Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000043  |
| Strain | Escherichia coli str. K-12 substr. MC4100 strain K-12 |
| Replicon | chromosome |
| Sequence | Protein sequence (363 a.a.); Nucleotide sequence (1089 bp) |
| Response to T6SS attack | protective |
| Known or putative function | outer membrane stability |
| Description | The ΔompCΔompF double mutant strain and the ΔompT derivative stood out as particularly sensitive to the killing by V. cholerae. |
| Reference |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | BN896_RS04350 (ompF) |
| Coordinates (Strand) | 888085..889173 (-) |
| NCBI ID | 446900664 |
| RefSeq | NZ_HG738867 |
| Uniprot ID | A0A0E0Y309_ECO1C, K4WYC7_ECOLX, U9XEN3_ECOLX, E3PIV0_ECOH1, A7ZK20_ECO24, B6I901_ECOSE, A0A140NAN5_ECOBD, J7QZQ9_ECOLX, E0IZB3_ECOLW, B7LE29_ECO55, A0A1X3JJZ0_ECOLX, A0A1X3L4S5_ECOLX, F4VCV3_ECOLX, F4SM43_ECOLX, E1IQ54_ECOLX, D8EAJ3_ECOLX, A0A0E1LVK6_ECOLX, E9TEP5_ECOLX, I4SAG3_ECOLX, OMPF_ECOLI, I2SYG0_ECOLX, I2WW12_ECOLX |
| KEGG ID | elw:ECW_m1039, ecw:EcE24377A_1029, ebd:ECBD_2666, ecj:JW0912, ecy:ECSE_0988, eco:b0929, eck:EC55989_0978, esl:O3K_16695, elh:ETEC_0997 |
| PDB ID | 1BT9, 1GFM, 1GFN, 1GFO, 1GFP, 1GFQ, 1HXT, 1HXU, 1HXX, 1MPF, 1OPF, 2OMF, 2ZFG, 2ZLD, 3FYX, 3HW9, 3HWB, 3K19, 3K1B, 3O0E, 3POQ, 3POU, 3POX, 4D5U, 4GCP, 4GCQ, 4GCS, 4JFB, 4LSE, 4LSF, 4LSH, 4LSI, 5NUO, 5NUQ, 5NUR, 6WTZ, 6ZHP, 6ZHV, 7NST, 7NSU |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Porin_1 | PF00267.23 | 1.9e-139 | 28..362 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-362 | MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHYFSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNKTRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVATYRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADRTNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKTQDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQIDSDNKLGVGSDDTVAVGIVYQF |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9989 | pos: 22-23. ANA-AE. | MMKRNILAVIVPALLVAGTANA |
Protein sequence: 363 a.a. .
>RSF000043 NZ_HG738867:c889173-888085 [Escherichia coli str. K-12 substr. MC4100]
MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHYFSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGY
GQWEYNFQGNNSEGADAQTGNKTRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADRTNLQEAQPLGNGKKAEQWATGL
KYDANNIYLAANYGETRNATPITNKFTNTSGFANKTQDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVG
ATYYFNKNMSTYVDYIINQIDSDNKLGVGSDDTVAVGIVYQF*
MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHYFSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGY
GQWEYNFQGNNSEGADAQTGNKTRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADRTNLQEAQPLGNGKKAEQWATGL
KYDANNIYLAANYGETRNATPITNKFTNTSGFANKTQDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVG
ATYYFNKNMSTYVDYIINQIDSDNKLGVGSDDTVAVGIVYQF*
Nucleotide sequence: 1089 bp .
