Detailed information of regulator
Summary
| Regulator ID | REG0344  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | QS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | RNA degradation (map03018); Quorum sensing (map02024); Biofilm formation - Vibrio cholerae (map05111) |
| Related T6SS | T6SS01214 (Type Unknown) |
| Strain | Klebsiella pneumoniae strain Kp52.145 |
| Replicon | chromosome |
| Sequence | Protein sequence (103 a.a.); Nucleotide sequence (309 bp) |
| Description | The expression of tssB::lucFF was significantly higher in the h-ns, mgrB and rcsB mutant backgrounds than in the wild-type one and E. coli was not killed when co-incubated with rpoN, rpoS, hfq, fur and pmrAB mutants. |
| Reference |
External database links
| Locus tag (Gene) | BN49_RS26620 (hfq) |
| Coordinates (Strand) | 5173711..5174019 (-) |
| NCBI ID | 488974767 |
| RefSeq | NZ_FO834906 |
| Uniprot ID | A0A0H3GQJ6_KLEPH, V0AUS1_KLEPN, A0A0E1CL72_KLEPN, W8UPJ3_KLEPN, A0A1C1EWG5_9ENTR, A0A1F2M460_9ENTR, A0A223UI03_9ENTR, A0A087FTJ6_KLEVA, HFQ_KLEP7, HFQ_KLEP3, A0A2W0X2F6_KLEPN, R4Y4W4_KLEPR |
| KEGG ID | kpn:KPN_04570, kqu:AVR78_06760, kpe:KPK_5098, kpw:KPNIH30_02275, kps:KPNJ2_05007, kpm:KPHS_04160, kpv:KPNIH29_02240, kpa:KPNJ1_05061, kpt:VK055_2894, kpk:A593_12920, kvd:KR75_10365, kvq:SP68_16955, kpg:KPNIH32_02285, kpr:KPR_0546, kpy:KPNIH31_02300 |
| KEGG pathway (determined by BlastKOALA) | map03018; map02024; map05111 |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| TssB transcription | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Hfq | PF17209.5 | 6.3e-31 | 7..69 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-102 | MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVILLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGSSNYHHGGSAQGSSAPQQDSDDAE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9910 | - | - |
Protein sequence of regulator: 103 a.a. .
>REG0344 NZ_FO834906:c5174019-5173711 [Klebsiella pneumoniae]
MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVILLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGSS
NYHHGGSAQGSSAPQQDSDDAE*
MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVILLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGSS
NYHHGGSAQGSSAPQQDSDDAE*
Nucleotide sequence of regulator: 309 bp .
>REG0344 NZ_FO834906:c5174019-5173711 [Klebsiella pneumoniae]
ATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCTTTGCGTCGGGAACGCGTTCCGGTTTCTATTTATTTGGT
GAATGGTATCAAGCTGCAAGGGCAAATTGAGTCTTTCGATCAGTTCGTGATCCTGTTGAAGAACACGGTCAGCCAGATGG
TCTATAAGCACGCAATTTCTACTGTTGTCCCGTCTCGCCCGGTGTCTCACCACAGCAACAATGCTGGCGGCGGTTCCAGT
AACTATCACCACGGTGGTAGCGCGCAGGGTTCTTCTGCGCCGCAACAAGACAGCGACGACGCCGAATAA
ATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCTTTGCGTCGGGAACGCGTTCCGGTTTCTATTTATTTGGT
GAATGGTATCAAGCTGCAAGGGCAAATTGAGTCTTTCGATCAGTTCGTGATCCTGTTGAAGAACACGGTCAGCCAGATGG
TCTATAAGCACGCAATTTCTACTGTTGTCCCGTCTCGCCCGGTGTCTCACCACAGCAACAATGCTGGCGGCGGTTCCAGT
AACTATCACCACGGTGGTAGCGCGCAGGGTTCTTCTGCGCCGCAACAAGACAGCGACGACGCCGAATAA