Detailed information of regulator


Summary

Regulator ID REG0249 experimental
Regulator type protein
Regulatory pathway (from literatures) Transcription regulator
KEGG pathway (determined by BlastKOALA) Biofilm formation - Vibrio cholerae (map05111)
Related T6SS T6SS00464 experimental (Type i4b)
Strain Escherichia coli 042
Replicon chromosome
Sequence Protein sequence (99 a.a.); Nucleotide sequence (297 bp)
Description A PaggR::lacZ reporter assay was performed in strains EAEC 042 and the 042fis mutant JS34 and the DNA sequences extending from bases −50 to +114 relative to the predicted transcriptional start are necessary for the FIS-dependent regulation of PaggR.
Reference

External database links

Locus tag (Gene) EC042_RS18855 (FIS)
Coordinates (Strand) 3772795..3773091 (+)
NCBI ID 446385050
RefSeq NC_017626
Uniprot ID G9Z236_9ENTR, A0A1C4E7A1_9ENTR, A0A1C4ELT2_9ENTR, A0A084ZS44_9ENTR, A0A085JQG6_9GAMM, A0A0H3GZF4_KLEOK, FIS_SALPB, A0A0H3FKK0_KLEAK, K8A0R4_9ENTR, A0A0R9P5Z5_SALNE, H5V5N2_ATLHE, A0A090V2S4_PSEVU, A0A0H3GTW9_KLEPH, A0A0E0XX11_ECO1C, H3N3J2_9ENTR, A0A1B1KX64_9GAMM, A0A0K2NXP7_9ENTR, V5Z3Z1_9GAMM, J2DGS4_KLEPN, K4WLE3_ECOLX, R9VRD2_9ENTR, R8WNB1_9ENTR, A0A1J7PVQ8_SALHO, V1H5R3_SALER, A0A1V0L8A6_9ENTR, S1P8L3_ECOLX, S1EM06_ECOLX, L3Q0Y2_ECOLX, L4IZU5_ECOLX, V7IU62_SALET, M7RHP3_SALDU, S5N1A5_SALBN, J0MKZ5_9ENTR, A0A071LZH6_9ENTR, A0A2C9NUP5_SALET, A0A248K4S3_SALBN, A0A0F6RGI8_CITAM, A0A034T6U9_9GAMM, U9XYS8_ECOLX, U9Y9D6_ECOLX, V0STC1_ECOLX, V0A295_ECOLX, V0V4J5_ECOLX, V0X8T4_ECOLX, V0YTZ4_ECOLX, V0AMH8_KLEPN, V0AIF7_ECOLX, A0A0E2L2D0_ECOU3, V3Q058_9ENTR, U2NBQ7_SERFO, S4YLI0_SERPL, A0A1B7I2X7_9ENTR, A0A1B7HKP9_9ENTR, A0A198H0G5_9ENTR, A0A1B7K719_9ENTR, A0A285KUQ0_9ENTR, A0A068Z4R3_9GAMM, W7NVR7_9ENTR, V5AV66_ENTCL, A0A0E1CCC5_KLEPN, W8VDI1_KLEPN, A0A064DK68_CITFR, W0LCY9_9GAMM, A0A080IQE1_ECOLX, A0A0A0FKH7_ECOLX, A0A2K1Q729_9GAMM, A0A1C1EXB8_9ENTR, A0A2S9IBC8_9GAMM, A0A2R4HLF1_SALEN, A0A0L7TGT1_9GAMM, A0A1X7K7N1_9ENTR, A0A1F2M3N8_9ENTR, A0A089URV0_9ENTR, A0A2N0CR08_9ENTR, A0A0U5KX37_9GAMM, A0A0J6MH56_9ENTR, A0A0L0AI95_9ENTR, A0A0J1LQL8_9ENTR, A0A0J1PIK8_9ENTR, A0A0J1MH69_9ENTR, A0A1B7KZZ2_9ENTR, A0A0Q4N8K8_9GAMM, A0A0V9J8A1_9ENTR, A0A167QBZ6_9ENTR, A0A1C7W9Y2_9GAMM, A0A1M5T9