Detailed information of component protein


Summary

Component ID T6CP004058
Component protein type TssM
T6SS ID (Type) T6SS00395 experimental (Type i3)
Strain Enterobacter cloacae subsp. cloacae ATCC 13047
Replicon chromosome
Sequence Protein sequence (1207 a.a.); Nucleotide sequence (3621 bp)
Reference
[1] Whitney JC, Beck CM, Goo YA, et al. Genetically distinct pathways guide effector export through the type VI secretion system. Mol Microbiol. 2014 May;92(3):529-42. doi: 10.1111/mmi.12571. Epub 2014 Mar 28. PMID: 24589350
[2] Zhang H, Zhang H, Gao ZQ, et al. Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein. J Biol Chem. 2013 Feb 22;288(8):5928-39. doi: 10.1074/jbc.M112.434357. Epub 2013 Jan 3. PMID: 23288853
[3] Zhang H, Gao ZQ, Wei Y, et al. Insights into the cross-immunity mechanism within effector families of bacteria type VI secretion system from the structure of StTae4-EcTai4 complex. PLoS One. 2013 Sep 2;8(9):e73782. doi: 10.1371/journal.pone.0073782. eCollection 2013. PMID: 24023903
[4] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[5] Soria-Bustos J, Ares MA, Gómez-Aldapa CA, et al. Two Type VI Secretion Systems of Enterobacter cloacae Are Required for Bacterial Competition, Cell Adherence, and Intestinal Colonization. Front Microbiol. 2020 Sep 24;11:560488. doi: 10.3389/fmicb.2020.560488. eCollection 2020. PMID: 33072020
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ECL_RS07530
Coordinate (Strand) 1555111..1558731 (+)
NCBI ID WP_013096178.1
RefSeq NC_014121
Uniprot ID A0A0H3CHH1_ENTCC
KEGG ID enc:ECL_01536
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 1e-92 211..480
IcmF-related PF06761.14 3.3e-86 532..834
IcmF_C PF06744.14 2.4e-21 1089..1192
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MLTTLLSILTNR
Transmembrane helix 13-34 ILWSFLGVTALSAVIWMIGPLL
Outside 35-48 SIVDTRPLESEQNR
Transmembrane helix 49-71 IISIAVVYLLWAQGHILPRLYNA
Inside 72-471 WLNRKLMDKLNENTAKPDAADPQKRLNSEEQILAGRFDEAAQMLKKAHFSKAGHGAQWTQRFSTQYLYQLPWYVIIGAPGAGKTTALVNSGLQFPLADRFGKTALRGIGGTRNCDWWFTNEAVLLDTAGRYTTQESEQVQDAGEWLEFIGLLRKYRRRQPINGVIITISISDLLTLSPEAARQQAVNLRQRLSELHEQLGIRFPVYVMVTKADLLKGFRAWFADYDKAQRDQIWGFTLPWEQTKHADFDLMGNFHQEFSLLQQRLDAGLPETMLKEHDAKTRAEAYLFPQEFAALRPLLADYLSTVFARSNFETEFSPRGIYFASGTQEGMPFDRVMGELNRALSLPEGAEAEKWDSVSKEAPIPGAKGQSFFIKNLLQNVIFQEAGIAGENRWWELRNR
Transmembrane helix 472-494 AVIWSGYAALLALLVILGGLWLT
Outside 495-1206 SYAKNKAYLEEVDAKVPQLEQQSKALQNQPQRDLFALLPLLNSLVDLPKSEAFDVNDPPVSRRMGLYRGDDVSDASQSLYQKALDQMLLPAVAMHITTWLRNDNGSDVEYSYEALKAYQMLYQPKHYDGKFLHSWVMLNLQRNLPQNVTQAQLQQLEWHLTQLLEPKIQASPYAQDEALVARERALINQQPLSTRVYGRLKRLLEHDENLKPVSLSDLGGPQSELVFSRKSGKPVSEGVPGLYTPDGYWKSFNSQIDSVTTALHEDDAWVLGATTSGEDKQQIDNAVRQLYMRDFIANWDRFLADIQLNNSADLSQRINTARLLSGANSPLRRLVQNLSQVLTLSRNAPAPEDAGKAQDQSNRATRTLEALFRNNDNAPTQGAVVTQAPEQLVTDHYAPMIELAQPLEKGGKTIVFDDFLKQVDELYRYLTAVQDAANSGMPAPGGEAISRLQASAGRLPGGLQTMFSNMAVGASSDTQRRDLENVRKRINVEVGGFCRQAIAGRYPLVRSASTEVTPDDLARMFAPGTGLMDTFFRDNLTNKVDTTQANWRFMPGIDGKTLPGSEGLLRPFQQAQSIRDAFFANGATTPSFKVTVRTVRMDNTILNLTLDVDGQLLRYSHGPQAVQIMNWPGPGGTNQVRMQLGLANGSTATLVTNGAWALNRFFDKARTSPGAGSLSRQATFNVDGHQVTLEFAPNSIRNPFQLPRFSCP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9925 - -


  Protein sequence: 1207 a.a.    

>T6CP004058 NC_014121:1555111-1558731 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssM]
MLTTLLSILTNRILWSFLGVTALSAVIWMIGPLLSIVDTRPLESEQNRIISIAVVYLLWAQGHILPRLYNAWLNRKLMDK
LNENTAKPDAADPQKRLNSEEQILAGRFDEAAQMLKKAHFSKAGHGAQWTQRFSTQYLYQLPWYVIIGAPGAGKTTALVN
SGLQFPLADRFGKTALRGIGGTRNCDWWFTNEAVLLDTAGRYTTQESEQVQDAGEWLEFIGLLRKYRRRQPINGVIITIS
ISDLLTLSPEAARQQAVNLRQRLSELHEQLGIRFPVYVMVTKADLLKGFRAWFADYDKAQRDQIWGFTLPWEQTKHADFD
LMGNFHQEFSLLQQRLDAGLPETMLKEHDAKTRAEAYLFPQEFAALRPLLADYLSTVFARSNFETEFSPRGIYFASGTQE
GMPFDRVMGELNRALSLPEGAEAEKWDSVSKEAPIPGAKGQSFFIKNLLQNVIFQEAGIAGENRWWELRNRAVIWSGYAA
LLALLVILGGLWLTSYAKNKAYLEEVDAKVPQLEQQSKALQNQPQRDLFALLPLLNSLVDLPKSEAFDVNDPPVSRRMGL
YRGDDVSDASQSLYQKALDQMLLPAVAMHITTWLRNDNGSDVEYSYEALKAYQMLYQPKHYDGKFLHSWVMLNLQRNLPQ
NVTQAQLQQLEWHLTQLLEPKIQASPYAQDEALVARERALINQQPLSTRVYGRLKRLLEHDENLKPVSLSDLGGPQSELV
FSRKSGKPVSEGVPGLYTPDGYWKSFNSQIDSVTTALHEDDAWVLGATTSGEDKQQIDNAVRQLYMRDFIANWDRFLADI
QLNNSADLSQRINTARLLSGANSPLRRLVQNLSQVLTLSRNAPAPEDAGKAQDQSNRATRTLEALFRNNDNAPTQGAVVT
QAPEQLVTDHYAPMIELAQPLEKGGKTIVFDDFLKQVDELYRYLTAVQDAANSGMPAPGGEAISRLQASAGRLPGGLQTM
FSNMAVGASSDTQRRDLENVRKRINVEVGGFCRQAIAGRYPLVRSASTEVTPDDLARMFAPGTGLMDTFFRDNLTNKVDT
TQANWRFMPGIDGKTLPGSEGLLRPFQQAQSIRDAFFANGATTPSFKVTVRTVRMDNTILNLTLDVDGQLLRYSHGPQAV
QIMNWPGPGGTNQVRMQLGLANGSTATLVTNGAWALNRFFDKARTSPGAGSLSRQATFNVDGHQVTLEFAPNSIRNPFQL
PRFSCP*

  Nucleotide sequence: 3621 bp    

>T6CP004058 NC_014121:1555111-1558731 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssM]
ATGCTGACGACTCTTCTTTCCATACTGACCAATCGCATTCTGTGGAGCTTCCTGGGCGTTACCGCGCTGTCGGCGGTGAT
CTGGATGATTGGTCCGCTGTTATCCATCGTGGATACCCGCCCGCTCGAATCTGAACAAAACCGCATCATCAGTATTGCGG
TGGTTTATCTGCTCTGGGCGCAGGGGCATATTCTGCCGCGGCTCTACAACGCCTGGCTGAACCGCAAGCTGATGGACAAG
