Detailed information of regulator


Summary

Regulator ID REG0003 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-HK
KEGG pathway (determined by BlastKOALA) Two-component system (map02020); Biofilm formation - Escherichia coli (map02026)
Related T6SS T6SS01264 experimental (Type i3)
Strain Serratia marcescens strain RM66262
Replicon chromosome
Sequence Protein sequence (954 a.a.); Nucleotide sequence (2862 bp)
Description two-component system sensor histidine kinase RcsC; an rcsC background resulted in an increase in Hcp secretion levels.
Reference
External database links

Locus tag (Gene) RT90_01105 (rcsC)
Coordinates (Strand) 232638..235499 (+)
NCBI ID 735006326
GenBank JWLO01000001
Uniprot ID A0A2V4G8G1_SERMA
KEGG ID -
KEGG pathway (determined by BlastKOALA) map02020; map02026
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
RT90_01115 (rcsB) ↑ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
RcsC PF09456.12 1.1e-31 711..801
Response_reg PF00072.26 4e-30 831..939
HATPase_c PF02518.28 1.2e-27 583..692
HisKA PF00512.27 4.7e-16 471..535
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-19 MKYLASFRTTLKISRYLFR
Transmembrane helix 20-42 VLAILLWSLGALLTTFYILNILH
Inside 43-316 QKESDIRQEYNLNFDQAQGYIRHSADIIRDIKYMAENRLNGSVSGLDMFSGVIPGKGSPPQFFPLYPESNCALSTTYRSSLDSLSGLIQYWKENFVAAYDLNRVFFIGGDSLCMAEFGGGNASANRENMLKLLHERILKYRNAKNLDKDNNLYWISPSAQRPDVGYLYVLTPLYIGNKLEALLGIEQTVRLEDFVTAGNLPIGVTLLDENNEPVLRLADGERYAAALNSYPEEHAYFGYVDNYRDLILKKALPPSSLSIVYALPVKSVVERFKM
Transmembrane helix 317-339 LILNALLLNLLSAIVLFTLAWLF
Outside 340-953 ERKMFLPAEDNAFRLEEHEQFNRKIVASAPVGICILRISDGTNILSNELAHNYINLLTHEDRDRITRIICEQQANFVDVMTSNNNNLQISFVHSRYRNEEVAICVLVDVSARVKMEESLQEMAAAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKALPQGVDRLVNAMNNSSGLLLKIISDILDFSKIESEQLKIEPREFSCLEVITHIAGNYLPLVVKKRLGLYCFIEQNVPERIFGDPVRLQQVLSNLVNNAIKFTDTGCIVLQVCTRGSYLEFSVRDTGVGIPEKEISRLFDPFFQVGTGVQRHFQGTGLGLAICEKLVNLMDGDVSVESEPGLGSLFSIRIPLFNAQFPIPQASDTWQGRRLWLDIRNQRLESYLMAILGGYGADIQRYDGQETAAGEVLLSDHPLMLDAPLLAQIQFSTEHIGPSQETRPGYWMHSTSTPRETLTLLNRLFGVGSGAAEALVQLPVPAKASAADNGDIHLLVVDDHPINRRLLSDQLGSLGYQVVTANDGVDALGVLKQHRVDIVLTDVNMPNMDGYRLTQALRQMQFSAPVIGVTANALAEEKQRCLEAGMDNCLSKPVTLETLEQTLAYYSQQVRYSRSEA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9940 - -


  Protein sequence of regulator: 954 a.a.    .

