Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00329  |
| Cognate effector | EFF01525 |
| Related T6SS (Type) | T6SS00946 (Type Unknown) |
| Strain | Escherichia coli strain PE117 |
| Replicon | chromosome |
| Sequence | Protein sequence (184 a.a.); Nucleotide sequence (552 bp) |
| Description | The complementation of vti5 in Δvt5Δvti5 provided effective protection against growth inhibition by vti1+ Wt-PE171 strains |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | vt4C (VTI5) |
| Coordinates (Strand) | 3140..3691 (+) |
| NCBI ID | 1062685230 |
| GenBank | KX118293 |
| Uniprot ID | A0A1Y0R511_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-183 | MRIMLILLCFFSFPCFSGDKIKIVKTCQLKKNESVQLLSLRTIDGDTLYLKFRNVITGAFLDTPTKSYDFFGDVVLSQCINGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPEAVWFGKNNILIIIRNQMDVGTWKGKYIIYDNAKKSGERAYSSDNLPSKDGYIIFALNK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.6332 | pos: 14-15. SFP-CF. | MRIMLILLCFFSFP |
Protein sequence: 184 a.a. .
>IMU00329 KX118293:3140-3691 [Escherichia coli]
MRIMLILLCFFSFPCFSGDKIKIVKTCQLKKNESVQLLSLRTIDGDTLYLKFRNVITGAFLDTPTKSYDFFGDVVLSQCI
NGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPEAVWFGKNNILIIIRNQMDVGTWKGKYIIYDNAKKS
GERAYSSDNLPSKDGYIIFALNK*
MRIMLILLCFFSFPCFSGDKIKIVKTCQLKKNESVQLLSLRTIDGDTLYLKFRNVITGAFLDTPTKSYDFFGDVVLSQCI
NGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPEAVWFGKNNILIIIRNQMDVGTWKGKYIIYDNAKKS
GERAYSSDNLPSKDGYIIFALNK*
Nucleotide sequence: 552 bp .
>IMU00329 KX118293:3140-3691 [Escherichia coli]
ATGCGCATTATGTTAATATTACTTTGTTTTTTTTCTTTCCCCTGTTTTTCAGGCGATAAGATTAAAATCGTGAAAACCTG
TCAACTGAAAAAAAATGAAAGTGTGCAGTTGCTTTCCCTTAGAACCATTGACGGTGATACATTATATCTAAAATTCAGAA
ATGTTATCACCGGGGCCTTTTTAGATACTCCAACTAAAAGTTATGATTTTTTTGGAGATGTGGTTCTTTCTCAGTGCATA
AATGGTTCTCTTATCTTTGCGCTGGAATATGGTTCTCCATATATAAAAGGTTGTCTGGTAACTGGTTGGGAAAATGGGTT
TAAAGGTAAGAACTCTCCTGAAGGCTTTTGTTTCGCAGAGAGAAATATTCCCGAAGCGGTATGGTTTGGAAAAAACAATA
TTTTAATTATCATCCGAAACCAAATGGATGTTGGGACATGGAAAGGGAAATATATTATCTATGATAATGCGAAAAAGTCA
GGAGAAAGAGCATACTCATCAGATAATCTACCATCAAAAGATGGTTACATAATTTTTGCATTAAATAAATAA
ATGCGCATTATGTTAATATTACTTTGTTTTTTTTCTTTCCCCTGTTTTTCAGGCGATAAGATTAAAATCGTGAAAACCTG
TCAACTGAAAAAAAATGAAAGTGTGCAGTTGCTTTCCCTTAGAACCATTGACGGTGATACATTATATCTAAAATTCAGAA
ATGTTATCACCGGGGCCTTTTTAGATACTCCAACTAAAAGTTATGATTTTTTTGGAGATGTGGTTCTTTCTCAGTGCATA
AATGGTTCTCTTATCTTTGCGCTGGAATATGGTTCTCCATATATAAAAGGTTGTCTGGTAACTGGTTGGGAAAATGGGTT
TAAAGGTAAGAACTCTCCTGAAGGCTTTTGTTTCGCAGAGAGAAATATTCCCGAAGCGGTATGGTTTGGAAAAAACAATA
TTTTAATTATCATCCGAAACCAAATGGATGTTGGGACATGGAAAGGGAAATATATTATCTATGATAATGCGAAAAAGTCA
GGAGAAAGAGCATACTCATCAGATAATCTACCATCAAAAGATGGTTACATAATTTTTGCATTAAATAAATAA