CTCAATGAAAACACCGCCAAACCGGACGCGGCCGATCCCCAGAAGCGGCTGAACAGCGAGGAGCAGATCCTCGCCGGACG
TTTTGATGAAGCGGCGCAAATGCTGAAGAAAGCCCACTTCAGCAAAGCGGGACACGGTGCCCAGTGGACGCAGCGCTTCA
GTACGCAATATCTCTACCAGCTGCCGTGGTACGTCATTATCGGCGCACCGGGCGCCGGTAAAACCACGGCGCTGGTCAAT
TCCGGCCTGCAATTCCCGCTGGCGGATCGCTTCGGTAAAACCGCTCTGCGCGGCATTGGCGGCACCCGCAACTGCGACTG
GTGGTTCACTAATGAAGCCGTCCTGCTGGACACCGCGGGGCGCTACACCACTCAGGAGAGCGAGCAGGTTCAGGATGCCG
GGGAGTGGCTGGAATTTATCGGCCTGCTGCGTAAGTATCGCCGCCGCCAGCCTATTAACGGCGTCATTATCACCATCAGC
ATTTCCGATCTGCTGACGCTCTCCCCGGAAGCGGCGCGCCAGCAGGCGGTTAACCTGCGCCAGCGGCTGTCTGAGCTGCA
TGAACAGCTGGGTATCCGCTTCCCGGTTTACGTGATGGTGACCAAGGCGGACCTGCTTAAAGGCTTCCGCGCCTGGTTTG
CTGACTACGACAAAGCGCAGCGCGACCAGATTTGGGGCTTCACGCTGCCGTGGGAACAGACCAAACACGCCGATTTCGAC
TTGATGGGGAACTTCCACCAGGAGTTCTCGCTGTTGCAGCAGCGTCTGGACGCCGGGCTGCCGGAGACCATGCTGAAAGA
GCATGACGCCAAAACCCGTGCCGAAGCGTATCTCTTCCCGCAGGAGTTCGCGGCGCTGCGCCCGCTGCTGGCAGACTACC
TGAGCACGGTCTTTGCCCGCTCTAACTTTGAGACCGAGTTCTCCCCACGCGGAATATACTTCGCCAGCGGCACCCAGGAA
GGCATGCCGTTCGACCGCGTCATGGGTGAACTGAACCGCGCCCTGTCACTGCCGGAAGGGGCAGAGGCAGAGAAGTGGGA
TTCGGTCAGTAAAGAGGCGCCGATCCCCGGTGCAAAAGGGCAGAGCTTCTTCATTAAAAACCTGCTGCAAAACGTCATTT
TCCAGGAAGCCGGTATTGCCGGTGAAAACCGCTGGTGGGAACTGCGCAACCGCGCGGTGATCTGGTCCGGATACGCGGCG
CTGCTGGCGCTGCTGGTGATCCTTGGCGGACTGTGGCTGACCAGCTATGCCAAAAATAAGGCCTATCTGGAAGAGGTGGA
CGCTAAAGTCCCGCAGCTGGAGCAGCAGAGTAAAGCGTTACAGAATCAGCCCCAGCGCGATCTGTTCGCGCTCCTGCCGC
TGCTGAACAGCCTGGTCGATCTGCCGAAAAGCGAGGCATTCGATGTCAACGACCCGCCGGTCTCCCGCCGTATGGGGCTC
TATCGCGGGGATGACGTCAGCGATGCTTCCCAGTCGCTGTATCAGAAAGCGCTGGATCAGATGCTGCTGCCCGCCGTGGC
GATGCATATCACCACCTGGCTGCGCAACGACAACGGCAGCGACGTGGAATACAGCTACGAGGCGCTGAAAGCCTACCAGA
TGCTGTATCAGCCTAAACACTACGACGGCAAATTCCTGCATTCCTGGGTGATGCTCAATTTACAGCGTAATCTGCCGCAG
