Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00056  |
| Cognate effector | EFF01255 |
| Related T6SS (Type) | T6SS00395 (Type i3) |
| Strain | Enterobacter cloacae subsp. cloacae ATCC 13047 |
| Replicon | chromosome |
| Sequence | Protein sequence (188 a.a.); Nucleotide sequence (564 bp) |
| Description | rhsIB |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | ECL_03139 (RhsIB) |
| Coordinates (Strand) | 3199746..3200309 (-) |
| NCBI ID | 296103478 |
| RefSeq | NC_014121 |
| Uniprot ID | A0A0H3CNC7_ENTCC |
| KEGG ID | enc:ECL_03139 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-187 | MSEIEESLKKLVVICKKHSLPGSFNTVNDLDNVFPSNLPRSTDVDYLYKNYNPEQLKIETGFTPIKIHSVSELSKAQNGYEYLPNNYLVIGDDLGGGKPIIAVVDEGNTSIYASYDVIEPFKIAGSLSGFIFSLAELIDLVYGQYDIFDIADDNDEVKDDFIDELRKRIVPLIGNENFNAFYDYFYG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9856 | - | - |
Protein sequence: 188 a.a. .
>IMU00056 NC_014121:c3200309-3199746 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MSEIEESLKKLVVICKKHSLPGSFNTVNDLDNVFPSNLPRSTDVDYLYKNYNPEQLKIETGFTPIKIHSVSELSKAQNGY
EYLPNNYLVIGDDLGGGKPIIAVVDEGNTSIYASYDVIEPFKIAGSLSGFIFSLAELIDLVYGQYDIFDIADDNDEVKDD
FIDELRKRIVPLIGNENFNAFYDYFYG*
MSEIEESLKKLVVICKKHSLPGSFNTVNDLDNVFPSNLPRSTDVDYLYKNYNPEQLKIETGFTPIKIHSVSELSKAQNGY
EYLPNNYLVIGDDLGGGKPIIAVVDEGNTSIYASYDVIEPFKIAGSLSGFIFSLAELIDLVYGQYDIFDIADDNDEVKDD
FIDELRKRIVPLIGNENFNAFYDYFYG*
Nucleotide sequence: 564 bp .
>IMU00056 NC_014121:c3200309-3199746 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGAGTGAAATTGAAGAGTCTTTAAAAAAACTTGTTGTTATTTGCAAAAAACACTCGCTGCCCGGGAGTTTCAATACAGT
CAATGACTTGGATAATGTATTCCCATCCAACTTACCTCGTTCAACAGATGTTGATTACTTATATAAAAATTATAATCCTG
AACAGTTAAAAATTGAAACAGGCTTCACGCCAATTAAAATTCACTCTGTCAGTGAGCTCTCGAAGGCTCAAAATGGTTAT
GAGTACTTACCAAACAATTACCTTGTTATTGGTGATGATTTAGGTGGAGGTAAACCTATAATTGCGGTTGTCGATGAAGG
GAATACCTCTATATATGCAAGTTATGATGTAATTGAACCATTTAAAATAGCTGGCAGTCTCTCTGGATTTATATTTTCAT
TGGCTGAGCTTATTGATTTGGTATATGGCCAATATGATATCTTTGATATAGCAGATGATAATGACGAAGTAAAAGATGAT
TTTATTGATGAGTTGCGGAAAAGAATTGTGCCTCTTATCGGTAATGAAAATTTCAATGCTTTCTATGATTATTTTTATGG
CTAG
ATGAGTGAAATTGAAGAGTCTTTAAAAAAACTTGTTGTTATTTGCAAAAAACACTCGCTGCCCGGGAGTTTCAATACAGT
CAATGACTTGGATAATGTATTCCCATCCAACTTACCTCGTTCAACAGATGTTGATTACTTATATAAAAATTATAATCCTG
AACAGTTAAAAATTGAAACAGGCTTCACGCCAATTAAAATTCACTCTGTCAGTGAGCTCTCGAAGGCTCAAAATGGTTAT
GAGTACTTACCAAACAATTACCTTGTTATTGGTGATGATTTAGGTGGAGGTAAACCTATAATTGCGGTTGTCGATGAAGG
GAATACCTCTATATATGCAAGTTATGATGTAATTGAACCATTTAAAATAGCTGGCAGTCTCTCTGGATTTATATTTTCAT
TGGCTGAGCTTATTGATTTGGTATATGGCCAATATGATATCTTTGATATAGCAGATGATAATGACGAAGTAAAAGATGAT
TTTATTGATGAGTTGCGGAAAAGAATTGTGCCTCTTATCGGTAATGAAAATTTCAATGCTTTCTATGATTATTTTTATGG
CTAG