Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00269  |
| Cognate effector | EFF01418 |
| Related T6SS (Type) | T6SS00395 (Type i3) |
| Strain | Enterobacter cloacae subsp. cloacae ATCC 13047 |
| Replicon | chromosome |
| Sequence | Protein sequence (118 a.a.); Nucleotide sequence (354 bp) |
| Description | Wild-type ECL has a slight growth advantage over Δtae4 Δtai4 cells, which is abrogated when targets are provided with plasmid- borne tai4 |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | ECL_01543 (Tai4) |
| Coordinates (Strand) | 1563960..1564313 (+) |
| NCBI ID | 296101905 |
| RefSeq | NC_014121 |
| Uniprot ID | A0A0H3CIX8_ENTCC |
| KEGG ID | enc:ECL_01543 |
| PDB ID | 4HFK, 4JUR |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Tai4 | PF16695.7 | 4.3e-09 | 31..110 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-117 | MKKPLLFVLAFVASAANAQTLPDISTFSQQQIFENWVQNRCIGKIADSKSLKEDADASAAAWLEASNLPAENFEKADEVIVSLLKQKVGGTEPGHYQILKCTLIANSDAIRPLKSSK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9965 | pos: 18-19. ANA-QT. | MKKPLLFVLAFVASAANA |
Protein sequence: 118 a.a. .
>IMU00269 NC_014121:1563960-1564313 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MKKPLLFVLAFVASAANAQTLPDISTFSQQQIFENWVQNRCIGKIADSKSLKEDADASAAAWLEASNLPAENFEKADEVI
VSLLKQKVGGTEPGHYQILKCTLIANSDAIRPLKSSK*
MKKPLLFVLAFVASAANAQTLPDISTFSQQQIFENWVQNRCIGKIADSKSLKEDADASAAAWLEASNLPAENFEKADEVI
VSLLKQKVGGTEPGHYQILKCTLIANSDAIRPLKSSK*
Nucleotide sequence: 354 bp .
>IMU00269 NC_014121:1563960-1564313 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGAAAAAGCCATTGCTCTTTGTCTTAGCCTTCGTTGCCTCCGCCGCAAATGCCCAGACGTTGCCTGATATCAGTACTTT
TTCTCAACAACAAATCTTCGAAAACTGGGTGCAGAATCGATGCATCGGTAAGATTGCGGACAGTAAAAGCCTGAAAGAAG
ATGCTGACGCCAGCGCCGCGGCATGGCTGGAAGCAAGCAATCTGCCTGCTGAGAATTTTGAAAAAGCTGATGAGGTGATT
GTTTCTTTACTGAAACAGAAGGTAGGTGGTACCGAGCCTGGTCATTATCAGATATTGAAGTGCACCTTGATCGCTAATAG
TGACGCTATTCGACCACTCAAATCCTCAAAGTAG
ATGAAAAAGCCATTGCTCTTTGTCTTAGCCTTCGTTGCCTCCGCCGCAAATGCCCAGACGTTGCCTGATATCAGTACTTT
TTCTCAACAACAAATCTTCGAAAACTGGGTGCAGAATCGATGCATCGGTAAGATTGCGGACAGTAAAAGCCTGAAAGAAG
ATGCTGACGCCAGCGCCGCGGCATGGCTGGAAGCAAGCAATCTGCCTGCTGAGAATTTTGAAAAAGCTGATGAGGTGATT
GTTTCTTTACTGAAACAGAAGGTAGGTGGTACCGAGCCTGGTCATTATCAGATATTGAAGTGCACCTTGATCGCTAATAG
TGACGCTATTCGACCACTCAAATCCTCAAAGTAG