTTAACATAAAGATCAAAGACTTATTTAAATACAAAA
ATAATGACCTCAACAAAATTGACTTTTTTTTAGAAGTTAAAACTGAAAACTATAATGACGAAAAATTATCATCTCTATTT
AGAGTTAATTTAATACCTGTGTTTAATAGTTTTGATGATTACTCAAGCACCTCATTTGCTTCGATGAATCTCTCAGAGAG
TAAACTAAAACATCATCAAAAAGATGATGCTCAAGCAATAGAAGTATTATCTGTTTATGAGAATAATAAACAATGTGATT
TTAATAGTTTTCTTTTTGTAGGACAGAATGAGTATTATTTTAATCGCAGCACACAATCTTTAAATTATAGTAGCGTACTC
CCTCAATTTAGTAATAAAATAATAGGAACAAAAATACATACATATACAACCTGGACACAGATAACTGAGATTTCTGATCT
AATTGAAATTAACTCAAATGCAATAGCATCCATAAGCTGTAATATTTCTCCAATAGTTATTAACAAAGCACTAAACAACT
ACAATAGCAATGCTAAAGAAATGTTTAATATAATAGATATGATCATCTCTAAGAATATGTATTCAAAAACAACATTCCTA
GCTATAGCAAAGCTTTTAAAGATTAATACTAATGACTTAACTTTACTATCAATGCTCCTACAAGATGTTGAGATGGACTC
TACTATAAATGAGCTAATTTTAACCTTAAGTGATAAATATAACCCAAAATATAAACATTTCTTAAATTTTCATATTGGTA
TTATATGCAAATTTATAAATCAAAACACCTTTAGCTTTATTAAAAAGATTGTTTTAAATAACTCTATATAA