Detailed information of component protein


Summary

Component ID T6CP013408
Component protein type TssJ
T6SS ID (Type) T6SS01034 experimental (Type i1)
Strain Escherichia coli PCN033
Replicon chromosome
Sequence Protein sequence (175 a.a.); Nucleotide sequence (525 bp)
Reference
[1] Peng Y, Wang X, Shou J, et al. Roles of Hcp family proteins in the pathogenesis of the porcine extraintestinal pathogenic Escherichia coli type VI secretion system. Sci Rep. 2016 May 27;6:26816. doi: 10.1038/srep26816. PMID: 27229766
[2] Zong B, Zhang Y, Wang X, et al. Characterization of multiple type-VI secretion system (T6SS) VgrG proteins in the pathogenicity and antibacterial activity of porcine extra-intestinal pathogenic Escherichia coli. Virulence. 2019 Dec;10(1):118-132. doi: 10.1080/21505594.2019.1573491. PMID: 30676217
[3] Hu L, Wang C, Lu W, et al. BaeSR activates type VI secretion system expression in porcine extra-intestinal pathogenic Escherichia coli to enhance bacterial resistance to zinc stress. Microb Pathog. 2020 Oct;147:104357. doi: 10.1016/j.micpath.2020.104357. Epub 2020 Jun 27. PMID: 32603765
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PPECC33_RS01185
Coordinate (Strand) 258564..259088 (-)
NCBI ID WP_001080149.1
RefSeq NZ_CP006632
Uniprot ID A0A0E0Y3I7_ECO1C, L3QIQ2_ECOLX, A0A222QGU2_ECOLX, V0YIZ7_ECOLX, A0A7U9P168_ECOLX, W1ES33_ECOLX, A0A7U6JSK9_9ESCH, A0A3D8XN76_9ENTR, A0A6I4KN30_9ENTR, A0A6H2GI60_9ESCH, A0A7M3S2E7_ECOHS, B8ZYU5_ECOLX, E0J661_ECOLW, A0A170V2Y9_KLEOX, B7LHD2_ECO55, A0A236UEC4_SHIBO, A0A7Z1EPD9_SHIFL, A0A7Z8FFF8_SHISO, A0A1X3JMG5_ECOLX, F4V9Z9_ECOLX, A0A1X3IBC4_ECOLX, E9XQ99_ECOLX, E6BQF9_ECOLX, E1IY72_ECOLX, E1JBR7_ECOLX, D8E9T8_ECOLX, D7XRE2_ECOLX, I2UQI0_ECOLX, I2WT34_ECOLX
KEGG ID elw:ECW_m0225, eck:EC55989_0226, ecx:EcHS_A0232, esl:O3K_20450
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 8.9e-23 45..164
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-174 MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPSEITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYIDHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLVHVRQSSIEMKKEDE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9992 pos: 18-19. LTG-CE. MNRKAFLACVLMCVILTG


  Protein sequence: 175 a.a.    

>T6CP013408 NZ_CP006632:c259088-258564 [Escherichia coli PCN033] [TssJ]
MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPSEITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKL
LAADYDQIASTPLPDVLGKNYIDHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
HVRQSSIEMKKEDE*

  Nucleotide sequence: 525 bp    

>T6CP013408 NZ_CP006632:c259088-258564 [Escherichia coli PCN033] [TssJ]
ATGAACAGGAAGGCGTTTCTGGCCTGCGTGCTGATGTGCGTCATATTAACCGGCTGCGAAACGGCGAAAAAAATCAGCGA
GGTGATCAAAAACCCGGATATCCAGGTCGGGAGCCTGAAGGAGCAACCTTCGGAAATAACCGTCACCCTGCTGACCGAAC
CGGACACCAATACCAATGCGGAGGGTGAATCAGCCGCCGTGGATGTGCAACTGGTGTACCTGACTGACGACTCAAAATTG
CTGGCAGCCGACTATGACCAGATAGCCAGTACGCCGCTACCGGATGTGCTGGGTAAAAACTATATCGACCACCAGGATTT
CAACCTGTTGCCGGACACCATCAAAACCCTGCCGCCGGTAAAGCTGGATGAGAAAACGCAGTTTATTGGTGTCGTGGCCT
ATTTCTCCGATGACCAGGCCACTGAATGGAAACAAATCGAGACGGTGGAAGGAACCGGCCACCATTACCGCCTGCTGGTG
CATGTACGTCAAAGCAGCATCGAAATGAAAAAAGAGGATGAATAA