>REG0003 JWLO01000001:232638-235499 [Serratia marcescens]
MKYLASFRTTLKISRYLFRVLAILLWSLGALLTTFYILNILHQKESDIRQEYNLNFDQAQGYIRHSADIIRDIKYMAENR
LNGSVSGLDMFSGVIPGKGSPPQFFPLYPESNCALSTTYRSSLDSLSGLIQYWKENFVAAYDLNRVFFIGGDSLCMAEFG
GGNASANRENMLKLLHERILKYRNAKNLDKDNNLYWISPSAQRPDVGYLYVLTPLYIGNKLEALLGIEQTVRLEDFVTAG
NLPIGVTLLDENNEPVLRLADGERYAAALNSYPEEHAYFGYVDNYRDLILKKALPPSSLSIVYALPVKSVVERFKMLILN
ALLLNLLSAIVLFTLAWLFERKMFLPAEDNAFRLEEHEQFNRKIVASAPVGICILRISDGTNILSNELAHNYINLLTHED
RDRITRIICEQQANFVDVMTSNNNNLQISFVHSRYRNEEVAICVLVDVSARVKMEESLQEMAAAAEQASQSKSMFLATVS
HELRTPLYGIIGNLDLLQTKALPQGVDRLVNAMNNSSGLLLKIISDILDFSKIESEQLKIEPREFSCLEVITHIAGNYLP
LVVKKRLGLYCFIEQNVPERIFGDPVRLQQVLSNLVNNAIKFTDTGCIVLQVCTRGSYLEFSVRDTGVGIPEKEISRLFD
PFFQVGTGVQRHFQGTGLGLAICEKLVNLMDGDVSVESEPGLGSLFSIRIPLFNAQFPIPQASDTWQGRRLWLDIRNQRL
ESYLMAILGGYGADIQRYDGQETAAGEVLLSDHPLMLDAPLLAQIQFSTEHIGPSQETRPGYWMHSTSTPRETLTLLNRL
FGVGSGAAEALVQLPVPAKASAADNGDIHLLVVDDHPINRRLLSDQLGSLGYQVVTANDGVDALGVLKQHRVDIVLTDVN
MPNMDGYRLTQALRQMQFSAPVIGVTANALAEEKQRCLEAGMDNCLSKPVTLETLEQTLAYYSQQVRYSRSEA*

  Nucleotide sequence of regulator: 2862 bp    .

