Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


T6SS ID T6SS00395 experimental
T6SS type Type i3
Strain Enterobacter cloacae subsp. cloacae ATCC 13047
Replicon (RefSeq) chromosome (NC_014121)
Location 1551980..1588894
Function anti-bacterial; anti-eukaryotic; effector translocation
Comment T6SS-1 is required for bacterial competition. Killing of E. coli by E. cloacae and Hcp1 protein secretion are dependent on a functional T6SS-1. Hcp1 protein secretion, which is T6SS-1 dependent, confers hemolytic activity to E. cloacae.
[1] Zhang H et al (2013) Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein. J Biol Chem. 288(8):5928-39. [PudMed:23288853]
[2] Whitney JC et al (2014) Genetically distinct pathways guide effector export through the type VI secretion system. Mol Microbiol. 92(3):529-42. [PudMed:24589350]
[3] Zhang H et al (2013) Insights into the Cross-Immunity Mechanism within Effector Families of Bacteria Type VI Secretion System from the Structure of StTae4-EcTai4 Complex. PLoS One. 8(9):e73782. [PudMed:24023903]
[4] Russell AB et al (2012) A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 11(5):538-49. [PudMed:22607806]
[5] Soria-Bustos J, Ares MA, G��mez-Aldapa CA, Gonz��lez-Y-Merchand JA, Gir��n JA, De la Cruz MA. et al (2020) Two Type VI Secretion Systems of Enterobacter cloacae Are Required for Bacterial Competition, Cell Adherence, and Intestinal Colonization.. Front Microbiol. 11:560488. [PudMed:33072020]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
ECL_RS07500 1547554..1548345 + 792 WP_044158197.1 PhnD/SsuA/transferrin family substrate-binding protein
ECL_RS07505 1548311..1550599 - 2289 WP_013096172.1 FdhF/YdeP family oxidoreductase
ECL_RS07510 1551462..1551956 + 495 WP_013096174.1 hypothetical protein
T6CP004055 TssJ ECL_RS07515 (tssJ) 1551980..1552483 + 504 WP_013096175.1 type VI secretion system lipoprotein TssJ
T6CP004056 TssK ECL_RS07520 (tssK) 1552509..1553852 + 1344 WP_013096176.1 type VI secretion system baseplate subunit TssK
T6CP004057 TssL ECL_RS07525 1553870..1555108 + 1239 WP_013096177.1 DotU family type VI secretion system protein
T6CP004058 TssM ECL_RS07530 (tssM) 1555111..1558731 + 3621 WP_013096178.1 type VI secretion system membrane subunit TssM
ECL_RS07535 (tagF) 1558748..1559464 + 717 WP_013096179.1 type VI secretion system-associated protein TagF
T6CP004059 TssA ECL_RS07540 (tssA) 1559476..1560492 + 1017 WP_013096180.1 type VI secretion system protein TssA
T6CP004060 TssB ECL_RS07545 (tssB) 1560568..1561092 + 525 WP_008502644.1 type VI secretion system contractile sheath small subunit
T6CP004061 TssC ECL_RS07550 (tssC) 1561096..1562595 + 1500 WP_013096181.1 type VI secretion system contractile sheath large subunit
T6CP004062 TssD ECL_RS07555 1562931..1563413 + 483 WP_008502640.1 type VI secretion system tube protein Hcp
ECL_RS07560 1563472..1563963 + 492 WP_013096182.1 type VI secretion system amidase effector protein Tae4
ECL_RS07565 1563960..1564313 + 354 WP_013096183.1 hypothetical protein
T6CP011829 FHA ECL_RS07570 (tagH) 1564401..1566170 + 1770 WP_013096184.1 type VI secretion system-associated FHA domain protein TagH
ECL_RS07575 1566167..1566964 + 798 WP_013096185.1 protein phosphatase 2C domain-containing protein
ECL_RS07580 1566981..1567967 + 987 WP_013096186.1 hypothetical protein
T6CP012788 TagJ ECL_RS07585 1567977..1568789 + 813 WP_013096187.1 type VI secretion system protein ImpE
T6CP004063 TssE ECL_RS07590 (tssE) 1568782..1569345 + 564 WP_013096188.1 type VI secretion system baseplate subunit TssE
T6CP004064 TssF ECL_RS07595 (tssF) 1569348..1571219 + 1872 WP_038420938.1 type VI secretion system baseplate subunit TssF
T6CP004065 TssG ECL_RS07600 (tssG) 1571216..1572259 + 1044 WP_013096190.1 type VI secretion system baseplate subunit TssG
T6CP004066 TssH ECL_RS07605 (tssH) 1572298..1574913 + 2616 WP_013096191.1 type VI secretion system ATPase TssH
ECL_RS07610 1574938..1576371 + 1434 WP_013096192.1 serine/threonine protein kinase
ECL_RS07615 1576408..1577826 + 1419 WP_013096193.1 hypothetical protein
ECL_RS07620 1577823..1578584 + 762 WP_013096194.1 ankyrin repeat domain-containing protein
ECL_RS07625 1578901..1579350 + 450 WP_164928058.1 hypothetical protein
ECL_RS07630 1579446..1580414 + 969 WP_013087110.1 IS5-like element IS903B family transposase
ECL_RS07635 1580667..1581140 + 474 pseudogene hypothetical protein
ECL_RS07640 1581563..1581910 + 348 WP_013096196.1 hypothetical protein
T6CP004067 TssI ECL_RS07645 (tssI) 1582007..1583935 + 1929 WP_013096197.1 type VI secretion system tip protein VgrG
ECL_RS07650 1583985..1584428 + 444 WP_082208141.1 ADP-ribosylglycohydrolase family protein
ECL_RS07655 1584430..1585398 + 969 WP_013087110.1 IS5-like element IS903B family transposase
ECL_RS07660 1585456..1586151 + 696 pseudogene ADP-ribosylglycohydrolase family protein
ECL_RS07665 1586297..1586730 + 434 pseudogene hypothetical protein
ECL_RS07670 (tssI) 1586973..1588894 + 1922 pseudogene type VI secretion system tip protein VgrG
ECL_RS07675 1588968..1589387 + 420 WP_052429247.1 hypothetical protein
ECL_RS07680 1589380..1589943 + 564 WP_044158317.1 hypothetical protein
ECL_RS07685 1589947..1591023 + 1077 WP_013096203.1 hypothetical protein
ECL_RS07690 1591099..1591539 + 441 WP_044158315.1 DUF1795 domain-containing protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files


Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector