Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS01034  |
| T6SS type | Type i1 |
| Strain | Escherichia coli PCN033 |
| Replicon (RefSeq) | chromosome (NZ_CP006632) |
| Location | 246100..267921 |
| Function | anti-bacterial; anti-eukaryotic; anti-adverse environment |
| Comment | T6SS contributes to the pathogenicity and antibacterial activity of porcine extra-intestinal pathogenic Escherichia coli. Hcp family proteins of T6SS in PCN033 contributed to bacterial competition and modulated the bacterial interaction with PK-15 cells during adherence and invasion. |
| References | [1] Peng Y, Wang X, Shou J, Zong B, Zhang Y, Tan J, Chen J, Hu L, Zhu Y, Chen H, Tan C. et al (2016) Roles of Hcp family proteins in the pathogenesis of the porcine extraintestinal pathogenic Escherichia coli type VI secretion system.. Sci Rep. 6:26816. [PudMed:27229766] [2] Zong B, Zhang Y, Wang X, Liu M, Zhang T, Zhu Y, Zheng Y, Hu L, Li P, Chen H, Tan C. et al (2019) Characterization of multiple type-VI secretion system (T6SS) VgrG proteins in the pathogenicity and antibacterial activity of porcine extra-intestinal pathogenic Escherichia coli.. Virulence. 10(1):118-132. [PudMed:30676217] [3] Hu L, Wang C, Lu W, Lu H, Chen H, Tan C. et al (2020) BaeSR activates type VI secretion system expression in porcine extra-intestinal pathogenic Escherichia coli to enhance bacterial resistance to zinc stress.. Microb Pathog. 147:104357. [PudMed:32603765] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssJ | TssK | TssL | TssM |
| Number | 2 | 1 | 1 | 2 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| PPECC33_RS01115 (dnaQ) | 241122..241853 | + | 732 | WP_001366128.1 | DNA polymerase III subunit epsilon | ||
| PPECC33_RS01125 | 242393..243178 | + | 786 | WP_001053625.1 | hypothetical protein | ||
| PPECC33_RS01130 | 243327..243794 | - | 468 | WP_001236629.1 | hypothetical protein | ||
| PPECC33_RS25760 | 243804..244046 | - | 243 | pseudogene | hypothetical protein | ||
| PPECC33_RS01135 | 244060..245268 | - | 1209 | WP_001352368.1 | IS4-like element ISVsa5 family transposase | ||
| PPECC33_RS01140 | 245376..246056 | - | 681 | pseudogene | hypothetical protein | ||
| T6CP013401 | TssD | PPECC33_RS01145 | 246100..246582 | - | 483 | WP_000002621.1 | Hcp family type VI secretion system effector |
| T6CP013402 | TssA | PPECC33_RS01150 | 246606..247958 | - | 1353 | WP_001087587.1 | type VI secretion system ImpA and VasL domain-containing protein |
| T6CP013403 | TssM | PPECC33_RS01155 (tssM) | 247969..251403 | - | 3435 | WP_122986715.1 | type VI secretion system membrane subunit TssM |
| T6CP013404 | TssA | PPECC33_RS01160 (tssA) | 251512..252927 | - | 1416 | WP_001240540.1 | type VI secretion system protein TssA |
| PPECC33_RS01165 (tagO) | 252932..253675 | - | 744 | WP_000088866.1 | type VI secretion system-associated protein TagO | ||
| T6CP013405 | TssH | PPECC33_RS01170 (tssH) | 253672..256455 | - | 2784 | WP_000614409.1 | type VI secretion system ATPase TssH |
| T6CP013406 | TssL | PPECC33_RS01175 (icmH) | 256464..257225 | - | 762 | WP_000343111.1 | type IVB secretion system protein IcmH/DotU |
| T6CP013407 | TssK | PPECC33_RS01180 (tssK) | 257230..258561 | - | 1332 | WP_000246417.1 | type VI secretion system baseplate subunit TssK |