>REG0003 JWLO01000001:232638-235499 [Serratia marcescens]
TTGAAATACTTAGCCTCTTTTCGAACTACGCTGAAGATATCCCGCTATCTGTTCCGGGTACTGGCGATTTTGCTGTGGTC
GTTGGGTGCGCTGCTCACCACTTTTTATATTCTGAATATCCTGCATCAGAAAGAATCGGACATCCGGCAGGAGTACAACC
TCAACTTCGACCAGGCGCAGGGCTACATCCGTCACTCCGCTGACATCATTCGCGACATCAAGTATATGGCGGAAAATCGC
CTCAACGGTTCGGTCAGCGGCCTGGACATGTTCAGCGGCGTGATCCCCGGCAAGGGCAGCCCGCCGCAATTCTTTCCGCT
GTACCCGGAGTCCAACTGCGCGCTGAGCACCACCTATCGCAGTTCGCTCGATTCGCTGAGCGGCCTGATCCAGTATTGGA
AAGAGAACTTCGTGGCGGCCTACGATCTCAACCGGGTGTTCTTCATCGGCGGCGACAGCCTGTGTATGGCGGAATTCGGC
GGCGGCAACGCGAGCGCCAATCGGGAAAACATGCTGAAGTTGCTGCACGAGCGCATTCTGAAATACCGCAACGCCAAGAA
TCTCGATAAAGACAACAACCTGTATTGGATCAGCCCCAGCGCGCAGCGGCCGGACGTCGGTTACCTGTACGTGCTGACGC
CTCTCTATATCGGCAACAAGCTGGAGGCGTTGCTGGGCATCGAGCAAACCGTTCGGCTGGAAGATTTCGTTACCGCCGGC
AACCTGCCGATCGGCGTCACGCTGCTGGATGAGAACAACGAGCCGGTGCTGCGCCTGGCGGATGGCGAACGCTATGCCGC
TGCGCTCAACAGCTACCCCGAAGAGCATGCCTACTTCGGTTATGTCGACAATTACCGCGACCTGATCCTGAAGAAAGCGC
TGCCGCCTTCTTCGCTGAGCATCGTCTATGCGCTGCCGGTGAAAAGCGTGGTGGAACGCTTCAAAATGCTGATCCTCAAC
GCGCTGTTGCTGAACCTGTTGTCGGCCATCGTGCTGTTCACGCTGGCGTGGCTGTTTGAGCGCAAGATGTTCCTGCCGGC
GGAGGACAACGCCTTCCGCCTGGAAGAGCACGAGCAGTTCAACCGCAAGATCGTCGCTTCGGCGCCGGTGGGCATCTGTA
TCCTGCGCATCAGCGACGGCACCAATATTTTGAGTAACGAGCTGGCGCACAACTACATCAACCTGTTGACCCACGAAGAT
CGCGATCGCATAACGCGCATCATTTGCGAACAGCAGGCGAATTTCGTCGATGTGATGACCAGCAACAACAACAACCTGCA
GATCAGCTTTGTCCATTCGCGCTACCGTAACGAAGAGGTGGCGATTTGCGTGTTGGTGGACGTCAGCGCGCGCGTGAAGA
TGGAAGAGTCGCTGCAGGAGATGGCGGCGGCGGCGGAGCAGGCCAGCCAGTCGAAATCGATGTTCCTGGCCACCGTCAGC
CATGAGCTGCGCACGCCGCTGTACGGCATTATCGGCAACCTTGATCTGTTGCAAACCAAGGCGTTGCCGCAAGGGGTCGA
TCGGCTGGTCAATGCGATGAACAACTCTTCCGGCCTGCTGCTGAAAATCATCAGCGACATCCTCGATTTCTCCAAAATCG
AGTCCGAGCAGTTGAAGATTGAACCGCGCGAGTTCTCCTGTCTGGAGGTGATCACCCATATCGCCGGCAACTATTTGCCG
CTGGTGGTCAAGAAACGTCTGGGGCTGTACTGCTTTATCGAACAGAACGTGCCGGAGCGCATCTTCGGCGATCCGGTGCG
GCTGCAGCAGGTGCTCTCCAACCTGGTGAACAACGCCATCAAGTTCACCGATACCGGCTGTATCGTGCTGCAGGTCTGCA
CCCGCGGCAGCTACCTGGAGTTCAGCGTGCGCGATACCGGCGTCGGCATCCCGGAGAAGGAGATTTCGCGCCTGTTCGAT
CCGTTCTTCCAGGTGGGGACCGGCGTGCAGCGTCATTTCCAGGGCACCGGCCTGGGATTGGCGATCTGCGAGAAATTGGT
CAACCTGATGGACGGCGACGTCTCCGTCGAGTCCGAGCCGGGCCTGGGCAGCTTGTTCAGCATTCGCATTCCATTGTTTA
ACGCCCAGTTCCCGATCCCGCAGGCCAGCGATACCTGGCAGGGGCGTCGGCTGTGGCTGGATATCCGCAATCAGCGGCTG
GAAAGCTACCTGATGGCGATCCTGGGCGGCTACGGCGCCGATATCCAGCGCTATGACGGGCAGGAGACCGCCGCGGGAGA
GGTGCTGCTCAGCGATCATCCGCTGATGCTGGATGCGCCGCTGCTGGCTCAGATCCAATTCTCCACCGAGCATATTGGCC
CATCACAGGAAACCCGGCCGGGCTATTGGATGCACAGCACCTCCACGCCGCGTGAAACGCTGACCTTGCTCAACCGGTTG
TTCGGCGTCGGCAGCGGCGCGGCAGAGGCGCTGGTGCAGCTGCCGGTGCCCGCCAAGGCCAGCGCGGCGGACAACGGCGA
TATTCATCTGCTGGTGGTCGACGATCATCCGATCAACCGTCGCCTGTTGTCCGATCAGCTGGGATCGCTGGGATATCAGG
TGGTGACCGCCAACGACGGCGTCGACGCGCTCGGCGTGCTGAAACAGCATCGCGTCGATATTGTGCTGACCGACGTCAAT
ATGCCGAATATGGACGGTTATCGTCTGACGCAGGCGCTGCGGCAGATGCAGTTCAGCGCGCCGGTGATTGGCGTGACGGC
GAACGCGCTGGCGGAAGAGAAGCAGCGCTGCCTCGAGGCGGGTATGGATAATTGCCTGTCGAAACCGGTCACGCTGGAGA
CGCTGGAACAGACGTTGGCTTACTACAGCCAGCAGGTGCGCTACAGCCGCAGCGAGGCTTGA