Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS01216 experimental
T6SS type Type Unknown
Strain Klebsiella pneumoniae strain Kp52.145
Replicon (RefSeq) chromosome (NZ_FO834906)
Location 3477664..3505840
Function anti-bacterial; anti-eukaryotic
Comment Kp52145 T6SS-dependent intra and inter species bacterial competition is dependent on VgrG4 and GST-VgrG4 caused a strong inhibition of yeast growth show VgrG4 is a bona fide T6SS toxin and that the C-terminal extension containing the DUF2345 domain is sufficient for the anti-bacterial and anti-eukaryotic effect
References
[1] Storey D, McNally A, strand M, Sa-Pessoa Graca Santos J, Rodriguez-Escudero I, Elmore B, Palacios L, Marshall H, Hobley L, Molina M, Cid VJ, Salminen TA, Bengoechea JA. et al (2020) Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent.. PLoS Pathog. 16(3):e1007969. [PudMed:32191774]
experimental Experimental investigation has been performed.
T6SS components and genome coordinates

ComponentTssATssETssFTssGTssITssJTssKTssL
Number11111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
BN49_RS18685 3472745..3473791 - 1047 WP_004175547.1 MerR family transcriptional regulator
BN49_RS18690 (ychH) 3474012..3474287 - 276 WP_002910437.1 stress-induced protein YchH
BN49_RS18695 (pth) 3474560..3475144 + 585 WP_004180389.1 aminoacyl-tRNA hydrolase
BN49_RS18700 (ychF) 3475262..3476353 + 1092 WP_002910443.1 redox-regulated ATPase YchF
BN49_RS18705 3476436..3476765 - 330 WP_016530916.1 DUF1869 domain-containing protein
BN49_RS18710 3476850..3477532 - 683 pseudogene substrate-binding domain-containing protein
T6CP015378 TssA BN49_RS18715 3477664..3479079 - 1416 WP_046043538.1 type VI secretion system ImpA family N-terminal domain-containing protein
T6CP015379 TssE BN49_RS18720 (tssE) 3479099..3479542 - 444 WP_004175538.1 type VI secretion system baseplate subunit TssE
T6CP015380 TssJ BN49_RS18725 (tssJ) 3479545..3480081 - 537 WP_077255016.1 type VI secretion system lipoprotein TssJ
T6CP015381 TssG BN49_RS18730 (tssG) 3480062..3481078 - 1017 WP_164995970.1 type VI secretion system baseplate subunit TssG
T6CP015382 TssF BN49_RS18735 (tssF) 3481108..3482871 - 1764 WP_046043540.1 type VI secretion system baseplate subunit TssF
BN49_RS18740 3483005..3486395 - 3391 pseudogene type VI secretion protein VasK
BN49_RS18745 3486501..3487646 - 1146 WP_016532067.1 hypothetical protein
BN49_RS18750 3487766..3488029 - 264 WP_016532068.1 PAAR domain-containing protein
BN49_RS18755 3488119..3489096 - 978 WP_016532069.1 hypothetical protein
BN49_RS18760 3489311..3490366 - 1056 WP_129015098.1 sel1 repeat family protein
BN49_RS18765 3490413..3491693 - 1281 WP_046043544.1 sel1 repeat family protein
BN49_RS18770 3491731..3493002 - 1272 WP_038431327.1 sel1 repeat family protein
BN49_RS18775 3493002..3494873 - 1872 WP_046043546.1 phospholipase
BN49_RS18780 3495042..3496115 - 1074 WP_040148336.1 sel1 repeat family protein
BN49_RS18785 3496153..3497427 - 1275 WP_046043550.1 sel1 repeat family protein
BN49_RS30500 3497427..3498994 - 1568 pseudogene phosphatidylserine/phosphatidylglycerophosphate/ cardiolipin synthase family protein
T6CP015383 TssI BN49_RS18800 (vgrG) 3498991..3501690 - 2700 WP_046043552.1 type VI secretion system tip protein VgrG
BN49_RS31145 3501841..3501965 - 125 pseudogene hypothetical protein
BN49_RS18805 3502149..3503846 - 1698 WP_032102707.1 OmpA family protein
T6CP015384 TssL BN49_RS18810 3503850..3504503 - 654 WP_004891113.1 type VI secretion system protein TssL, short form
T6CP015385 TssK BN49_RS18815 (tssK) 3504500..3505840 - 1341 WP_032102706.1 type VI secretion system baseplate subunit TssK
BN49_RS18825 3506409..3506738 + 330 WP_002910650.1 gamma-glutamylcyclotransferase
BN49_RS18830 3506853..3507392 + 540 WP_002910652.1 DUF2058 domain-containing protein
BN49_RS18835 3507418..3508116 - 699 WP_016530143.1 RluA family pseudouridine synthase
BN49_RS18840 3508307..3508789 + 483 WP_002910657.1 cold shock domain-containing protein
BN49_RS18845 3508899..3509798 + 900 WP_004152315.1 LysR family transcriptional regulator
BN49_RS18850 3509773..3510582 - 810 WP_004899032.1 substrate-binding domain-containing protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01826 experimental BN49_RS18800 (VgrG4) Tse IMU00349 experimental Klebsiella pneumoniae strain Kp52.145 chromosome (NZ_FO834906) 3498991..3501690 (-) 802146982

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
IMU00349 experimental BN49_RS18785 (Sel1E) EFF01826 experimental Klebsiella pneumoniae strain Kp52.145 chromosome (NZ_FO834906) 3496153..3497427 (-) 802146974

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector