Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000006  |
| Strain | Escherichia coli BW25113 strain K-12 |
| Replicon | chromosome |
| Sequence | Protein sequence (187 a.a.); Nucleotide sequence (561 bp) |
| Response to T6SS attack | protective |
| Known or putative function | Menbtane integrity |
| Description | The mutants with more-pronounced lysis, and then, competition assays with serial plating confirmed their survival was significantly reduced by the TseL-active T6SS-dependent killer |
| Reference | [1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336 |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | BW25113_RS03235 (pagP) |
| Coordinates (Strand) | 652013..652573 (+) |
| NCBI ID | 447025838 |
| RefSeq | NZ_CP009273 |
| Uniprot ID | A0A0E0Y404_ECO1C, K4XLI4_ECOLX, S1ISH0_ECOLX, U9XMZ9_ECOLX, A7ZJ10_ECO24, A0A0E1T3R9_ECOLX, A0A140N9Q1_ECOBD, J7QYK3_ECOLX, F4NLL8_ECOLX, B7L9L5_ECO55, A0A1X3JKJ6_ECOLX, A0A1X3L6C4_ECOLX, F4VB45_ECOLX, F4SKA6_ECOLX, A0A1X3LN02_ECOLX, A0A1X3J599_ECOLX, E6BL31_ECOLX, E1IRC7_ECOLX, E1J1C8_ECOLX, D8E8U2_ECOLX, A0A0E1LWC4_ECOLX, D7XX81_ECOLX, PAGP_ECOLI, I2SYI7_ECOLX, I2WRV0_ECOLX |
| KEGG ID | eco:b0622, eck:EC55989_0615, ecw:EcE24377A_0647, ecj:JW0617, esl:O3K_18480, ebd:ECBD_3028 |
| PDB ID | 1MM4, 1MM5, 1THQ, 3GP6 |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| PagP | PF07017.13 | 1.3e-67 | 37..183 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-186 | MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9594 | pos: 25-26. VFA-NA. | MNVSKYVAIFSFVFIQLISVGKVFA |
Protein sequence: 187 a.a. .
>RSF000006 NZ_CP009273:652013-652573 [Escherichia coli BW25113]
MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGF
GLSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
VTFQMTYIPGTYNNGNVYFAWMRFQF*
MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGF
GLSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
VTFQMTYIPGTYNNGNVYFAWMRFQF*
Nucleotide sequence: 561 bp .
>RSF000006 NZ_CP009273:652013-652573 [Escherichia coli BW25113]
ATGAACGTGAGTAAATATGTCGCTATCTTTTCCTTTGTTTTTATTCAGTTAATCAGCGTTGGTAAAGTTTTTGCTAACGC
AGATGAGTGGATGACAACGTTTAGAGAAAATATTGCACAAACCTGGCAACAGCCTGAACATTATGATTTATATATTCCTG
CCATCACCTGGCATGCACGTTTCGCTTACGACAAAGAAAAAACCGATCGCTATAACGAGCGACCGTGGGGTGGCGGTTTT
GGCCTGTCGCGTTGGGATGAAAAAGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGA
ACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCG
CTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCA
GTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTG
A
ATGAACGTGAGTAAATATGTCGCTATCTTTTCCTTTGTTTTTATTCAGTTAATCAGCGTTGGTAAAGTTTTTGCTAACGC
AGATGAGTGGATGACAACGTTTAGAGAAAATATTGCACAAACCTGGCAACAGCCTGAACATTATGATTTATATATTCCTG
CCATCACCTGGCATGCACGTTTCGCTTACGACAAAGAAAAAACCGATCGCTATAACGAGCGACCGTGGGGTGGCGGTTTT
GGCCTGTCGCGTTGGGATGAAAAAGGAAACTGGCATGGCCTGTATGCCATGGCATTTAAGGACTCGTGGAACAAATGGGA
ACCGATTGCCGGATACGGATGGGAAAGTACCTGGCGACCGCTGGCGGATGAAAATTTTCATTTAGGTCTGGGATTCACCG
CTGGCGTAACGGCACGCGATAACTGGAATTACATCCCTCTCCCGGTTCTACTGCCATTGGCCTCCGTGGGTTATGGCCCA
GTGACTTTTCAGATGACCTACATTCCGGGTACCTACAACAATGGCAATGTGTACTTTGCCTGGATGCGCTTTCAGTTTTG
A