Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00395  |
| T6SS type | Type i3 |
| Strain | Enterobacter cloacae subsp. cloacae ATCC 13047 |
| Replicon (RefSeq) | chromosome (NC_014121) |
| Location | 1551980..1588894 |
| Function | anti-bacterial; anti-eukaryotic; effector translocation |
| Comment | T6SS-1 is required for bacterial competition. Killing of E. coli by E. cloacae and Hcp1 protein secretion are dependent on a functional T6SS-1. Hcp1 protein secretion, which is T6SS-1 dependent, confers hemolytic activity to E. cloacae. |
| References | [1] Zhang H et al (2013) Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein. J Biol Chem. 288(8):5928-39. [PudMed:23288853] [2] Whitney JC et al (2014) Genetically distinct pathways guide effector export through the type VI secretion system. Mol Microbiol. 92(3):529-42. [PudMed:24589350] [3] Zhang H et al (2013) Insights into the Cross-Immunity Mechanism within Effector Families of Bacteria Type VI Secretion System from the Structure of StTae4-EcTai4 Complex. PLoS One. 8(9):e73782. [PudMed:24023903] [4] Russell AB et al (2012) A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806] [5] Soria-Bustos J, Ares MA, G��mez-Aldapa CA, Gonz��lez-Y-Merchand JA, Gir��n JA, De la Cruz MA. et al (2020) Two Type VI Secretion Systems of Enterobacter cloacae Are Required for Bacterial Competition, Cell Adherence, and Intestinal Colonization.. Front Microbiol. 11:560488. [PudMed:33072020] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | FHA | TagJ | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| ECL_RS07500 | 1547554..1548345 | + | 792 | WP_044158197.1 | PhnD/SsuA/transferrin family substrate-binding protein | ||
| ECL_RS07505 | 1548311..1550599 | - | 2289 | WP_013096172.1 | FdhF/YdeP family oxidoreductase | ||
| ECL_RS07510 | 1551462..1551956 | + | 495 | WP_013096174.1 | hypothetical protein | ||
| T6CP004055 | TssJ | ECL_RS07515 (tssJ) | 1551980..1552483 | + | 504 | WP_013096175.1 | type VI secretion system lipoprotein TssJ |
| T6CP004056 | TssK | ECL_RS07520 (tssK) | 1552509..1553852 | + | 1344 | WP_013096176.1 | type VI secretion system baseplate subunit TssK |
| T6CP004057 | TssL | ECL_RS07525 | 1553870..1555108 | + | 1239 | WP_013096177.1 | DotU family type VI secretion system protein |
| T6CP004058 | TssM | ECL_RS07530 (tssM) | 1555111..1558731 | + | 3621 | WP_013096178.1 | type VI secretion system membrane subunit TssM |
| ECL_RS07535 (tagF) | 1558748..1559464 | + | 717 | WP_013096179.1 | type VI secretion system-associated protein TagF | ||
| T6CP004059 | TssA | ECL_RS07540 (tssA) | 1559476..1560492 | + | 1017 | WP_013096180.1 | type VI secretion system protein TssA |
| T6CP004060 | TssB | ECL_RS07545 (tssB) | 1560568..1561092 | + | 525 | WP_008502644.1 | type VI secretion system contractile sheath small subunit |
| T6CP004061 | TssC | ECL_RS07550 (tssC) | 1561096..1562595 | + | 1500 | WP_013096181.1 | type VI secretion system contractile sheath large subunit |
| T6CP004062 | TssD | ECL_RS07555 | 1562931..1563413 | + | 483 | WP_008502640.1 | type VI secretion system tube protein Hcp |
| ECL_RS07560 | 1563472..1563963 | + | 492 | WP_013096182.1 | type VI secretion system amidase effector protein Tae4 | ||
| ECL_RS07565 | 1563960..1564313 | + | 354 | WP_013096183.1 | hypothetical protein | ||
| T6CP011829 | FHA | ECL_RS07570 (tagH) | 1564401..1566170 | + | 1770 | WP_013096184.1 | type VI secretion system-associated FHA domain protein TagH |
| ECL_RS07575 | 1566167..1566964 | + | 798 | WP_013096185.1 | protein phosphatase 2C domain-containing protein | ||
| ECL_RS07580 | 1566981..1567967 | + | 987 | WP_013096186.1 | hypothetical protein | ||
| T6CP012788 | TagJ | ECL_RS07585 | 1567977..1568789 | + | 813 | WP_013096187.1 | type VI secretion system protein ImpE |
| T6CP004063 | TssE | ECL_RS07590 (tssE) | 1568782..1569345 | + | 564 | WP_013096188.1 | type VI secretion system baseplate subunit TssE |
| T6CP004064 | TssF | ECL_RS07595 (tssF) | 1569348..1571219 | + | 1872 | WP_038420938.1 | type VI secretion system baseplate subunit TssF |
| T6CP004065 | TssG | ECL_RS07600 (tssG) | 1571216..1572259 | + | 1044 | WP_013096190.1 | type VI secretion system baseplate subunit TssG |
| T6CP004066 | TssH | ECL_RS07605 (tssH) | 1572298..1574913 | + | 2616 | WP_013096191.1 | type VI secretion system ATPase TssH |
| ECL_RS07610 | 1574938..1576371 | + | 1434 | WP_013096192.1 | serine/threonine protein kinase | ||
| ECL_RS07615 | 1576408..1577826 | + | 1419 | WP_013096193.1 | hypothetical protein | ||
| ECL_RS07620 | 1577823..1578584 | + | 762 | WP_013096194.1 | ankyrin repeat domain-containing protein | ||
| ECL_RS07625 | 1578901..1579350 | + | 450 | WP_164928058.1 | hypothetical protein | ||
| ECL_RS07630 | 1579446..1580414 | + | 969 | WP_013087110.1 | IS5-like element IS903B family transposase | ||
| ECL_RS07635 | 1580667..1581140 | + | 474 | pseudogene | hypothetical protein | ||
| ECL_RS07640 | 1581563..1581910 | + | 348 | WP_013096196.1 | hypothetical protein | ||
| T6CP004067 | TssI | ECL_RS07645 (tssI) | 1582007..1583935 | + | 1929 | WP_013096197.1 | type VI secretion system tip protein VgrG |
| ECL_RS07650 | 1583985..1584428 | + | 444 | WP_082208141.1 | ADP-ribosylglycohydrolase family protein | ||
| ECL_RS07655 | 1584430..1585398 | + | 969 | WP_013087110.1 | IS5-like element IS903B family transposase | ||
| ECL_RS07660 | 1585456..1586151 | + | 696 | pseudogene | ADP-ribosylglycohydrolase family protein | ||
| ECL_RS07665 | 1586297..1586730 | + | 434 | pseudogene | hypothetical protein | ||
| ECL_RS07670 (tssI) | 1586973..1588894 | + | 1922 | pseudogene | type VI secretion system tip protein VgrG | ||
| ECL_RS07675 | 1588968..1589387 | + | 420 | WP_052429247.1 | hypothetical protein | ||
| ECL_RS07680 | 1589380..1589943 | + | 564 | WP_044158317.1 | hypothetical protein | ||
| ECL_RS07685 | 1589947..1591023 | + | 1077 | WP_013096203.1 | hypothetical protein | ||
| ECL_RS07690 | 1591099..1591539 | + | 441 | WP_044158315.1 | DUF1795 domain-containing protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF01418 |
ECL_01542 (Tae4) | Tae | IMU00269 |
Enterobacter cloacae subsp. cloacae ATCC 13047 | chromosome (NC_014121) | 1563472..1563963 (+) | 502861206 |
| EFF01872 |
ECL_01553 (Tle) | Tle | IMU00375 |
Enterobacter cloacae subsp. cloacae ATCC 13047 | chromosome (NC_014121) | 1576408..1577826 (+) | 502861217 |
| EFF01254 |
ECL_01567 (RhsA) | Tse | IMU00055 |
Enterobacter cloacae subsp. cloacae ATCC 13047 | chromosome (NC_014121) | 1591543..1595931 (+) | 296101929 |
| EFF01255 |
ECL_03140 (RhsB) | Tse | IMU00056 |
Enterobacter cloacae subsp. cloacae ATCC 13047 | chromosome (NC_014121) | 3200343..3204599 (-) | 296103479 |
| EFF00505 |
ECL_01541 (hcp) | - | - | Enterobacter cloacae subsp. cloacae ATCC 13047 | chromosome (NC_014121) | 1562931..1563413 (+) | 495778061 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU00055 |
ECL_01568 (RhsIA) | EFF01254 |
Enterobacter cloacae subsp. cloacae ATCC 13047 | chromosome (NC_014121) | 1595931..1596929 (+) | 296101930 |
| IMU00056 |
ECL_03139 (RhsIB) | EFF01255 |
Enterobacter cloacae subsp. cloacae ATCC 13047 | chromosome (NC_014121) | 3199746..3200309 (-) | 296103478 |
| IMU00269 |
ECL_01543 (Tai4) | EFF01418 |
Enterobacter cloacae subsp. cloacae ATCC 13047 | chromosome (NC_014121) | 1563960..1564313 (+) | 296101905 |
| IMU00375 |
ECL_01554 (Tli) | EFF01872 |
Enterobacter cloacae subsp. cloacae ATCC 13047 | chromosome (NC_014121) | 1577823..1578584 (+) | 296101916 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|