AACGTGACGCAGGCGCAGCTGCAACAGCTGGAGTGGCACCTGACGCAGCTGCTGGAGCCAAAAATTCAGGCTTCGCCGTA
TGCCCAGGACGAAGCGCTGGTGGCTCGCGAGAGGGCGCTCATCAACCAGCAGCCGCTCTCCACCCGCGTGTATGGGCGTC
TGAAGCGTCTGCTGGAGCATGACGAAAACCTGAAGCCGGTCTCCCTGTCCGACCTGGGTGGCCCGCAGAGCGAACTGGTG
TTCTCCCGCAAAAGCGGCAAACCGGTGAGCGAAGGCGTGCCGGGGCTCTACACCCCGGACGGCTACTGGAAGAGCTTTAA
CAGCCAGATTGACAGCGTGACCACGGCGCTGCACGAAGATGATGCCTGGGTGCTGGGCGCGACCACCTCCGGGGAAGACA
AGCAGCAGATCGATAACGCCGTGCGTCAGCTCTACATGCGCGACTTTATCGCAAACTGGGATCGTTTCCTCGCCGACATT
CAGCTTAACAACAGCGCCGATCTCTCGCAGCGCATCAACACGGCGCGCCTGCTCTCCGGCGCAAACTCGCCGCTGCGCCG
TCTGGTGCAGAACCTGAGCCAGGTGCTGACGCTGTCGCGCAACGCGCCAGCGCCGGAGGACGCGGGGAAAGCGCAAGACC
AGAGTAACCGCGCCACCCGTACCCTCGAAGCGCTGTTCCGCAATAACGACAATGCTCCGACGCAGGGGGCAGTAGTGACG
CAGGCACCTGAACAGCTGGTGACCGACCATTACGCGCCAATGATCGAGCTGGCTCAGCCGCTGGAGAAGGGCGGCAAGAC
CATCGTCTTTGATGATTTCCTCAAGCAGGTGGATGAGCTGTATCGTTATCTGACCGCGGTGCAGGACGCTGCCAACAGCG
GCATGCCAGCCCCGGGTGGTGAAGCAATCAGCCGTCTGCAGGCCAGCGCCGGGCGTCTGCCGGGCGGGCTGCAAACCATG
TTCAGCAACATGGCGGTGGGGGCCAGCAGTGATACCCAGCGTCGCGATCTGGAAAACGTCCGTAAGCGCATTAACGTCGA
AGTGGGTGGTTTCTGCCGTCAGGCAATTGCCGGTCGCTACCCGCTGGTGCGCAGCGCCAGCACCGAGGTGACGCCGGACG
ATCTGGCACGCATGTTTGCGCCGGGTACCGGGCTGATGGATACCTTCTTTCGCGACAACCTGACCAACAAAGTGGATACC
ACTCAGGCGAACTGGCGCTTTATGCCGGGGATCGACGGTAAAACGCTGCCGGGAAGTGAAGGACTGCTGCGACCGTTCCA
GCAGGCCCAGTCCATTCGCGATGCCTTCTTTGCTAACGGTGCGACGACCCCCTCTTTCAAGGTGACGGTGCGTACCGTGC
GGATGGATAACACCATTCTTAACCTGACGCTGGACGTTGACGGCCAACTGCTGCGTTACAGCCACGGTCCACAGGCGGTG
CAGATCATGAACTGGCCAGGGCCGGGCGGCACCAACCAGGTGCGTATGCAGCTGGGTCTGGCCAACGGCAGCACCGCGAC
GCTGGTAACCAACGGCGCCTGGGCCCTGAACCGCTTCTTCGATAAAGCGCGCACCAGCCCTGGGGCAGGTAGTCTGAGCC
GTCAGGCCACGTTCAACGTCGATGGCCATCAGGTCACGCTGGAGTTTGCGCCGAACAGCATTCGCAATCCGTTCCAGCTT
CCTCGTTTCTCATGCCCATAA