Detailed information of component protein


Summary

Component ID T6CP013415
Component protein type TssD
T6SS ID (Type) T6SS01034 experimental (Type i1)
Strain Escherichia coli PCN033
Replicon chromosome
Sequence Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Reference
[1] Peng Y, Wang X, Shou J, et al. Roles of Hcp family proteins in the pathogenesis of the porcine extraintestinal pathogenic Escherichia coli type VI secretion system. Sci Rep. 2016 May 27;6:26816. doi: 10.1038/srep26816. PMID: 27229766
[2] Zong B, Zhang Y, Wang X, et al. Characterization of multiple type-VI secretion system (T6SS) VgrG proteins in the pathogenicity and antibacterial activity of porcine extra-intestinal pathogenic Escherichia coli. Virulence. 2019 Dec;10(1):118-132. doi: 10.1080/21505594.2019.1573491. PMID: 30676217
[3] Hu L, Wang C, Lu W, et al. BaeSR activates type VI secretion system expression in porcine extra-intestinal pathogenic Escherichia coli to enhance bacterial resistance to zinc stress. Microb Pathog. 2020 Oct;147:104357. doi: 10.1016/j.micpath.2020.104357. Epub 2020 Jun 27. PMID: 32603765
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) PPECC33_RS01240
Coordinate (Strand) 267403..267921 (+)
NCBI ID WP_001142958.1
RefSeq NZ_CP006632
Uniprot ID A0A1Z3UUT6_ECOLX, A0A0E0Y4V8_ECO1C, L3QI00_ECOLX, A0A222QFD4_ECOLX, U9YRG6_ECOLX, V0YIY8_ECOLX, A0A7U9J1J4_ECOLX, W1EQY6_ECOLX, A0A3D8XNL5_9ENTR, A0A6I4KHM9_9ENTR, A0A6H2GIT7_9ESCH, A0A7W4PWZ7_9ESCH, A7ZHX3_ECO24, A0A7M3S2F6_ECOHS, A0A0E1SSY5_ECOLX, B6HZU9_ECOSE, A0A0H3PHB8_ECO5C, D6I5D3_ECOLX, O52671_ECOLX, A0A7L6ZRT9_ESCFE, E0J653_ECOLW, A0A170UYG5_KLEOX, B7LHE1_ECO55, A0A7Z1J2J2_SHIFL, A0A7Z1D0C6_SHISO, A0A1X3JLW6_ECOLX, A0A1X3L786_ECOLX, F4VA08_ECOLX, A0A1X3IBM1_ECOLX, F4TA69_ECOLX, A0A1X3LM59_ECOLX, A0A1X3J5C3_ECOLX, E9XQ91_ECOLX, E6BQG9_ECOLX, E1IYR3_ECOLX, E1JBS7_ECOLX, D8E9U8_ECOLX, D3QWN1_ECOCB, D7XRD3_ECOLX, E9TA15_ECOLX, Q7DC35_ECO57, I2UQJ1_ECOLX, I2WRX5_ECOLX, F0JUI5_ESCFE
KEGG ID ecw:EcE24377A_0243, elw:ECW_m0233, eck:EC55989_0235, ecs:ECs0234, ecx:EcHS_A0241, esl:O3K_20405, ecy:ECSE_0232, eok:G2583_0242
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_HCP PF05638.14 5e-32 8..155
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-172 MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEFLHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTSVEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGTSGADDWRAPLEA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9242 - -


  Protein sequence: 173 a.a.    

>T6CP013415 NZ_CP006632:267403-267921 [Escherichia coli PCN033] [TssD]
MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEFLHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPL
MYNALASGEMLPTTELHWWRTSVEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
SGADDWRAPLEA*

  Nucleotide sequence: 519 bp    

>T6CP013415 NZ_CP006632:267403-267921 [Escherichia coli PCN033] [TssD]
ATGCCAACACCGTGTTATATCTCCATCACAGGTCAGACCCAGGGAAATATTACTGCAGGTGCATTTACCGCTGATTCTGT
CGGCAACATCTATGTGCAGGGGCACGAAGACGAGATGCTGGTGCAGGAATTCCTGCATAACGTTACTGTCCCGACCGATC
CTCAGTCCGGCCAGCCTGCGGGTCAGCGTGCTCACAAGCCGTTCATTTTTACCGTGGCGCTGAACAAAGCTGTTCCGCTG
ATGTACAACGCACTGGCCTCCGGCGAAATGCTGCCAACCACTGAACTTCACTGGTGGCGTACCTCTGTGGAAGGCAAACA
GGAGCATTATTTCACCACCCGCCTGACCGATTCGACGATTGTCGATATGAAACTGCATATGCCGCATTGCCAGGATCCGG
CGAAACGCGAGTTCACCCAGTTGCTCGAAGTTTCACTGGCCTACCGCAAAATAGAGTGGGAACACGTCAAGTCTGGCACC
TCCGGTGCCGACGACTGGCGCGCACCGCTGGAAGCATAA