| T6CP013408 | TssJ | PPECC33_RS01185 (tssJ) | 258564..259088 | - | 525 | WP_001080149.1 | type VI secretion system lipoprotein TssJ |
| PPECC33_RS01190 (tagH) | 259085..260365 | - | 1281 | WP_001113718.1 | type VI secretion system-associated FHA domain protein TagH | ||
| T6CP013409 | TssG | PPECC33_RS01195 (tssG) | 260390..261145 | - | 756 | WP_047938329.1 | type VI secretion system baseplate subunit TssG |
| PPECC33_RS01200 (tnpA) | 261236..261694 | - | 459 | WP_000526142.1 | IS200/IS605 family transposase | ||
| T6CP013410 | TssG | PPECC33_RS01205 | 261815..262183 | - | 369 | WP_000348801.1 | type VI secretion system baseplate subunit TssG |
| T6CP013411 | TssF | PPECC33_RS01210 (tssF) | 262147..263997 | - | 1851 | WP_000393830.1 | type VI secretion system baseplate subunit TssF |
| T6CP013412 | TssE | PPECC33_RS01215 (tssE) | 264001..264414 | - | 414 | WP_000611735.1 | type VI secretion system baseplate subunit TssE |
| T6CP013413 | TssC | PPECC33_RS01220 (tssC) | 264421..265896 | - | 1476 | WP_000056997.1 | type VI secretion system contractile sheath large subunit |
| PPECC33_RS01225 | 265965..266171 | - | 207 | WP_000718847.1 | hypothetical protein | ||
| T6CP013414 | TssB | PPECC33_RS01230 (tssB) | 266206..266706 | - | 501 | WP_000037390.1 | type VI secretion system contractile sheath small subunit |
| T6CP013415 | TssD | PPECC33_RS01240 | 267403..267921 | + | 519 | WP_001142958.1 | Hcp family type VI secretion system effector |
| PPECC33_RS01250 | 268131..270238 | + | 2108 | pseudogene | type VI secretion system tip protein VgrG |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0357 |
PPECC33_RS11180 (BaeS) | protein | TCS-HK | Escherichia coli PCN033 | chromosome (NZ_CP006632) | 2271464..2272867 (+) | 446597798 | PPECC33_RS11185 (BaeR) (↓) |
| REG0358 |
PPECC33_RS11185 (BaeR) | protein | TCS-RR | Escherichia coli PCN033 | chromosome (NZ_CP006632) | 2272864..2273586 (+) | 446060022 | T6SS promotor (↓) |
| REG0362 |
PPECC33_RS11945 (rcsB) | protein | TCS-RR | Escherichia coli PCN033 | chromosome (NZ_CP006632) | 2429320..2429970 (+) | 446984661 | T6SS transcription (↓) |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF28262 |
PPECC33_RS01260 (Rhs1) | - | - | Escherichia coli PCN033 | chromosome (NZ_CP006632) | 270305..274567 (+) | WP_047938332.1 |
| EFF28264 |
PPECC33_RS03580 (Rhs3) | - | IMU38222 |
Escherichia coli PCN033 | chromosome (NZ_CP006632) | 774741..778916 (+) | WP_047938335.1 |
| EFF28267 |
PPECC33_RS07930 (Rhs2) | - | IMU38223 |
Escherichia coli PCN033 | chromosome (NZ_CP006632) | 1615760..1619914 (+) | WP_047938343.1 |
| EFF28269 |
PPECC33_RS10745 (Rhs4) | - | - | Escherichia coli PCN033 | chromosome (NZ_CP006632) | 2169467..2173648 (-) | WP_047938391.1 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU38222 |
PPECC33_RS03585 (Rhs3I) | EFF28264 |
Escherichia coli PCN033 | chromosome (NZ_CP006632) | 778901..779275 (+) | WP_000494464.1 |
| IMU38223 |
PPECC33_RS07935 (Rhs2I) | EFF28267 |
Escherichia coli PCN033 | chromosome (NZ_CP006632) | 1619941..1620285 (+) | WP_001279013.1 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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