Detailed information of Recipient susceptibility factor


Summary

ID RSF000058 experimental
Strain Klebsiella variicola
Replicon chromosome
Sequence Protein sequence (378 a.a.); Nucleotide sequence (1134 bp)
Response to T6SS attack protective
Known or putative function capsule protection
Description Group I capsules confer a strong protection from V. cholerae's T6SS attacks to commensal Klebsiella strains.
Reference
experimental Experimental investigation has been performed.
External database links

Locus tag (Gene) KPK_RS07975 (wza)
Coordinates (Strand) 1699259..1700392 (+)
NCBI ID WP_012541078.1
RefSeq NC_011283
Uniprot ID UPI00018136D9
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Poly_export PF02563.18 6.6e-28 82..165
Wza_C PF18412.3 6.7e-18 345..374
SLBB PF10531.11 8.3e-08 173..222
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MKKK
Transmembrane helix 5-27 LVRFSALALAISFLSGCTIIPGQ
Outside 28-377 GLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPETVLARPNPQLDNLLRSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGRVQVAGKTLTQVRQEIASRLTTYIESPQVDVSIAAFRSQKTYVTGEVMKSGQQPITNIPLTVMDAINAAGGLAPDADWRNVVLTHNGKDTKISLYALMQKGDLSQNHLLYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGMTLAEAIGNAEGMTQAFSDATGVFVIRQLKNDKQGKIANIYQLNAQDASAMVLGTEFQLQPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETTRYIKNW


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9983 pos: 20-21. LSG-CT. MKKKLVRFSALALAISFLSG


  Protein sequence: 378 a.a.    .

>RSF000058 NC_011283:1699259-1700392 [Klebsiella variicola]
MKKKLVRFSALALAISFLSGCTIIPGQGLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPETVLARPNPQLDNLL
RSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGRVQVAGKTLTQVRQEIASRLTTYIESPQ
VDVSIAAFRSQKTYVTGEVMKSGQQPITNIPLTVMDAINAAGGLAPDADWRNVVLTHNGKDTKISLYALMQKGDLSQNHL
LYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGMTLAEAIGNAEGMTQAFSDATGVFVIRQLKNDKQGKIANIYQLNA
QDASAMVLGTEFQLQPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETTRYIKNW*

  Nucleotide sequence: 1134 bp    .

>RSF000058 NC_011283:1699259-1700392 [Klebsiella variicola]
ATGAAGAAAAAACTTGTTAGATTTTCGGCATTAGCGTTGGCGATTAGTTTTTTATCAGGCTGTACTATTATTCCTGGTCA
AGGATTAAATAGTCTACGCAAAAATGTGGTCGAACTACCGGACAGCGATTACGATCTGGATAAGTTGGTCAATGTATACC
CAATGACACCGGGGCTTATCGATCAGCTCCGTCCGGAGACTGTTCTGGCGAGACCCAACCCGCAGCTGGATAATTTGCTT
CGAAGTTACGAATATCGCATTGGGGTCGGCGACGTATTGATGGTTACCGTATGGGATCATCCAGAACTGACAACCCCTGC
GGGCCAATACCGCAGTGCTAGCGATACAGGTAACTGGGTTAATTCTGATGGCACAATCTTTTACCCTTATATAGGCAGGG
TTCAGGTTGCCGGCAAAACTCTTACCCAGGTACGCCAGGAAATAGCTAGTCGTTTAACTACTTATATCGAAAGTCCTCAG
GTTGATGTTAGCATTGCAGCTTTTAGATCGCAGAAAACATATGTTACTGGAGAGGTAATGAAATCGGGCCAACAACCGAT
AACGAATATTCCTTTAACGGTAATGGATGCCATAAATGCCGCTGGAGGTCTTGCTCCAGATGCTGATTGGCGCAATGTTG
TGTTAACTCATAATGGTAAGGATACAAAGATATCCTTATATGCGCTGATGCAAAAAGGAGACTTGTCACAAAATCATTTA
CTGTATCCGGGTGATATATTATTTGTCCCACGAAACGATGATTTAAAAGTATTTGTAATGGGAGAAGTGGGCAAACAAAG
TACCATGAAAATGGATCGAAGTGGGATGACTTTAGCAGAAGCGATTGGCAATGCCGAAGGTATGACGCAAGCTTTTAGCG
ATGCCACTGGGGTATTTGTTATAAGACAACTGAAAAATGACAAGCAGGGTAAAATAGCTAATATATATCAGCTTAATGCC
CAAGATGCTTCAGCGATGGTGCTGGGTACTGAGTTTCAGTTGCAGCCTTATGATATTGTCTATGTCACTACTGCTCCACT
TGTAAGGTGGAATCGTGTTATATCTCAGCTAGTACCTACAATCACAGGCGTCCATGATATGACAGAAACTACACGCTATA
TTAAAAATTGGTGA