Detailed information of immunity protein


Summary

Immunity protein ID IMU00349 experimental
Cognate effector EFF01826 experimental
Related T6SS (Type) T6SS01216 experimental (Type Unknown)
Strain Klebsiella pneumoniae strain Kp52.145
Replicon chromosome
Sequence Protein sequence (425 a.a.); Nucleotide sequence (1275 bp)
Description Induction of Sel1E abrogated VgrG4-dependent killing and Sel1E conferred protein against the toxicity triggered by VgrG4570-899 and VgrG4612-759 containing the DUF2345 domain results that Sel1E is the immunity protein that protects against the toxic activity of VgrG4
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) BN49_RS18785 (Sel1E)
Coordinates (Strand) 3496153..3497427 (-)
NCBI ID 802146974
RefSeq NZ_FO834906
Uniprot ID UPI0005FC2A3E
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF6396 PF19933.1 5.6e-47 231..338
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-424 MRQLILFLSLMACLLHSASSAKKDLFVNPLSDTHAALAFTCAHQIIPEASADTDVLFKYARWLQKNNLLKQDKSVDAQTERLYRIAAENGHYKASINLQNGALRGQFSLSSHEQFRLSQQLIAAGVATGYYLTAIYLERGVAGLQQDPALALRYYRKAADEGNPQAQAYVGDKLAPVDRAPDIAHQMRRCAAEQGEGKAAVMLGVNLQGKGYYRRAIEAFQLGVAAGDTSSALALSHGFDGPESSDELYYLAQQKDPERARRYKLITKILSNYSYASPTVPEINDIVPLPPAPLPEWDGKLKWLEEWEANIPPPAPDAALIEKLAQAKQLNPATGRPLPTSPDFEKDSVARLQCRSGEPCPQSGYWQPAWRPREGMSEHAIRYFREGDIMPVEKVTFVRPRPWPLRDRLVVEAQETVWRRVSEA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.5355 pos: 21-22. SSA-KK. MRQLILFLSLMACLLHSASSA


  Protein sequence: 425 a.a.    .

>IMU00349 NZ_FO834906:c3497427-3496153 [Klebsiella pneumoniae]
MRQLILFLSLMACLLHSASSAKKDLFVNPLSDTHAALAFTCAHQIIPEASADTDVLFKYARWLQKNNLLKQDKSVDAQTE
RLYRIAAENGHYKASINLQNGALRGQFSLSSHEQFRLSQQLIAAGVATGYYLTAIYLERGVAGLQQDPALALRYYRKAAD
EGNPQAQAYVGDKLAPVDRAPDIAHQMRRCAAEQGEGKAAVMLGVNLQGKGYYRRAIEAFQLGVAAGDTSSALALSHGFD
GPESSDELYYLAQQKDPERARRYKLITKILSNYSYASPTVPEINDIVPLPPAPLPEWDGKLKWLEEWEANIPPPAPDAAL
IEKLAQAKQLNPATGRPLPTSPDFEKDSVARLQCRSGEPCPQSGYWQPAWRPREGMSEHAIRYFREGDIMPVEKVTFVRP
RPWPLRDRLVVEAQETVWRRVSEA*

  Nucleotide sequence: 1275 bp    .

>IMU00349 NZ_FO834906:c3497427-3496153 [Klebsiella pneumoniae]
ATGCGCCAGTTAATATTATTCCTGTCGCTGATGGCATGTCTCCTGCACAGCGCATCGTCTGCTAAAAAGGACTTATTTGT
GAATCCTCTTTCCGATACTCACGCCGCCCTGGCGTTTACCTGCGCACATCAGATAATTCCTGAGGCGTCTGCTGATACCG
ATGTCCTGTTTAAATACGCCCGCTGGCTGCAGAAAAATAACCTGCTGAAGCAGGATAAAAGCGTCGATGCGCAAACCGAA
CGGCTGTACCGGATAGCGGCTGAGAACGGGCACTATAAGGCCAGCATTAATCTGCAGAACGGCGCCCTGCGCGGGCAGTT
TTCGCTGAGCAGCCATGAGCAGTTCCGGCTGAGCCAGCAGCTTATCGCTGCCGGGGTGGCGACCGGGTACTATCTGACCG
CTATCTATCTGGAACGGGGCGTCGCGGGGCTGCAGCAGGATCCGGCGCTTGCCCTGCGCTATTACCGCAAGGCGGCGGAT
GAGGGCAATCCGCAGGCGCAGGCATATGTGGGGGATAAGCTGGCGCCGGTGGACAGGGCGCCCGATATTGCACACCAGAT
GCGTCGTTGCGCCGCGGAACAGGGAGAGGGAAAGGCGGCGGTAATGCTGGGGGTTAACTTACAGGGAAAAGGTTATTATC
GGAGAGCAATTGAAGCTTTCCAGTTAGGTGTGGCGGCTGGAGACACTTCCTCAGCGCTTGCTTTAAGTCATGGGTTTGAT
GGCCCCGAATCTTCAGATGAACTTTATTATCTGGCTCAGCAGAAGGATCCGGAACGTGCCCGGCGCTATAAACTGATAAC
GAAGATCCTGAGCAATTATTCCTACGCCAGCCCCACGGTTCCGGAAATCAACGATATCGTGCCGCTGCCGCCGGCGCCGC
TGCCGGAATGGGACGGCAAGCTGAAGTGGCTGGAGGAATGGGAGGCCAATATCCCGCCGCCAGCGCCAGATGCGGCGCTG
ATAGAGAAACTGGCGCAGGCGAAACAGCTGAACCCGGCGACGGGCAGGCCGCTGCCAACGTCACCGGACTTTGAGAAGGA
CAGCGTGGCGCGGCTGCAGTGCCGCAGCGGTGAGCCCTGCCCGCAAAGCGGCTACTGGCAGCCGGCGTGGCGGCCCCGTG
AAGGGATGAGCGAACATGCCATCCGTTACTTCCGCGAGGGGGACATCATGCCGGTGGAGAAGGTGACCTTCGTTCGGCCA
CGTCCCTGGCCGCTGCGTGACAGACTGGTCGTGGAGGCGCAGGAGACGGTGTGGCGCCGGGTCAGCGAGGCATAA