P2_9GAMM, A0A1W6B351_9GAMM, A0A1D8K056_9ENTR, A0A2G8EGH3_9GAMM, A0A2I8QLB1_9ENTR, A0A2I8NPJ6_9ENTR, A0A1S8YK87_9GAMM, A0A2T8KZ37_SALET, A0A1V9DD31_9GAMM, A0A0G3QGD9_9ENTR, A0A2J9E2X4_9GAMM, A0A2S4QN10_9ESCH, FIS_ECOL6, A0A2P5GQ13_9ENTR, A0A223U5A7_9ENTR, A0A2W0GJL4_9ENTR, A0A1S8CM17_9GAMM, A0A2S0VGM9_9ENTR, A0A285BAP0_9ENTR, A0A2N5ESA1_9GAMM, A0A2N5DY05_9GAMM, A0A2N5E8Q0_9GAMM, A0A2K9PE86_9ENTR, A0A2I8Q0L4_9ENTR, A0A0F1BE44_9ENTR, A0A2I8SBZ5_9ENTR, A0A2L0THE9_9ENTR, A0A2I8TG74_9ENTR, A0A2P5IRU4_9GAMM, A0A2S4SNW5_9ENTR, A0A0F0YDI5_9ENTR, A0A2S6P7U8_9ESCH, A0A2T3C947_9ENTR, E3DDL6_ERWSE, D3GYQ2_ECO44, A0A0H3NGL5_SALTS, A0A2U3ESK1_9ENTR, A0A0K0HF86_SALBC, A0A0X4EI58_9ENTR, A0A087FW54_KLEVA, FIS_KLEP7, A0A2T2Y1Z5_9ENTR, A0A2S9U7T2_CROSK, A0A1W5DRW2_9GAMM, FIS_CITK8, FIS_CROS8, FIS_SALPA, G8LHA7_9ENTR, FIS_SHIDS, FIS_ECODH, E3PKV2_ECOH1, FIS_SALCH, FIS_ECO24, FIS_ECOHS, A0A2T9Q3L9_SALET, A0A0E1T655_ECOLX, A0A2T8R4U5_SALET, A0A2S4RT24_CITAM, A0A2P8VFS7_9ENTR, FIS_ECOL5, FIS_ECOUT, A0A0A4A9E2_9GAMM, A0A0L0GYG2_9ENTR, FIS_SERP5, FIS_ECOK1, FIS_ECOSE, A0A2T7ASQ2_9ENTR, A0A2T7B0W1_9ENTR, V5U5V9_9ENTR, FIS_SALAR, FIS_SALNS, A0A0J8YBG7_9ENTR, FIS_SALSV, FIS_SALDC, FIS_ECOSM, FIS_ECO5E, FIS_SALA4, FIS_SALHS, FIS_ERWT9, A0A140N7Q6_ECOBD, FIS_SALPC, A0A0H3Q0I0_ECO5C, A0A0F7D192_SERFO, FIS_ECOLC, A0A2R4D4W5_SALET, D0ZFB2_EDWTE, D2ZL74_9ENTR, D4BK07_9ENTR, B1EFE9_ESCAT, FIS_KLEP3, A0A1Y0LEJ7_TATCI, A0A1Y6GD53_RAOOR, A0A078LLY0_CITKO, Q6Y1R7_CITFR, A0A0D1L4F1_KLEAE, FIS_SALEP, FIS_SALG2, D6IEG9_ECOLX, D6JFR3_ECOLX, A0A1X3JSA2_9ESCH, A0A094XHR4_ENTCL, A0A1I7BM60_9ENTR, FIS_SALPK, F4NHK8_ECOLX, A0A2S7SH82_ESCFE, E0J6U6_ECOLW, S3JL92_9ENTR, A0A0G3PVT1_KLEPN, FIS_ECO27, A0A1R0FS41_CITBR, A0A2T9HT51_SALET, A0A0T9XQU7_SALET, FIS_ECO8A, FIS_ECO45, FIS_ESCF3, FIS_ECO55, FIS_ECOLU, FIS_ECO7I, FIS_ECO81, A0A2T8MFE8_SALAN, A0A0F6B7I1_SALT1, A0A0F5BGD7_SALER, A0A2I5HLZ9_SALDZ, FIS_ECOBW, A0A265B0X1_SALET, FIS_SERMA, Q69I04_SERMA, A0A0F1CZF6_ENTAS, A0A089PFU5_PLUGE, A0A1X0XE98_KLUIN, A0A240CBY3_SERFI, A0A2X5AFI6_SERRU, A0A1Q8MCX4_SHIBO, A0A2S8E3H9_SHIDY, FIS_SHIFL, A0A0I0Y1D4_SHISO, I2B4K6_SHIBC, D8MXQ8_ERWBE, FIS_EDWI9, A0A2A7TZC0_EDWTA, D2TNC0_CITRI, A0A095TNI9_9GAMM, D2TDC5_ERWP6, A0A1X3JD28_ECOLX, A0A1X3KYU1_ECOLX, F4