Detailed information of Recipient susceptibility factor

Summary

ID	RSF000012
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (176 a.a.); Nucleotide sequence (528 bp)
Response to T6SS attack	protective
Known or putative function	Acid stress response
Description	The mutants with more-pronounced lysis, and then, competition assays with serial plating confirmed their survival was significantly reduced by the TseL-active T6SS-dependent killer
Reference	[1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [3] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

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External database links

Locus tag (Gene)	BW25113_RS18200 (gadE)
Coordinates (Strand)	3651726..3652253 (+)
NCBI ID	446498836
RefSeq	NZ_CP009273
Uniprot ID	A0A1Z3UY72_ECOLX, M9FMU1_ECOLX, A0A0E0XW79_ECO1C, K4X3S6_ECOLX, S1NWV0_ECOLX, L3C485_ECOLX, S1E956_ECOLX, L4IY49_ECOLX, A0A0E2L0D7_ECOU3, A0A029HHU2_ECOLX, GADE_ECOL6, D3H0W0_ECO44, E3PMA2_ECOH1, A7ZT52_ECO24, A0A0E1SRG5_ECOLX, B6I377_ECOSE, B1LJ54_ECOSM, A0A140N6Z5_ECOBD, J7RM85_ECOLX, A0A0H3PX58_ECO5C, D6IF87_ECOLX, D6JGH1_ECOLX, E0IZK9_ECOLW, B7UL65_ECO27, B7MEL4_ECO45, B7L5Z6_ECO55, B7NEF5_ECOLU, A0A0H3MLZ5_ECO7I, B7N1V3_ECO81, A0A1S9JH60_SHIBO, A0A1S9K0K0_SHIDY, GADE_SHIFL, A0A1W2N1L1_SHIFL, A0A0H7K8R4_SHISO, A0A0K9TG51_ECOLX, A0A1X3LD29_ECOLX, A0A1X3LQ58_ECOLX, E9XSV0_ECOLX, A0A0H3EQ74_ECO8N, D3QUE9_ECOCB, A0A0E1M4M1_ECOLX, I4SKK2_ECOLX, GADE_ECOLI, GADE_ECO57, I2SUM9_ECOLX, I2UK53_ECOLX, I2X0J6_ECOLX
KEGG ID	ecg:E2348C_3754, eln:NRG857_17445, ecw:EcE24377A_3998, eco:b3512, ecs:ECs4392, ecq:ECED1_4190, sft:NCTC1_03828, eum:ECUMN_4012, esl:O3K_01350, elw:ECW_m3773, ece:Z4925, ebd:ECBD_0229, ecz:ECS88_3924, ecc:c4323, eck:EC55989_3955, elh:ETEC_3758, eok:G2583_4247, ecj:JW3480, ecm:EcSMS35_3814, ecy:ECSE_3780, elo:EC042_3806, sfl:SF3546, ect:ECIAI39_4014, sfx:S4221
PDB ID	-

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Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GerE	PF00196.21	1.4e-05	117..167

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Transmembrane helices

• Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-175	MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF

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Signal peptides

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9947	-	-

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  Protein sequence: 176 a.a.    .Download


  Nucleotide sequence: 528 bp    .Download