Detailed information of effector
Summary
| Effector ID | EFF01525  |
| Effector type | Tge |
| Cognate immunity protein | IMU00329 |
| Related T6SS (Type) | T6SS00946 (Type Unknown) |
| Strain | Escherichia coli strain PE117 |
| Replicon | chromosome |
| Sequence | Protein sequence (201 a.a.); Nucleotide sequence (603 bp) |
| Description | VT5 is a T6SS lysozyme-like, a cell-wall targeting T6SS effector of the peptidoglycan glycoside hydrolase family |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | vt4B (VT5) |
| Coordinates (Strand) | 2536..3138 (+) |
| NCBI ID | 1062685230 |
| GenBank | KX118293 |
| Uniprot ID | A0A1Y0R511_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| SLT | PF01464.22 | 8.4e-06 | 68..168 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-200 | MADKSEKKKETTNKSAKKTLTKKSEKLTPAKDTAPKQVNVDPAIRLKQIRQLVRNNNKSIMGEDYIICQIYKESRFKQFAGKNKHNAKGLMQMQRNAVRQVFKYRQQKIKGRMTTDKETNEAFANADTFYKSDKIFDEKENIKIGTEYLQYWIDKEATIEEAYRAYRGTDEAYYSVIKPCAEKLAKDPDNIQILMEGIGR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9836 | - | - |
Protein sequence: 201 a.a. .
>EFF01525 KX118293:2536-3138 Type VI Glycoside hydrolase Effector (Tge) [Escherichia coli]
MADKSEKKKETTNKSAKKTLTKKSEKLTPAKDTAPKQVNVDPAIRLKQIRQLVRNNNKSIMGEDYIICQIYKESRFKQFA
GKNKHNAKGLMQMQRNAVRQVFKYRQQKIKGRMTTDKETNEAFANADTFYKSDKIFDEKENIKIGTEYLQYWIDKEATIE
EAYRAYRGTDEAYYSVIKPCAEKLAKDPDNIQILMEGIGR*
MADKSEKKKETTNKSAKKTLTKKSEKLTPAKDTAPKQVNVDPAIRLKQIRQLVRNNNKSIMGEDYIICQIYKESRFKQFA
GKNKHNAKGLMQMQRNAVRQVFKYRQQKIKGRMTTDKETNEAFANADTFYKSDKIFDEKENIKIGTEYLQYWIDKEATIE
EAYRAYRGTDEAYYSVIKPCAEKLAKDPDNIQILMEGIGR*
Nucleotide sequence: 603 bp .
>EFF01525 KX118293:2536-3138 Type VI Glycoside hydrolase Effector (Tge) [Escherichia coli]
ATGGCAGATAAATCCGAAAAGAAAAAAGAAACCACAAATAAATCAGCCAAAAAAACACTAACGAAGAAGAGCGAAAAATT
AACTCCAGCAAAAGACACCGCACCAAAACAAGTGAATGTAGATCCAGCTATAAGATTGAAACAAATTCGTCAACTTGTCC
GTAATAATAATAAATCCATCATGGGAGAGGATTATATTATTTGTCAAATCTATAAAGAATCTAGATTTAAGCAATTTGCA
GGTAAGAATAAACATAATGCGAAAGGCTTAATGCAAATGCAACGAAATGCAGTGAGGCAGGTTTTTAAATATCGACAACA
AAAAATAAAAGGAAGAATGACGACTGATAAAGAGACAAATGAAGCTTTCGCTAATGCTGATACATTTTATAAATCTGATA
AAATATTTGATGAAAAAGAAAATATTAAGATTGGCACAGAGTATTTGCAATACTGGATAGATAAAGAAGCAACAATTGAG
GAGGCATATCGGGCATATCGTGGAACAGATGAAGCATACTATTCTGTTATAAAACCTTGCGCTGAAAAACTGGCTAAAGA
TCCTGATAACATACAAATACTAATGGAGGGAATAGGAAGATAG
ATGGCAGATAAATCCGAAAAGAAAAAAGAAACCACAAATAAATCAGCCAAAAAAACACTAACGAAGAAGAGCGAAAAATT
AACTCCAGCAAAAGACACCGCACCAAAACAAGTGAATGTAGATCCAGCTATAAGATTGAAACAAATTCGTCAACTTGTCC
GTAATAATAATAAATCCATCATGGGAGAGGATTATATTATTTGTCAAATCTATAAAGAATCTAGATTTAAGCAATTTGCA
GGTAAGAATAAACATAATGCGAAAGGCTTAATGCAAATGCAACGAAATGCAGTGAGGCAGGTTTTTAAATATCGACAACA
AAAAATAAAAGGAAGAATGACGACTGATAAAGAGACAAATGAAGCTTTCGCTAATGCTGATACATTTTATAAATCTGATA
AAATATTTGATGAAAAAGAAAATATTAAGATTGGCACAGAGTATTTGCAATACTGGATAGATAAAGAAGCAACAATTGAG
GAGGCATATCGGGCATATCGTGGAACAGATGAAGCATACTATTCTGTTATAAAACCTTGCGCTGAAAAACTGGCTAAAGA
TCCTGATAACATACAAATACTAATGGAGGGAATAGGAAGATAG