VJQ8_ECOLX, A0A1X3KTG5_ECOLX, A0A1X3I4J2_ECOLX, A0A0K9TH56_ECOLX, F4T400_ECOLX, F4TK28_ECOLX, A0A1X3LEI3_ECOLX, A0A1X3LTB7_ECOLX, A0A1X3IY10_ECOLX, E9XG19_ECOLX, D4HUT9_ERWAC, A0A1X1DWD3_9GAMM, A0A076LM22_9GAMM, D4E129_SEROD, L0MNM9_9GAMM, E6BFW5_ECOLX, E1ILL6_ECOLX, E1J7I0_ECOLX, D8EE67_ECOLX, A0A0H3EM87_ECO8N, A0A1J5WD66_9ENTR, C9XTG0_CROTZ, L0M186_ENTBF, E3GCN0_ENTLS, A0A0H3CSH4_ENTCC, A0A0H3DTV7_EDWTF, A0A2W0VUI8_KLEPN, A0A0E1M236_ECOLX, D8A726_ECOMS, D7XNB8_ECOLX, D7Y126_ECOM1, E9T9W0_ECOLX, I4T2P7_ECOLX, A0A2X5NEL3_9GAMM, A0A2X4V5R4_SERPL, FIS_ECOLI, FIS_ECO57, A0A1V2Z775_9ENTR, R4YI82_KLEPR, I2UH40_ECOLX, I2X0A5_ECOLX, A0A2J7T645_9ENTR, F5S2L5_9ENTR, A0A1S0Z772_SALET, FIS_SALTI, A0A0F7JDQ0_SALTM, E8XD90_SALT4, A0A2T8WWR5_SALET, E9CLN1_9GAMM, A0A250B2G3_9GAMM, J5W609_9ENTR, A0A192CDY0_ECOLX, F0JTD8_ESCFE, FIS_SALTY
KEGG ID ecg:E2348C_3531, sty:STY3565, kpm:KPHS_48020, sfw:WN53_05270, epr:EPYR_00287, cfq:C2U38_23230, eck:EC55989_3675, sply:Q5A_023070, sfo:Z042_09480, ece:Z4621, esc:Entcl_0445, ecp:ECP_3354, ecj:JW3229, sew:SeSA_A3577, stm:STM3385, sdy:SDY_3438, ecm:EcSMS35_3556, pgz:C2E15_02335, ege:EM595_0413, kpv:KPNIH29_23860, etr:ETAE_3152, eci:UTI89_C3703, see:SNSL254_A3649, smaf:D781_4155, kin:AB182_28025, ctu:CTU_03250, elo:EC042_3544, kpw:KPNIH30_24185, seni:CY43_17625, seg:SG3276, sfl:SF3299, ecx:EcHS_A3454, ecz:ECS88_3646, cko:CKO_04673, eco:b3261, stt:t3300, sea:SeAg_B3576, kpy:KPNIH31_23360, kpx:PMK1_01178, sec:SCH_3323, tpty:NCTC11468_00232, cen:LH86_03170, erj:EJP617_14410, tci:A7K98_00845, kps:KPNJ2_00526, cdm:AFK67_01840, eec:EcWSU1_04070, seb:STM474_3547, pge:LG71_01455, ecc:c4027, kpg:KPNIH32_25065, etd:ETAF_2845, kpnu:LI86_02405, esa:ESA_03651, sbg:SBG_3003, ecd:ECDH10B_3436, kpe:KPK_0447, elg:BH714_19465, sey:SL1344_3358, ecq:ECED1_3920, ebi:EbC_40840, kpn:KPN_03670, spe:Spro_4424, elw:ECW_m3527, efe:EFER_3240, eum:ECUMN_3735, cnt:JT31_14480, lni:CWR52_14780, eln:NRG857_16180, kox:KOX_04145, lax:APT61_02340, kqu:AVR78_02190, eclg:EC036_40230, kpk:A593_08545, cama:F384_17920, caf:AL524_00585, kom:HR38_02690, ebd:ECBD_0484, sek:SSPA3038, ecli:ECNIH5_19740, cro:ROD_45531, enc:ECL_04646, ebt:EBL_c03320, ebc:C2U52_10960, eta:ETA_02720, kpr:KPR_4856, spq:SPAB_04219, eae:EAE