SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015381
Component protein type	TssG
T6SS ID (Type)	T6SS01216 (Type Unknown)
Strain	Klebsiella pneumoniae strain Kp52.145
Replicon	chromosome
Sequence	Protein sequence (339 a.a.); Nucleotide sequence (1017 bp)
Reference	[1] Storey D, McNally A, Åstrand M, et al. Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent. PLoS Pathog. 2020 Mar 19;16(3):e1007969. doi: 10.1371/journal.ppat.1007969. eCollection 2020 Mar. PMID: 32191774

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BN49_RS18730
Coordinate (Strand)	3480062..3481078 (-)
NCBI ID	WP_164995970.1
RefSeq	NZ_FO834906
Uniprot ID	UPI0013ECE175
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.5e-73	1..298

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-338	MNFYRFCQLLEKNHPKAPIIGSGWLVGDEPIRFRPHPGMGFPAGEIRGMDDPEPPRPPAVHVTFMGLYGVESPLPTHYSDDIAQRREGVEATEDFLDIFNHRLIAQYYRIWRKYSYPATFRAGGTDNISQYLLGLAGLGIPGCAAVAASPLSRFLALLPVMMLPGRSGEGMEALVALLAPGTRATVYHHDPCRIPLSQPLTMSVRQPVSLQHRPVMGTHATDVNGQVLLQLATEKPDEVRGWLPGGELFSDLMALLHVWLGSHLDVRLQLCVARHLLPDAQLCCQQEHAVQLGRTAVLRPLDAQKQADDRVTIYLGRYQRVRENIHRRESDEDGDYRS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9776	-	-

Protein sequence: 339 a.a.

						>T6CP015381 NZ_FO834906:c3481078-3480062 [Klebsiella pneumoniae] [TssG]
MNFYRFCQLLEKNHPKAPIIGSGWLVGDEPIRFRPHPGMGFPAGEIRGMDDPEPPRPPAVHVTFMGLYGVESPLPTHYSD
DIAQRREGVEATEDFLDIFNHRLIAQYYRIWRKYSYPATFRAGGTDNISQYLLGLAGLGIPGCAAVAASPLSRFLALLPV
MMLPGRSGEGMEALVALLAPGTRATVYHHDPCRIPLSQPLTMSVRQPVSLQHRPVMGTHATDVNGQVLLQLATEKPDEVR
GWLPGGELFSDLMALLHVWLGSHLDVRLQLCVARHLLPDAQLCCQQEHAVQLGRTAVLRPLDAQKQADDRVTIYLGRYQR
VRENIHRRESDEDGDYRS*					

Nucleotide sequence: 1017 bp

						>T6CP015381 NZ_FO834906:c3481078-3480062 [Klebsiella pneumoniae] [TssG]
ATGAATTTTTACCGCTTCTGCCAGTTGCTGGAGAAGAACCATCCGAAGGCGCCGATCATCGGCAGCGGCTGGCTAGTAGG
TGATGAACCCATACGCTTTCGCCCGCATCCGGGGATGGGCTTTCCGGCTGGCGAAATCCGCGGAATGGACGATCCTGAAC
CGCCCCGTCCGCCGGCGGTTCACGTGACTTTTATGGGACTCTACGGCGTGGAATCCCCGCTGCCGACCCACTATAGCGAT
GACATTGCGCAACGGCGGGAAGGCGTCGAGGCCACCGAGGATTTCCTCGATATCTTTAACCATCGGCTGATCGCCCAGTA
TTACCGTATATGGCGCAAATATTCCTACCCGGCAACCTTTCGGGCGGGCGGCACGGACAACATCTCGCAGTATCTGCTGG
GCCTGGCGGGGCTGGGCATACCGGGATGTGCCGCCGTTGCCGCATCGCCGCTGTCGCGATTTCTGGCGCTGCTGCCGGTG
ATGATGCTGCCGGGGAGATCCGGGGAAGGCATGGAGGCGCTGGTGGCGCTCCTGGCCCCGGGGACCCGGGCGACGGTGTA
CCACCACGATCCCTGCCGGATCCCCTTGTCGCAGCCGTTAACGATGAGCGTCCGTCAGCCGGTTAGCCTGCAACATAGGC
CAGTGATGGGAACCCACGCCACGGATGTGAACGGTCAGGTATTGCTGCAGCTGGCCACGGAAAAGCCTGATGAGGTTCGC
GGCTGGCTGCCCGGCGGCGAACTGTTCAGCGACCTGATGGCGCTTCTGCACGTCTGGCTCGGATCTCATCTTGACGTGCG
GCTGCAGCTTTGCGTCGCCCGCCATTTACTGCCGGATGCGCAACTTTGCTGCCAGCAGGAGCATGCCGTCCAGCTCGGCC
GTACCGCTGTGCTGCGTCCTCTGGATGCGCAGAAACAGGCTGACGACAGAGTCACGATTTATCTGGGGCGCTATCAGCGC
GTCCGGGAAAATATCCACCGCAGGGAGAGCGATGAAGATGGCGATTACCGCAGTTAA					