Detailed information of immunity protein
Summary
| Immunity protein ID | IMU38222  |
| Cognate effector | EFF28264 |
| Related T6SS (Type) | T6SS01034 (Type i1) |
| Strain | Escherichia coli PCN033 |
| Replicon | chromosome |
| Sequence | Protein sequence (125 a.a.); Nucleotide sequence (375 bp) |
| Description | - |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PPECC33_RS03585 (Rhs3I) |
| Coordinates (Strand) | 778901..779275 (+) |
| NCBI ID | WP_000494464.1 |
| RefSeq | NZ_CP006632 |
| Uniprot ID | A0A792T5F1_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-124 | MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKYENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLIFMANFGESRYFKPAKIAMNKNNLIIRTSDIEIAFKITK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9983 | pos: 19-20. VHA-ET. | MFTKLSIAILLAFSAYVHA |
Protein sequence: 125 a.a. .
>IMU38222 NZ_CP006632:778901-779275 [Escherichia coli PCN033]
MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKYENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAG
RTCSLIFMANFGESRYFKPAKIAMNKNNLIIRTSDIEIAFKITK*
MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKYENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAG
RTCSLIFMANFGESRYFKPAKIAMNKNNLIIRTSDIEIAFKITK*
Nucleotide sequence: 375 bp .
>IMU38222 NZ_CP006632:778901-779275 [Escherichia coli PCN033]
ATGTTTACCAAACTAAGTATTGCCATTTTGTTAGCATTTTCTGCATATGTTCATGCGGAAACATCAGCTCCAATATTTTT
TGAAGATGTTGGCAGTATAGACTCTTGGTTTTCAATTAAATACCAAAAATATGAAAACGTGAAGGATGAACAATACCCAG
AAATTCTGATTTATTATGGTGTTAAAGGTTCAGGAATAAATAGAGTTGATGGGTGGTTTTTCTCATGTGCAAAGGCTGGG
AGAACTTGTAGTCTAATTTTTATGGCTAACTTTGGTGAAAGCCGCTATTTTAAACCTGCAAAAATAGCTATGAATAAGAA
TAATTTGATTATAAGGACGAGTGATATAGAAATAGCATTTAAGATAACGAAATAA
ATGTTTACCAAACTAAGTATTGCCATTTTGTTAGCATTTTCTGCATATGTTCATGCGGAAACATCAGCTCCAATATTTTT
TGAAGATGTTGGCAGTATAGACTCTTGGTTTTCAATTAAATACCAAAAATATGAAAACGTGAAGGATGAACAATACCCAG
AAATTCTGATTTATTATGGTGTTAAAGGTTCAGGAATAAATAGAGTTGATGGGTGGTTTTTCTCATGTGCAAAGGCTGGG
AGAACTTGTAGTCTAATTTTTATGGCTAACTTTGGTGAAAGCCGCTATTTTAAACCTGCAAAAATAGCTATGAATAAGAA
TAATTTGATTATAAGGACGAGTGATATAGAAATAGCATTTAAGATAACGAAATAA