_04725, ccon:AFK62_01865, kpnk:BN49_0450, ecf:ECH74115_4578, ecs:ECs4133, ebf:D782_0437, enr:H650_12785, ecls:LI67_021810, eno:ECENHK_20045, kvd:KR75_05900, ete:ETEE_1343, ses:SARI_04245, sed:SeD_A3746, seo:STM14_4083, ecl:EcolC_0445, ern:BFV67_19890, ecln:ECNIH4_02565, spt:SPA3252, kpb:FH42_14675, ecv:APECO1_3177, ecw:EcE24377A_3746, sfx:S3516, kpa:KPNJ1_00487, eam:EAMY_0270, ekb:BFV64_21245, sfj:SAMEA4384070_4386, kll:BJF97_26315, kpt:VK055_3805, kmi:VW41_21480, esl:O3K_02660, palh:B1H58_05405, sei:SPC_3456, set:SEN3219, clap:NCTC11466_00281, eic:NT01EI_3506, elh:ETEC_3520, seh:SeHA_C3683, gqu:AWC35_13865, kie:NCTC12125_04368, lei:C2U54_02400, ecy:ECSE_3542, csi:P262_05359, ecr:ECIAI1_3404, ebu:CUC76_17945, sry:M621_23060, cem:LH23_03220, kpne:KU54_002400, kvq:SP68_12510, senl:IY59_17015, ebw:BWG_2960, sbz:A464_3465, ect:ECIAI39_3761
KEGG pathway (determined by BlastKOALA) map05111
PDB ID 1ETK, 1ETO, 3JRD, 1ETW, 5DTD, 6P0T, 3JRE, 1ETV, 3JRB, 5DS9, 3JRH, 6P0U, 1ETQ, 3JRI, 1ETY, 3JRF, 4FIS, 5E3N, 1F36, 1FIP, 3JRC, 5E3O, 1FIA, 1ETX, 3JRG, 3FIS, 6P0S, 3IV5, 3JRA, 5E3M, 3JR9, 5E3L

  Target
Target Regulation (↓/↑) Target sequence
EC042_RS26480 (aggR) promoter ATTAACAAACGTATTTTATATGAGTTAAAAATATATCTTTTTATTGATAAGAGTTAGGTCATTCTAACGCAGATTGCCTGATAAAGACATTTTTTTCATGTGAGAATGATATGAAATTAAAACAAAACATCGAAAAAGA

Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
HTH_8 PF02954.21 2.4e-14 55..95

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-98 MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNGQDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9904 - -



  Protein sequence of regulator: 99 a.a.    .

>REG0249 NC_017626:3772795-3773091 [Escherichia coli 042]
MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNGQDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALM
MGINRGTLRKKLKKYGMN*

  Nucleotide sequence of regulator: 297 bp    .

>REG0249 NC_017626:3772795-3773091 [Escherichia coli 042]
ATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCT
GCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAACGGTCAGGATGTGAATGACCTCTATGAGCTGG
TACTGGCTGAAGTAGAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAACCAGACCCGTGCTGCGCTGATG
ATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAA