Detailed information of immunity protein


Summary

Immunity protein ID IMU00329 experimental
Cognate effector EFF01525 experimental
Related T6SS (Type) T6SS00946 experimental (Type Unknown)
Strain Escherichia coli strain PE117
Replicon chromosome
Sequence Protein sequence (184 a.a.); Nucleotide sequence (552 bp)
Description The complementation of vti5 in Δvt5Δvti5 provided effective protection against growth inhibition by vti1+ Wt-PE171 strains
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) vt4C (VTI5)
Coordinates (Strand) 3140..3691 (+)
NCBI ID 1062685230
GenBank KX118293
Uniprot ID A0A1Y0R511_ECOLX
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-183 MRIMLILLCFFSFPCFSGDKIKIVKTCQLKKNESVQLLSLRTIDGDTLYLKFRNVITGAFLDTPTKSYDFFGDVVLSQCINGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPEAVWFGKNNILIIIRNQMDVGTWKGKYIIYDNAKKSGERAYSSDNLPSKDGYIIFALNK



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.6332 pos: 14-15. SFP-CF. MRIMLILLCFFSFP



  Protein sequence: 184 a.a.    .

>IMU00329 KX118293:3140-3691 [Escherichia coli]
MRIMLILLCFFSFPCFSGDKIKIVKTCQLKKNESVQLLSLRTIDGDTLYLKFRNVITGAFLDTPTKSYDFFGDVVLSQCI
NGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPEAVWFGKNNILIIIRNQMDVGTWKGKYIIYDNAKKS
GERAYSSDNLPSKDGYIIFALNK*

  Nucleotide sequence: 552 bp    .

>IMU00329 KX118293:3140-3691 [Escherichia coli]
ATGCGCATTATGTTAATATTACTTTGTTTTTTTTCTTTCCCCTGTTTTTCAGGCGATAAGATTAAAATCGTGAAAACCTG
TCAACTGAAAAAAAATGAAAGTGTGCAGTTGCTTTCCCTTAGAACCATTGACGGTGATACATTATATCTAAAATTCAGAA
ATGTTATCACCGGGGCCTTTTTAGATACTCCAACTAAAAGTTATGATTTTTTTGGAGATGTGGTTCTTTCTCAGTGCATA
AATGGTTCTCTTATCTTTGCGCTGGAATATGGTTCTCCATATATAAAAGGTTGTCTGGTAACTGGTTGGGAAAATGGGTT
TAAAGGTAAGAACTCTCCTGAAGGCTTTTGTTTCGCAGAGAGAAATATTCCCGAAGCGGTATGGTTTGGAAAAAACAATA
TTTTAATTATCATCCGAAACCAAATGGATGTTGGGACATGGAAAGGGAAATATATTATCTATGATAATGCGAAAAAGTCA
GGAGAAAGAGCATACTCATCAGATAATCTACCATCAAAAGATGGTTACATAATTTTTGCATTAAATAAATAA