Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
T6SS ID | T6SS01216 ![]() |
T6SS type | Type Unknown |
Strain | Klebsiella pneumoniae strain Kp52.145 |
Replicon (RefSeq) | chromosome (NZ_FO834906) |
Location | 3477664..3505840 |
Function | anti-bacterial; anti-eukaryotic |
Comment | Kp52145 T6SS-dependent intra and inter species bacterial competition is dependent on VgrG4 and GST-VgrG4 caused a strong inhibition of yeast growth show VgrG4 is a bona fide T6SS toxin and that the C-terminal extension containing the DUF2345 domain is sufficient for the anti-bacterial and anti-eukaryotic effect |
References | [1] Storey D, McNally A, strand M, Sa-Pessoa Graca Santos J, Rodriguez-Escudero I, Elmore B, Palacios L, Marshall H, Hobley L, Molina M, Cid VJ, Salminen TA, Bengoechea JA. et al (2020) Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent.. PLoS Pathog. 16(3):e1007969. [PudMed:32191774] |
![experimental experimental](images/experimental.png)
T6SS components and genome coordinates
Component | TssA | TssE | TssF | TssG | TssI | TssJ | TssK | TssL |
Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
![](tmp/gene_str_plot/T6SS01216.png)
Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
---|---|---|---|---|---|---|---|
BN49_RS18685 | 3472745..3473791 | - | 1047 | WP_004175547.1 | MerR family transcriptional regulator | ||
BN49_RS18690 (ychH) | 3474012..3474287 | - | 276 | WP_002910437.1 | stress-induced protein YchH | ||
BN49_RS18695 (pth) | 3474560..3475144 | + | 585 | WP_004180389.1 | aminoacyl-tRNA hydrolase | ||
BN49_RS18700 (ychF) | 3475262..3476353 | + | 1092 | WP_002910443.1 | redox-regulated ATPase YchF | ||
BN49_RS18705 | 3476436..3476765 | - | 330 | WP_016530916.1 | DUF1869 domain-containing protein | ||
BN49_RS18710 | 3476850..3477532 | - | 683 | pseudogene | substrate-binding domain-containing protein | ||
T6CP015378 | TssA | BN49_RS18715 | 3477664..3479079 | - | 1416 | WP_046043538.1 | type VI secretion system ImpA family N-terminal domain-containing protein |
T6CP015379 | TssE | BN49_RS18720 (tssE) | 3479099..3479542 | - | 444 | WP_004175538.1 | type VI secretion system baseplate subunit TssE |
T6CP015380 | TssJ | BN49_RS18725 (tssJ) | 3479545..3480081 | - | 537 | WP_077255016.1 | type VI secretion system lipoprotein TssJ |
T6CP015381 | TssG | BN49_RS18730 (tssG) | 3480062..3481078 | - | 1017 | WP_164995970.1 | type VI secretion system baseplate subunit TssG |
T6CP015382 | TssF | BN49_RS18735 (tssF) | 3481108..3482871 | - | 1764 | WP_046043540.1 | type VI secretion system baseplate subunit TssF |
BN49_RS18740 | 3483005..3486395 | - | 3391 | pseudogene | type VI secretion protein VasK | ||
BN49_RS18745 | 3486501..3487646 | - | 1146 | WP_016532067.1 | hypothetical protein | ||
BN49_RS18750 | 3487766..3488029 | - | 264 | WP_016532068.1 | PAAR domain-containing protein | ||
BN49_RS18755 | 3488119..3489096 | - | 978 | WP_016532069.1 | hypothetical protein | ||
BN49_RS18760 | 3489311..3490366 | - | 1056 | WP_129015098.1 | sel1 repeat family protein | ||
BN49_RS18765 | 3490413..3491693 | - | 1281 | WP_046043544.1 | sel1 repeat family protein | ||
BN49_RS18770 | 3491731..3493002 | - | 1272 | WP_038431327.1 | sel1 repeat family protein | ||
BN49_RS18775 | 3493002..3494873 | - | 1872 | WP_046043546.1 | phospholipase | ||
BN49_RS18780 | 3495042..3496115 | - | 1074 | WP_040148336.1 | sel1 repeat family protein | ||
BN49_RS18785 | 3496153..3497427 | - | 1275 | WP_046043550.1 | sel1 repeat family protein | ||
BN49_RS30500 | 3497427..3498994 | - | 1568 | pseudogene | phosphatidylserine/phosphatidylglycerophosphate/ cardiolipin synthase family protein | ||
T6CP015383 | TssI | BN49_RS18800 (vgrG) | 3498991..3501690 | - | 2700 | WP_046043552.1 | type VI secretion system tip protein VgrG |
BN49_RS31145 | 3501841..3501965 | - | 125 | pseudogene | hypothetical protein | ||
BN49_RS18805 | 3502149..3503846 | - | 1698 | WP_032102707.1 | OmpA family protein | ||
T6CP015384 | TssL | BN49_RS18810 | 3503850..3504503 | - | 654 | WP_004891113.1 | type VI secretion system protein TssL, short form |
T6CP015385 | TssK | BN49_RS18815 (tssK) | 3504500..3505840 | - | 1341 | WP_032102706.1 | type VI secretion system baseplate subunit TssK |
BN49_RS18825 | 3506409..3506738 | + | 330 | WP_002910650.1 | gamma-glutamylcyclotransferase | ||
BN49_RS18830 | 3506853..3507392 | + | 540 | WP_002910652.1 | DUF2058 domain-containing protein | ||
BN49_RS18835 | 3507418..3508116 | - | 699 | WP_016530143.1 | RluA family pseudouridine synthase | ||
BN49_RS18840 | 3508307..3508789 | + | 483 | WP_002910657.1 | cold shock domain-containing protein | ||
BN49_RS18845 | 3508899..3509798 | + | 900 | WP_004152315.1 | LysR family transcriptional regulator | ||
BN49_RS18850 | 3509773..3510582 | - | 810 | WP_004899032.1 | substrate-binding domain-containing protein |
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
![experimental experimental](images/experimental.png)
![insilico insolico](images/in_silico.png)
Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
---|
![experimental experimental](images/experimental.png)
![insilico insolico](images/in_silico.png)
Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
---|---|---|---|---|---|---|---|
EFF01826 ![]() |
BN49_RS18800 (VgrG4) | Tse | IMU00349 ![]() |
Klebsiella pneumoniae strain Kp52.145 | chromosome (NZ_FO834906) | 3498991..3501690 (-) | 802146982 |
![experimental experimental](images/experimental.png)
![insilico insolico](images/in_silico.png)
Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
---|---|---|---|---|---|---|
IMU00349 ![]() |
BN49_RS18785 (Sel1E) | EFF01826 ![]() |
Klebsiella pneumoniae strain Kp52.145 | chromosome (NZ_FO834906) | 3496153..3497427 (-) | 802146974 |
![experimental experimental](images/experimental.png)
Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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