Detailed information of component protein


Summary

Component ID T6CP004055
Component protein type TssJ
T6SS ID (Type) T6SS00395 experimental (Type i3)
Strain Enterobacter cloacae subsp. cloacae ATCC 13047
Replicon chromosome
Sequence Protein sequence (168 a.a.); Nucleotide sequence (504 bp)
Reference
[1] Whitney JC, Beck CM, Goo YA, et al. Genetically distinct pathways guide effector export through the type VI secretion system. Mol Microbiol. 2014 May;92(3):529-42. doi: 10.1111/mmi.12571. Epub 2014 Mar 28. PMID: 24589350
[2] Zhang H, Zhang H, Gao ZQ, et al. Structure of the type VI effector-immunity complex (Tae4-Tai4) provides novel insights into the inhibition mechanism of the effector by its immunity protein. J Biol Chem. 2013 Feb 22;288(8):5928-39. doi: 10.1074/jbc.M112.434357. Epub 2013 Jan 3. PMID: 23288853
[3] Zhang H, Gao ZQ, Wei Y, et al. Insights into the cross-immunity mechanism within effector families of bacteria type VI secretion system from the structure of StTae4-EcTai4 complex. PLoS One. 2013 Sep 2;8(9):e73782. doi: 10.1371/journal.pone.0073782. eCollection 2013. PMID: 24023903
[4] Russell AB, Singh P, Brittnacher M, et al. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host Microbe. 2012 May 17;11(5):538-49. doi: 10.1016/j.chom.2012.04.007. PMID: 22607806
[5] Soria-Bustos J, Ares MA, Gómez-Aldapa CA, et al. Two Type VI Secretion Systems of Enterobacter cloacae Are Required for Bacterial Competition, Cell Adherence, and Intestinal Colonization. Front Microbiol. 2020 Sep 24;11:560488. doi: 10.3389/fmicb.2020.560488. eCollection 2020. PMID: 33072020
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ECL_RS07515
Coordinate (Strand) 1551980..1552483 (+)
NCBI ID WP_013096175.1
RefSeq NC_014121
Uniprot ID A0A5E1AD78_9ENTR, A0A1B3F244_ENTCL, A0A331GQD1_KLEPN, A0A0H3CIW8_ENTCC
KEGG ID enc:ECL_01533, eclg:EC036_26100
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 7.5e-25 34..137
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-9 MNNKNFHKL
Transmembrane helix 10-29 WFVFYAVIFALVSGCSSSSH
Inside 30-167 SDPSRYNLQFQAHPQINDSAPLKVRVLLLKSDADFMSSDFYSLQNNASATLGANLLNSDVFFLMPGQLSKTLSGQSSPEARYIGVMAEYQMLDGKKWRVSLPLPVPGENHLYQFWKWSSDELQANVFLDINGIRVISQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9981 pos: 23-24. VSG-CS. MNNKNFHKLWFVFYAVIFALVSG


  Protein sequence: 168 a.a.    

>T6CP004055 NC_014121:1551980-1552483 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssJ]
MNNKNFHKLWFVFYAVIFALVSGCSSSSHSDPSRYNLQFQAHPQINDSAPLKVRVLLLKSDADFMSSDFYSLQNNASATL
GANLLNSDVFFLMPGQLSKTLSGQSSPEARYIGVMAEYQMLDGKKWRVSLPLPVPGENHLYQFWKWSSDELQANVFLDIN
GIRVISQ*

  Nucleotide sequence: 504 bp    

>T6CP004055 NC_014121:1551980-1552483 [Enterobacter cloacae subsp. cloacae ATCC 13047] [TssJ]
ATGAATAATAAAAATTTTCATAAGCTGTGGTTCGTCTTTTACGCGGTGATATTCGCCCTGGTCAGTGGTTGTTCGTCGTC
TTCACACAGCGACCCCTCCCGCTACAATCTGCAGTTTCAGGCTCATCCACAAATCAATGATTCTGCGCCGCTTAAAGTCA
GAGTGCTGCTGTTGAAGTCCGATGCGGATTTCATGTCCAGTGACTTCTATTCCCTGCAGAACAACGCGTCAGCCACGCTT
GGCGCGAATCTGCTGAACAGCGATGTTTTCTTCCTGATGCCGGGCCAGCTTTCGAAAACCCTTAGCGGACAAAGCTCACC
CGAGGCGCGCTATATCGGTGTTATGGCCGAATATCAGATGCTGGATGGAAAGAAATGGCGTGTCTCACTCCCACTGCCTG
TTCCGGGCGAAAACCATCTCTACCAGTTCTGGAAATGGTCATCCGATGAACTCCAGGCCAATGTCTTTCTCGACATAAAT
GGCATCCGGGTCATCAGCCAGTAA