>RSF000043 NZ_HG738867:c889173-888085 [Escherichia coli str. K-12 substr. MC4100]
ATGATGAAGCGCAATATTCTGGCAGTGATCGTCCCTGCTCTGTTAGTAGCAGGTACTGCAAACGCTGCAGAAATCTATAA
CAAAGATGGCAACAAAGTAGATCTGTACGGTAAAGCTGTTGGTCTGCATTATTTTTCCAAGGGTAACGGTGAAAACAGTT
ACGGTGGCAATGGCGACATGACCTATGCCCGTCTTGGTTTTAAAGGGGAAACTCAAATCAATTCCGATCTGACCGGTTAT
GGTCAGTGGGAATATAACTTCCAGGGTAACAACTCTGAAGGCGCTGACGCTCAAACTGGTAACAAAACGCGTCTGGCATT
CGCGGGTCTTAAATACGCTGACGTTGGTTCTTTCGATTACGGCCGTAACTACGGTGTGGTTTATGATGCACTGGGTTACA
CCGATATGCTGCCAGAATTTGGTGGTGATACTGCATACAGCGATGACTTCTTCGTTGGTCGTGTTGGCGGCGTTGCTACC
TATCGTAACTCCAACTTCTTTGGTCTGGTTGATGGCCTGAACTTCGCTGTTCAGTACCTGGGTAAAAACGAGCGTGACAC
TGCACGCCGTTCTAACGGCGACGGTGTTGGCGGTTCTATCAGCTACGAATACGAAGGCTTTGGTATCGTTGGTGCTTATG
GTGCAGCTGACCGTACCAACCTGCAAGAAGCTCAACCTCTTGGCAACGGTAAAAAAGCTGAACAGTGGGCTACTGGTCTG
AAGTACGACGCGAACAACATCTACCTGGCAGCGAACTACGGTGAAACCCGTAACGCTACGCCGATCACTAATAAATTTAC
AAACACCAGCGGCTTCGCCAACAAAACGCAAGACGTTCTGTTAGTTGCGCAATACCAGTTCGATTTCGGTCTGCGTCCGT
CCATCGCTTACACCAAATCTAAAGCGAAAGACGTAGAAGGTATCGGTGATGTTGATCTGGTGAACTACTTTGAAGTGGGC
GCAACCTACTACTTCAACAAAAACATGTCCACCTATGTTGACTACATCATCAACCAGATCGATTCTGACAACAAACTGGG
CGTAGGTTCAGACGACACCGTTGCTGTGGGTATCGTTTACCAGTTCTAA
ATGATGAAGCGCAATATTCTGGCAGTGATCGTCCCTGCTCTGTTAGTAGCAGGTACTGCAAACGCTGCAGAAATCTATAA
CAAAGATGGCAACAAAGTAGATCTGTACGGTAAAGCTGTTGGTCTGCATTATTTTTCCAAGGGTAACGGTGAAAACAGTT
ACGGTGGCAATGGCGACATGACCTATGCCCGTCTTGGTTTTAAAGGGGAAACTCAAATCAATTCCGATCTGACCGGTTAT
GGTCAGTGGGAATATAACTTCCAGGGTAACAACTCTGAAGGCGCTGACGCTCAAACTGGTAACAAAACGCGTCTGGCATT
CGCGGGTCTTAAATACGCTGACGTTGGTTCTTTCGATTACGGCCGTAACTACGGTGTGGTTTATGATGCACTGGGTTACA
CCGATATGCTGCCAGAATTTGGTGGTGATACTGCATACAGCGATGACTTCTTCGTTGGTCGTGTTGGCGGCGTTGCTACC
TATCGTAACTCCAACTTCTTTGGTCTGGTTGATGGCCTGAACTTCGCTGTTCAGTACCTGGGTAAAAACGAGCGTGACAC
TGCACGCCGTTCTAACGGCGACGGTGTTGGCGGTTCTATCAGCTACGAATACGAAGGCTTTGGTATCGTTGGTGCTTATG
GTGCAGCTGACCGTACCAACCTGCAAGAAGCTCAACCTCTTGGCAACGGTAAAAAAGCTGAACAGTGGGCTACTGGTCTG
AAGTACGACGCGAACAACATCTACCTGGCAGCGAACTACGGTGAAACCCGTAACGCTACGCCGATCACTAATAAATTTAC
AAACACCAGCGGCTTCGCCAACAAAACGCAAGACGTTCTGTTAGTTGCGCAATACCAGTTCGATTTCGGTCTGCGTCCGT
CCATCGCTTACACCAAATCTAAAGCGAAAGACGTAGAAGGTATCGGTGATGTTGATCTGGTGAACTACTTTGAAGTGGGC
GCAACCTACTACTTCAACAAAAACATGTCCACCTATGTTGACTACATCATCAACCAGATCGATTCTGACAACAAACTGGG
CGTAGGTTCAGACGACACCGTTGCTGTGGGTATCGTTTACCAGTTCTAA