SecReT6, T6SS database

Detailed information of Recipient susceptibility factor

Summary

ID	RSF000028
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (380 a.a.); Nucleotide sequence (1140 bp)
Response to T6SS attack	protective
Known or putative function	colanic acid synthesis
Description	Deletion of genes involved in colanic acid synthesis (wza and wcaA) regulated by Rcs reduced survival, suggesting that this Rcs-induced EPS protects E. coli against T6SS attacks.
Reference	[1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

External database links

Locus tag (Gene)	BW25113_RS10765 (wza)
Coordinates (Strand)	2129585..2130724 (-)
NCBI ID	446900838
RefSeq	NZ_CP009273
Uniprot ID	A0A1Z3USY0_ECOLX, A0A0E0XXF8_ECO1C, L3C7G6_ECOLX, S1F5Z9_ECOLX, L3Q459_ECOLX, S1HUD5_ECOLX, L4J5Q9_ECOLX, U9XZK9_ECOLX, U9XZ50_ECOLX, V0SLG2_ECOLX, U9ZSC2_ECOLX, V0VGH7_ECOLX, V0YGP8_ECOLX, V0YIK3_ECOLX, U9ZHH3_ECOLX, A0A0E2LJB6_ECOU3, A0A029HXI8_ECOLX, D3GZC4_ECO44, A7ZNN6_ECO24, A0A0E1SUZ5_ECOLX, Q1RA10_ECOUT, A0A0H2Z0D8_ECOK1, B6I8D3_ECOSE, B1LNY1_ECOSM, A0A140N6I2_ECOBD, J7RSK9_ECOLX, D6I9D1_ECOLX, F4NEF2_ECOLX, A0A2S7SP53_ESCFE, A0A0H3EZW4_ECOLW, A0A181WMN6_KLEOX, B7UTA0_ECO27, B7MDL3_ECO45, B7L9T6_ECO55, B7NC96_ECOLU, A0A0H3MFS7_ECO7I, B7MWK3_ECO81, A0A1S9J9N1_SHIBO, A0A0I4U8I3_SHISO, G1UGH7_ECOLX, A0A1X3JEA4_ECOLX, A0A1X3L106_ECOLX, A0A1X3KCY5_ECOLX, F4VGZ1_ECOLX, A0A1X3I5J4_ECOLX, A0A0K9TA06_ECOLX, F4T1D1_ECOLX, A0A1X3LL71_ECOLX, A0A1X3LZ76_ECOLX, A0A1X3IZT9_ECOLX, E9XIF6_ECOLX, E1IJ79_ECOLX, D8E5C2_ECOLX, A0A0E1M3G6_ECOLX, D8A541_ECOMS, D7Y090_ECOM1, E9TE85_ECOLX, WZA_ECOLI, WZA_ECO57, F0JVV6_ESCFE
KEGG ID	ecz:ECS88_2159, ecv:APECO1_1152, ect:ECIAI39_0953, eci:UTI89_C2336, ecw:EcE24377A_2355, ecm:EcSMS35_1000, ecs:ECs2867, esl:O3K_09115, elw:ECW_m2219, ecj:JW2047, ece:Z3227, eco:b2062, ecy:ECSE_2336, ebd:ECBD_1593, elo:EC042_2299, eum:ECUMN_2398, eck:EC55989_2318, ecg:E2348C_2204, ecq:ECED1_2408
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Poly_export	PF02563.18	8.8e-27	81..165
Wza_C	PF18412.3	6e-16	347..376
SLBB	PF10531.11	1.9e-07	173..221

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-379	MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQVDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9986	pos: 20-21. ISG-CT.	MMKSKMKLMPLLVSVTLISG

Protein sequence: 380 a.a. .

                                    >RSF000028 NZ_CP009273:c2130724-2129585 [Escherichia coli BW25113]
MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEI
ANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRL
LYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQL
DMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW*

                                

Nucleotide sequence: 1140 bp .

                                    >RSF000028 NZ_CP009273:c2130724-2129585 [Escherichia coli BW25113]
ATGATGAAATCCAAAATGAAATTGATGCCATTATTGGTGTCAGTAACCTTGATAAGCGGTTGCACAGTACTTCCGGGCAG
CAATATGTCGACGATGGGCAAAGACGTCATCAAACAGCAGGACGCTGATTTCGATCTCGACAAAATGGTGAATGTTTATC
CGCTGACCCCGCGCCTGATTGACCAATTACGCCCACGCCCGAATGTAGCGCGCCCCAATATGACGCTGGAAAGTGAGATC
GCGAATTACCAGTATCGCGTCGGGCCGGGGGACGTTCTTAATGTCACCGTCTGGGATCACCCGGAACTCACCACGCCAGC
CGGTCAGTACCGCAGCTCCAGCGACACCGGCAACTGGGTACAGCCTGACGGCACTATGTTTTACCCGTATATCGGCAAGG
TCCACGTAGTCGGGAAAACGCTCGCTGAAATCCGCAGTGATATTACCGGGCGCTTAGCGACGTACATCGCTGACCCGCAG
GTGGACGTTAATATCGCCGCCTTCCGCTCACAAAAGGCCTATATCTCCGGTCAGGTGAATAAATCCGGTCAACAGGCGAT
CACCAACGTGCCACTGACTATTCTCGACGCCATCAACGCCGCAGGTGGCCTGACCGACACCGCTGACTGGCGCAACGTGG
TGCTAACACACAATGGTCGTGAAGAGCGCATTTCTTTGCAGGCGCTGATGCAAAACGGCGACCTCAACCAGAATCGCCTG
CTTTACCCCGGCGATATTCTCTACGTGCCACGTAATGATGATCTGAAAGTATTTGTGATGGGTGAAGTGAAGAAACAGAG
CACCCTGAAAATGGACTTTAGCGGCATGACCCTGACTGAAGCCCTGGGCAATGCTGAAGGCATCGACATGACCACCTCCA
ACGCCAGCGGCATCTTTGTCATTCGTCCGCTGAAAGGCGAGGGCGGGCGTAACGGCAAGATTGCCAATATCTACCAGCTG
GATATGTCCGATGCCACGTCGCTGGTGATGGCGACAGAATTCCGCCTGCAACCTTATGACGTGGTGTATGTCACCACCGC
CCCGGTTTCCCGCTGGAACCGTCTGATCAATCAGTTGCTGCCAACTATTAGCGGTGTCCGTTACATGACGGATACAGCCA
GCGACATTCATAACTGGTAA

                                