Detailed information of component protein


Summary

Component ID T6CP015378
Component protein type TssA
T6SS ID (Type) T6SS01216 experimental (Type Unknown)
Strain Klebsiella pneumoniae strain Kp52.145
Replicon chromosome
Sequence Protein sequence (472 a.a.); Nucleotide sequence (1416 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BN49_RS18715
Coordinate (Strand) 3477664..3479079 (-)
NCBI ID WP_046043538.1
RefSeq NZ_FO834906
Uniprot ID W9BPL4_KLEPN
KEGG ID kpnk:BN49_3355
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VasL PF12486.10 3.2e-52 303..447
ImpA_N PF06812.14 8.3e-21 8..106
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-246 MMTTHHDRHYKAGGDPRTLADFMALRAEMNKLSHPARPDINWPYAEQLARGLLEHHGADLQTVAWYTLARARLGGVAGINEGLTLMESLLVRQGKNLWPQALPAQTEIFRTLSKRLRQVIRTLNLTPEDVESLEQAERSLQSFDAVLQRLEIAPENQLSDLRALLHSTATRFESLDPAPALPTAPPVAVSDAELPGTLVSEEDAAKVEPVPDLKRRPKAEPLAPPSPAKRPAPVAASTPAPVPRWK
Transmembrane helix 247-269 PFIAGMVTMLAVTGIAVGGWLAL
Outside 270-471 RQSDLPPISVTQNAGPIPGLPATTPPGPVDLPQTQRQLGELARLAPDWAVSYGDQLVHHALIRWPDQAQPLAQQWRQQLSAGALPAENITGWSEGMQQLQRLADQLNALDEQKGKYLTVSELKTAVFAITQSFNRAVPLEEQLRQLAALPVDQPWPAARGSLAELHLQQLIVEYALLKRKQPASPTAALPATGEPSVSEAVK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9867 - -


  Protein sequence: 472 a.a.    

>T6CP015378 NZ_FO834906:c3479079-3477664 [Klebsiella pneumoniae] [TssA]
MMTTHHDRHYKAGGDPRTLADFMALRAEMNKLSHPARPDINWPYAEQLARGLLEHHGADLQTVAWYTLARARLGGVAGIN
EGLTLMESLLVRQGKNLWPQALPAQTEIFRTLSKRLRQVIRTLNLTPEDVESLEQAERSLQSFDAVLQRLEIAPENQLSD
LRALLHSTATRFESLDPAPALPTAPPVAVSDAELPGTLVSEEDAAKVEPVPDLKRRPKAEPLAPPSPAKRPAPVAASTPA
PVPRWKPFIAGMVTMLAVTGIAVGGWLALRQSDLPPISVTQNAGPIPGLPATTPPGPVDLPQTQRQLGELARLAPDWAVS
YGDQLVHHALIRWPDQAQPLAQQWRQQLSAGALPAENITGWSEGMQQLQRLADQLNALDEQKGKYLTVSELKTAVFAITQ
SFNRAVPLEEQLRQLAALPVDQPWPAARGSLAELHLQQLIVEYALLKRKQPASPTAALPATGEPSVSEAVK*

  Nucleotide sequence: 1416 bp    

>T6CP015378 NZ_FO834906:c3479079-3477664 [Klebsiella pneumoniae] [TssA]
ATGATGACAACACACCACGACCGTCACTATAAAGCCGGTGGCGACCCGCGCACGCTGGCTGATTTTATGGCCCTGCGTGC
GGAAATGAACAAGCTCAGCCACCCGGCGCGGCCGGATATTAACTGGCCGTATGCCGAGCAGCTTGCCCGCGGGCTGTTGG
AGCATCACGGCGCGGACCTGCAGACTGTCGCCTGGTATACCCTGGCCCGCGCCCGGCTGGGCGGGGTGGCAGGGATCAAT
GAGGGGCTGACGCTGATGGAGTCGCTGCTGGTTCGTCAGGGGAAAAACCTGTGGCCACAGGCGCTCCCGGCCCAGACAGA
GATCTTCCGCACCCTGAGCAAGCGGCTGCGGCAGGTGATCCGTACCCTGAATCTTACCCCAGAGGATGTTGAGTCGCTTG
AGCAGGCTGAACGTTCGCTGCAAAGCTTCGACGCAGTGCTCCAGCGCCTTGAGATCGCTCCTGAAAACCAGCTGAGCGAC
CTGCGGGCGCTACTGCACAGCACGGCAACTCGCTTCGAGAGCCTCGACCCTGCGCCAGCGCTTCCCACCGCGCCGCCGGT
GGCGGTGTCTGACGCGGAACTTCCCGGCACTCTGGTCAGCGAAGAGGATGCGGCGAAGGTTGAGCCAGTGCCAGACCTAA
AACGTAGACCAAAGGCTGAGCCGCTGGCTCCGCCTTCACCTGCAAAGCGGCCCGCGCCGGTGGCGGCGTCTACTCCGGCA
CCCGTGCCGCGCTGGAAGCCGTTTATCGCCGGAATGGTTACCATGCTGGCGGTAACGGGTATCGCCGTCGGCGGATGGCT
GGCGCTGCGTCAGTCCGACTTGCCGCCCATTTCGGTCACCCAAAATGCCGGACCGATACCGGGCCTGCCGGCGACAACGC
CCCCGGGGCCGGTTGATCTGCCACAGACGCAGCGCCAGCTTGGCGAGCTTGCCCGCCTGGCGCCGGACTGGGCGGTCAGC
TACGGCGACCAGCTGGTCCACCATGCTCTTATCCGCTGGCCGGATCAGGCGCAACCGCTGGCGCAGCAGTGGCGGCAGCA
GCTGAGTGCTGGCGCATTGCCCGCGGAAAATATTACCGGCTGGTCGGAGGGCATGCAGCAGCTGCAGCGGCTCGCCGACC
AGCTCAATGCCCTCGACGAGCAGAAGGGGAAGTACCTGACGGTGAGCGAACTGAAAACCGCAGTATTTGCCATCACACAG
TCGTTTAACCGCGCTGTTCCGCTGGAGGAGCAGCTGCGCCAGCTGGCTGCGCTTCCCGTTGATCAACCCTGGCCAGCCGC
GCGGGGAAGCCTCGCGGAGCTGCATCTGCAACAGCTGATAGTGGAGTATGCCCTGCTGAAGCGAAAACAGCCGGCGTCGC
CGACCGCTGCGCTCCCCGCAACCGGTGAGCCCTCGGTGAGTGAGGCGGTGAAATAA