SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00375
Cognate effector	EFF01872
Related T6SS (Type)	T6SS00395 (Type i3)
Strain	Enterobacter cloacae subsp. cloacae ATCC 13047
Replicon	chromosome
Sequence	Protein sequence (254 a.a.); Nucleotide sequence (762 bp)
Description	Wild-type cells outcompeting Δtle Δtli mutants almost 103-fold . Competitive fitness is restored to these latter targets when complemented with the wild-type tli gene (Fig.
Reference	[1] Donato SL, Beck CM, Garza-Sánchez F, et al. The β-encapsulation cage of rearrangement hotspot (Rhs) effectors is required for type VI secretion. Proc Natl Acad Sci U S A. 2020 Dec 15. pii: 201919350. doi: 10.1073/pnas.1919350117. [Epub ahead of print] PMID: 33323487

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	ECL_01554 (Tli)
Coordinates (Strand)	1577823..1578584 (+)
NCBI ID	296101916
RefSeq	NC_014121
Uniprot ID	A0A0H3CKN4_ENTCC
KEGG ID	enc:ECL_01554
PDB ID	7KB2

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Ank_2	PF12796.9	7.8e-06	123..205

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-253	MKSFLSGWIMLCLILMVQGCKQNMDLKPDNYFSGQQLTLARAIENGEVDEVIKLASGTDLNKPGKEDMTLLFWAVMNSINNQKTPERLNVITMLIKAGADPLQPRPQGKNSPAEFVLMADNADWIKAMLNAGLSPNAVDKTFGKPIIFQTLEAKNTKTLQAMLDKGADINITDSLGNTLLIDALDFHSYDHVLLLLERGADPEIKADNGWTMGNQLQRFLDRAKVGSDEYKKLNEIKDVLIQHGGKWPPTPVK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9950	pos: 19-20. VQG-CK.	MKSFLSGWIMLCLILMVQG

Protein sequence: 254 a.a. .

                                    >IMU00375 NC_014121:1577823-1578584 [Enterobacter cloacae subsp. cloacae ATCC 13047]
MKSFLSGWIMLCLILMVQGCKQNMDLKPDNYFSGQQLTLARAIENGEVDEVIKLASGTDLNKPGKEDMTLLFWAVMNSIN
NQKTPERLNVITMLIKAGADPLQPRPQGKNSPAEFVLMADNADWIKAMLNAGLSPNAVDKTFGKPIIFQTLEAKNTKTLQ
AMLDKGADINITDSLGNTLLIDALDFHSYDHVLLLLERGADPEIKADNGWTMGNQLQRFLDRAKVGSDEYKKLNEIKDVL
IQHGGKWPPTPVK*

                                

Nucleotide sequence: 762 bp .

                                    >IMU00375 NC_014121:1577823-1578584 [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGAAATCGTTCTTATCAGGCTGGATAATGCTTTGTTTGATTTTAATGGTTCAGGGGTGTAAACAAAATATGGATTTAAA
ACCAGATAATTACTTTAGCGGACAACAGCTCACATTAGCTCGTGCAATCGAGAATGGCGAAGTTGATGAGGTAATTAAAT
TAGCGTCAGGGACGGATTTAAACAAGCCCGGTAAAGAAGATATGACATTGTTATTTTGGGCTGTCATGAATTCGATCAAT
AATCAGAAAACACCGGAGCGGTTAAATGTTATCACCATGCTGATTAAAGCGGGTGCAGACCCTCTGCAACCGAGGCCACA
AGGAAAAAATAGTCCAGCTGAGTTTGTTTTAATGGCAGACAACGCGGACTGGATCAAGGCGATGTTAAATGCGGGTTTAT
CGCCAAATGCCGTTGATAAAACTTTTGGGAAACCAATTATTTTCCAAACACTTGAAGCCAAAAACACCAAGACGTTGCAG
GCTATGCTTGATAAAGGTGCCGATATAAATATCACCGATTCTTTGGGAAATACACTACTTATTGATGCCCTGGATTTCCA
CTCGTACGATCATGTTCTTCTTTTACTTGAACGCGGGGCTGACCCTGAAATCAAAGCTGATAATGGTTGGACGATGGGTA
ATCAGTTGCAGCGTTTCCTCGATAGGGCGAAGGTAGGCAGCGACGAATATAAAAAACTTAACGAAATTAAAGATGTGCTT
ATTCAACATGGCGGAAAATGGCCACCAACTCCGGTTAAATAG

                                