Detailed information of component protein


Summary

Component ID T6CP013403
Component protein type TssM
T6SS ID (Type) T6SS01034 experimental (Type i1)
Strain Escherichia coli PCN033
Replicon chromosome
Sequence Protein sequence (1145 a.a.); Nucleotide sequence (3435 bp)
Reference
[1] Peng Y, Wang X, Shou J, et al. Roles of Hcp family proteins in the pathogenesis of the porcine extraintestinal pathogenic Escherichia coli type VI secretion system. Sci Rep. 2016 May 27;6:26816. doi: 10.1038/srep26816. PMID: 27229766
[2] Zong B, Zhang Y, Wang X, et al. Characterization of multiple type-VI secretion system (T6SS) VgrG proteins in the pathogenicity and antibacterial activity of porcine extra-intestinal pathogenic Escherichia coli. Virulence. 2019 Dec;10(1):118-132. doi: 10.1080/21505594.2019.1573491. PMID: 30676217
[3] Hu L, Wang C, Lu W, et al. BaeSR activates type VI secretion system expression in porcine extra-intestinal pathogenic Escherichia coli to enhance bacterial resistance to zinc stress. Microb Pathog. 2020 Oct;147:104357. doi: 10.1016/j.micpath.2020.104357. Epub 2020 Jun 27. PMID: 32603765
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PPECC33_RS01155
Coordinate (Strand) 247969..251403 (-)
NCBI ID WP_122986715.1
RefSeq NZ_CP006632
Uniprot ID UPI000F0A482C
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 2.5e-69 476..788
IcmF-related_N PF14331.8 3e-60 175..423
IcmF_C PF06744.14 5.3e-20 1018..1123
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-1 M
Transmembrane helix 2-21 LILAWIFLLVWIWWKGPAWT
Outside 22-30 LHEEQWLKP
Transmembrane helix 31-53 LANRWLATAAWGIVALIWLIVRV
Inside 54-419 MKRLQQLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRHEHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLMVGQSHARASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLMRSRRR
Transmembrane helix 420-442 LTVFSATGGVAALLLIAGWHHYY
Outside 443-1144 NGNYQSGITVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLAEMGLYQGRRIGPYVERTYLQLLEQRYLPSLFNGLVKEMNAAPPESEEKLAVLRVMRMLEDKSGRNNEVVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKPVVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVPQFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDYTATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGTFSEKLSAKERDEALAEPDYQLLTRLGHEFAPENSTLTVQKDKESTMQAVYLQLTELHRYLLAIQNAPVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWAGRLADQAWHVVMVEAVHYMEVDWRDSVVKPFNEQLANNYPFNPHSAQDASLDAFERFFKPDGILDTFYQQNLKLFIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKRRSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLVGARGGAPRSISFSGPWAQFRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9969 - -


  Protein sequence: 1145 a.a.    

>T6CP013403 NZ_CP006632:c251403-247969 [Escherichia coli PCN033] [TssM]
MLILAWIFLLVWIWWKGPAWTLHEEQWLKPLANRWLATAAWGIVALIWLIVRVMKRLQQLEKQQKQQREEAIDPLSVELN
AQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRHEHLLQTLRSRLQDIRQHLHCQLPVYV
VLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLMVGQSHARASLFSFSRQ
MQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALL
AEPNLATESRAWLMRSRRRLTVFSATGGVAALLLIAGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDYGNLQLPLLN
PVRDATLAYGDWGDRSRLAEMGLYQGRRIGPYVERTYLQLLEQRYLPSLFNGLVKEMNAAPPESEEKLAVLRVMRMLEDK
SGRNNEVVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKPVVSAQKELSKLPVYQR
VYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVPQFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKY
SDADRAEIQRQLTEQYISDYTATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGTFSEKLSAKE
RDEALAEPDYQLLTRLGHEFAPENSTLTVQKDKESTMQAVYLQLTELHRYLLAIQNAPVPGKSALKAVQLRLDQNSSDPI
FATRQMAKTLPAPLNRWAGRLADQAWHVVMVEAVHYMEVDWRDSVVKPFNEQLANNYPFNPHSAQDASLDAFERFFKPDG
ILDTFYQQNLKLFIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKRRSVLNLDGQL
VDYSQGRNYTAHLVWPNNMREGNESKLTLVGARGGAPRSISFSGPWAQFRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRV
HTDTEDNPFSGGLFSQFGLSDTLY*

