SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015384
Component protein type	TssL
T6SS ID (Type)	T6SS01216 (Type Unknown)
Strain	Klebsiella pneumoniae strain Kp52.145
Replicon	chromosome
Sequence	Protein sequence (218 a.a.); Nucleotide sequence (654 bp)
Reference	[1] Storey D, McNally A, Åstrand M, et al. Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent. PLoS Pathog. 2020 Mar 19;16(3):e1007969. doi: 10.1371/journal.ppat.1007969. eCollection 2020 Mar. PMID: 32191774

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BN49_RS18810
Coordinate (Strand)	3503850..3504503 (-)
NCBI ID	WP_004891113.1
RefSeq	NZ_FO834906
Uniprot ID	A0A652PZ08_9ENTR, W9BPL8_KLEPN, A0A3F3B3W0_KLEPN
KEGG ID	kpnk:BN49_3375
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	1.9e-36	12..202

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-184	MNTSESDLINKTFYPGWLMVSQLRCGQPVTDGEALYRQACRWVTEAREALTAGGVSEASAEQMLYAYCALLDESVLNRASQDDGYRRWRKDPLQARFFSTLNAGEELWERIRQLLREPTADAAVLTCFFRTLQLGFVGQYRAEDDERREDVAQALGARVPPFSLTQEAPVVVRASRLRSGRRMY
Transmembrane helix	185-207	WCGWAAGIVALAALWLTCSAMLS
Outside	208-217	QMVAQIAGQG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9588	-	-

Protein sequence: 218 a.a.

						>T6CP015384 NZ_FO834906:c3504503-3503850 [Klebsiella pneumoniae] [TssL]
MNTSESDLINKTFYPGWLMVSQLRCGQPVTDGEALYRQACRWVTEAREALTAGGVSEASAEQMLYAYCALLDESVLNRAS
QDDGYRRWRKDPLQARFFSTLNAGEELWERIRQLLREPTADAAVLTCFFRTLQLGFVGQYRAEDDERREDVAQALGARVP
PFSLTQEAPVVVRASRLRSGRRMYWCGWAAGIVALAALWLTCSAMLSQMVAQIAGQG*					

Nucleotide sequence: 654 bp

						>T6CP015384 NZ_FO834906:c3504503-3503850 [Klebsiella pneumoniae] [TssL]
ATGAATACCTCTGAAAGCGATCTGATCAACAAAACTTTTTACCCGGGCTGGCTGATGGTCAGCCAGTTACGCTGCGGCCA
GCCGGTGACAGATGGCGAGGCGCTCTATCGCCAGGCCTGTCGATGGGTGACCGAGGCGCGCGAGGCGCTGACGGCGGGGG
GAGTCAGTGAAGCCAGCGCTGAGCAGATGCTCTATGCCTACTGCGCGCTGCTCGATGAGAGCGTCCTCAACCGCGCCAGT
CAGGATGATGGCTACCGCAGGTGGCGCAAGGATCCGCTGCAGGCGCGCTTTTTCAGCACTCTCAACGCCGGGGAAGAGCT
CTGGGAACGGATCCGCCAGCTGCTGCGAGAGCCCACGGCGGATGCGGCAGTGCTGACCTGCTTCTTTCGTACTTTACAGC
TGGGATTTGTCGGACAGTACCGCGCCGAGGATGACGAACGGCGTGAGGACGTGGCGCAAGCGCTTGGCGCGCGGGTCCCG
CCGTTCAGCCTGACCCAGGAGGCGCCGGTGGTGGTTCGTGCCTCCCGGCTGCGCAGCGGACGGCGGATGTACTGGTGCGG
CTGGGCGGCCGGCATTGTGGCGCTGGCGGCGCTGTGGTTGACCTGCTCCGCCATGCTGTCACAGATGGTGGCGCAGATAG
CAGGGCAGGGATAA					