Detailed information of immunity protein


Summary

Immunity protein ID IMU00269 experimental
Cognate effector EFF01418 experimental
Related T6SS (Type) T6SS00395 experimental (Type i3)
Strain Enterobacter cloacae subsp. cloacae ATCC 13047
Replicon chromosome
Sequence Protein sequence (118 a.a.); Nucleotide sequence (354 bp)
Description Wild-type ECL has a slight growth advantage over Δtae4 Δtai4 cells, which is abrogated when targets are provided with plasmid- borne tai4
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) ECL_01543 (Tai4)
Coordinates (Strand) 1563960..1564313 (+)
NCBI ID 296101905
RefSeq NC_014121
Uniprot ID A0A0H3CIX8_ENTCC
KEGG ID enc:ECL_01543
PDB ID 4HFK, 4JUR
Pfam domain hit(s)

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Domain
Pfam ID
E-value
Aligned region
Tai4PF16695.74.3e-0931..110
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-117MKKPLLFVLAFVASAANAQTLPDISTFSQQQIFENWVQNRCIGKIADSKSLKEDADASAAAWLEASNLPAENFEKADEVIVSLLKQKVGGTEPGHYQILKCTLIANSDAIRPLKSSK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
SP(Sec/SPI)0.9965pos: 18-19. ANA-QT.MKKPLLFVLAFVASAANA


  Protein sequence: 118 a.a.    .

>IMU00269 NC_014121:1563960-1564313 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MKKPLLFVLAFVASAANAQTLPDISTFSQQQIFENWVQNRCIGKIADSKSLKEDADASAAAWLEASNLPAENFEKADEVI
VSLLKQKVGGTEPGHYQILKCTLIANSDAIRPLKSSK*

  Nucleotide sequence: 354 bp    .

>IMU00269 NC_014121:1563960-1564313 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGAAAAAGCCATTGCTCTTTGTCTTAGCCTTCGTTGCCTCCGCCGCAAATGCCCAGACGTTGCCTGATATCAGTACTTT
TTCTCAACAACAAATCTTCGAAAACTGGGTGCAGAATCGATGCATCGGTAAGATTGCGGACAGTAAAAGCCTGAAAGAAG
ATGCTGACGCCAGCGCCGCGGCATGGCTGGAAGCAAGCAATCTGCCTGCTGAGAATTTTGAAAAAGCTGATGAGGTGATT
GTTTCTTTACTGAAACAGAAGGTAGGTGGTACCGAGCCTGGTCATTATCAGATATTGAAGTGCACCTTGATCGCTAATAG
TGACGCTATTCGACCACTCAAATCCTCAAAGTAG