  Nucleotide sequence: 3435 bp    

>T6CP013403 NZ_CP006632:c251403-247969 [Escherichia coli PCN033] [TssM]
CTGCTGATTCTGGCGTGGATTTTTCTGCTGGTATGGATCTGGTGGAAAGGCCCGGCCTGGACACTGCATGAAGAGCAGTG
GCTCAAGCCACTGGCGAACCGCTGGCTGGCCACGGCAGCGTGGGGTATTGTTGCCCTGATATGGCTTATTGTCAGGGTGA
TGAAGCGCCTGCAACAGCTTGAAAAACAGCAAAAACAACAGCGCGAGGAAGCCATTGATCCGCTCAGTGTGGAACTGAAC
GCCCAGCAGCGTTACCTCGACCGCTGGCTGCTGCGCCTGCAACGCCATCTCGACAACCGCCGTTTCCTGTGGCAATTGCC
GTGGTATATGGTCATCGGTCCGGCGGGCAGTGGCAAAACGACACTGCTGCGTGAGGGTTTTCCGTCAGACATTATTTATG
CCCCGGAGGGCGCGCGCGGCGCAGAACAACGCCTGTACCTCACGCCCCATGTCGGTAAACAGGCTGTGATCTTTGATATC
GATGGCACACTCTGTGCCCCCGCTGATGCGGATATCCTGCATCGCCGTCTGTGGGAACATGCCCTCGGCTGGCTGAAGGA
AAAGCGCGCGCGCCAGCCGCTGAATGGGATTATTCTGACACTCGATTTACCCGATCTGCTGACCGCAGACAAACGCCGCC
ACGAGCATCTGTTACAGACGCTACGCAGCCGCTTGCAGGATATACGCCAGCATCTTCACTGCCAGTTACCGGTTTACGTG
GTACTGACCCGGCTTGATTTATTGCAGGGTTTTGCCGCCCTGTTTCAGTCACTGAACAGACAGGATCGCGATGCGATTCT
GGGCGTCACGTTCACCCGCCGTGCCCATGAAAATGATGACTGGCGAACAGAGTTGAATGCTTTCTGGCAGACATGGGTGG
ATCGAATGAATCTGGCGTTGCCGGATCTGATGGTCGGACAGAGCCACGCCCGTGCGTCTTTATTTAGTTTTTCCCGCCAG
ATGCAGGGAAGCCGTGAACCGCTGGTATCACTGCTTGAGGGTCTGCTTGACGGCGAAAATATGAACGTGATGCTGCGTGG
CGTCTATCTCACCTCTTCGCTTCAGCGCGGACAGATGGATGATATTTTCACCCAGTCTGCCGCCCGCCAGTACCGGCTGG
GCAATAACCCACTGGCGTCCTGGCCCCTGGTGGACACCGCGCCCTATTTCACCCGCAGCCTGTTCCCGCAGGCATTACTC
GCAGAGCCTAATCTGGCAACAGAGAGCCGCGCCTGGCTGATGCGTTCCCGTCGCCGCCTGACGGTTTTCTCTGCCACAGG
CGGCGTGGCGGCACTGCTGCTCATTGCCGGCTGGCATCACTATTACAACGGTAACTATCAGTCCGGCATCACCGTGCTTA
AGCAGGCAAAAGCCTTTATGGATGTACCGCCTCCGCAGGGGGAAGATGACTATGGCAACCTGCAACTGCCGCTGCTGAAC
CCGGTACGCGATGCCACTCTGGCCTACGGTGACTGGGGCGACCGCAGCCGCCTTGCGGAGATGGGATTATATCAGGGGCG
ACGCATCGGGCCTTATGTGGAACGGACCTACCTGCAACTGCTGGAGCAACGCTATCTTCCGTCGCTGTTTAACGGGCTGG
TCAAAGAGATGAACGCCGCGCCACCGGAGAGTGAGGAAAAACTCGCCGTGCTGCGCGTGATGCGAATGCTGGAGGATAAG
AGCGGACGTAACAATGAGGTGGTGAAGCAGTATATGGCAAAACGCTGGAGCGAAAAGTTCCACGGCCAGCGCGATATCCA
GGCGCAACTGATGTCCCATCTTGACTACGCGCTGGCTCACACAGACTGGCATGCAGAGCGTCAGGCGGGCGACGGTGACG
CTATCAGCCGCTGGACGCCGTATGACAAGCCCGTTGTGTCAGCACAGAAAGAACTAAGCAAACTACCTGTCTACCAGCGG
GTTTACCAGAGCCTGAAAACGCGGGCGCTGGGCGTTCTTCCTGCCGACCTCAATCTGCGTGACCAGGTAGGGCCAACCTT
TGACCAGGTGTTTACATCTGCCGATGACAATAAACTGGTTGTTCCACAGTTTCTTACCCGTTACGGCCTGCAAAGCTATT
TTGTAAAACAGCGCGATGAACTGGTTGAACTGACGGCGATGGATTCCTGGGTACTCAACCTCACCCGCAGCGTGAAATAC
AGTGACGCCGACCGGGCGGAAATCCAGCGCCAGTTGACCGAGCAGTATATCAGCGACTACACCGCCACCTGGCGGGCCGG
GATGGACAATCTGAATATCCGCAATTTTGAGTCCATCGGACAACTGACCGGGGCGCTGGAGCAGGTTATCAGCGGCGACC
AGCCTTTGCAGCGGGCGCTGACCGTGCTGCGTGACAACACACAACCAGGCACCTTTTCTGAAAAGCTATCTGCTAAAGAA
CGGGATGAGGCCCTGGCTGAACCAGACTACCAGTTACTCACCCGCCTTGGGCATGAGTTCGCCCCGGAAAACAGTACCCT
GACAGTCCAGAAAGATAAAGAAAGCACGATGCAGGCCGTGTACCTGCAGCTCACCGAGTTACACCGCTATCTGCTGGCGA
TCCAGAACGCGCCTGTACCTGGGAAATCGGCACTGAAAGCCGTGCAGTTACGGCTTGATCAGAACAGCAGCGATCCCATA
TTCGCCACCCGCCAGATGGCAAAAACACTGCCTGCTCCGCTCAACCGCTGGGCAGGCAGGCTGGCTGACCAGGCCTGGCA
TGTGGTGATGGTGGAAGCCGTTCACTATATGGAAGTGGACTGGCGCGACAGCGTGGTGAAACCGTTTAACGAGCAACTGG
CAAATAACTATCCGTTTAATCCACATTCTGCACAGGATGCCTCACTGGATGCCTTCGAACGATTCTTTAAACCAGATGGC
ATACTGGATACCTTCTACCAGCAGAACCTAAAGCTGTTTATCGACAATGACCTGAGTCTGGAGGATGGCGATAACAACGT
CATTATTCGCGAAGATATTATTGCGCAACTGGAAACCGCGCAGAAAATCCGTGACATATTCTTCAGCAAACAGAACGGTC
TGGGAACATCCTTTGCCGTGGAAACGGTATCGCTTTCCGGCAACAAACGCCGCAGCGTACTGAACCTTGACGGTCAGTTA
GTCGATTACAGCCAGGGCCGTAACTATACCGCCCATCTGGTCTGGCCTAACAATATGCGCGAAGGCAACGAAAGCAAACT
GACGCTGGTTGGTGCCCGCGGCGGCGCACCGCGCAGTATCAGCTTCAGCGGGCCGTGGGCTCAGTTCCGCCTGTTCGGGG
CCGGACAGCTGACCGGAGTACAGGATGGCAACTTTACCGTGCGCTTTAGTGTGGACGGTGGCGCAATGACCTACCGCGTG
CATACCGACACGGAAGATAACCCGTTCAGCGGTGGGTTGTTCAGCCAGTTTGGCCTGTCAGACACACTGTACTGA