Detailed information of effector
Summary
| Effector ID | EFF01418  |
| Effector type | Tae |
| Cognate immunity protein | IMU00269 |
| Related T6SS (Type) | T6SS00395 (Type i3) |
| Strain | Enterobacter cloacae subsp. cloacae ATCC 13047 |
| Replicon | chromosome |
| Sequence | Protein sequence (164 a.a.); Nucleotide sequence (492 bp) |
| Description | Wild-type ECL has a slight growth advantage over Δtae4 Δtai4 cells, which is abrogated when targets are provided with plasmid- borne tai4 |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | ECL_01542 (Tae4) |
| Coordinates (Strand) | 1563472..1563963 (+) |
| NCBI ID | 502861206 |
| RefSeq | NC_014121 |
| Uniprot ID | A0A0M7EMX8_ENTCL, A0A0H3CIJ2_ENTCC |
| KEGG ID | enc:ECL_01542 |
| PDB ID | 4HFK, 4HFL |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Tae4 | PF14113.8 | 6.2e-22 | 43..139 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-163 | MSHMRPAFGAAWNRFKEVNVNVEQVGKLLGGKVQHNIDAGIFKNACPIRMSYVLNYCGIPVPSNSKYATVTGSDKKRYMFRVKDMIAFLPTVLGKADISVSSPTPAQFAGKQGIIIFTGHGWLDATGHVTLWNGNICSDDCHFLGSPGNGSFIPTNATFWSLK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9741 | - | - |
Protein sequence: 164 a.a. .
>EFF01418 NC_014121:1563472-1563963 Type VI Amidase Effector (Tae) [Enterobacter cloacae subsp. cloacae ATCC 13047]
MSHMRPAFGAAWNRFKEVNVNVEQVGKLLGGKVQHNIDAGIFKNACPIRMSYVLNYCGIPVPSNSKYATVTGSDKKRYMF
RVKDMIAFLPTVLGKADISVSSPTPAQFAGKQGIIIFTGHGWLDATGHVTLWNGNICSDDCHFLGSPGNGSFIPTNATFW
SLK*
MSHMRPAFGAAWNRFKEVNVNVEQVGKLLGGKVQHNIDAGIFKNACPIRMSYVLNYCGIPVPSNSKYATVTGSDKKRYMF
RVKDMIAFLPTVLGKADISVSSPTPAQFAGKQGIIIFTGHGWLDATGHVTLWNGNICSDDCHFLGSPGNGSFIPTNATFW
SLK*
Nucleotide sequence: 492 bp .
>EFF01418 NC_014121:1563472-1563963 Type VI Amidase Effector (Tae) [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGTCTCATATGCGTCCTGCTTTTGGTGCCGCCTGGAACCGATTTAAAGAAGTGAACGTCAACGTAGAACAGGTTGGCAA
GTTATTGGGGGGGAAAGTTCAGCATAATATAGATGCTGGTATTTTTAAAAATGCCTGCCCCATTCGTATGAGCTATGTTT
TAAATTATTGTGGCATTCCAGTTCCTTCTAATAGTAAATATGCAACTGTGACCGGGAGCGACAAGAAGCGATATATGTTC
CGTGTTAAAGATATGATTGCTTTTTTACCCACGGTATTGGGAAAAGCTGACATATCTGTTTCTTCTCCAACCCCAGCACA
ATTTGCGGGCAAGCAAGGTATTATTATTTTTACCGGGCATGGCTGGCTTGATGCAACCGGGCATGTAACACTCTGGAACG
GAAATATTTGTTCGGATGATTGTCATTTCCTTGGCTCACCGGGTAATGGTTCATTTATCCCTACTAACGCAACCTTCTGG
AGCCTGAAATGA
ATGTCTCATATGCGTCCTGCTTTTGGTGCCGCCTGGAACCGATTTAAAGAAGTGAACGTCAACGTAGAACAGGTTGGCAA
GTTATTGGGGGGGAAAGTTCAGCATAATATAGATGCTGGTATTTTTAAAAATGCCTGCCCCATTCGTATGAGCTATGTTT
TAAATTATTGTGGCATTCCAGTTCCTTCTAATAGTAAATATGCAACTGTGACCGGGAGCGACAAGAAGCGATATATGTTC
CGTGTTAAAGATATGATTGCTTTTTTACCCACGGTATTGGGAAAAGCTGACATATCTGTTTCTTCTCCAACCCCAGCACA
ATTTGCGGGCAAGCAAGGTATTATTATTTTTACCGGGCATGGCTGGCTTGATGCAACCGGGCATGTAACACTCTGGAACG
GAAATATTTGTTCGGATGATTGTCATTTCCTTGGCTCACCGGGTAATGGTTCATTTATCCCTACTAACGCAACCTTCTGG
AGCCTGAAATGA