SecReT6, T6SS database

Detailed information of Recipient susceptibility factor

Summary

ID	RSF000014
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (230 a.a.); Nucleotide sequence (690 bp)
Response to T6SS attack	sensitive
Known or putative function	phosphate response regulator transcription factor
Description	Survival (killing by TseHWT/TseHH64a ) of E. coli ΔdegP mutant with vector (Vec) or overexpressing genes from yeeN and phoB is significant different.
Reference	[1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

External database links

Locus tag (Gene)	BW25113_RS02065 (phoB)
Coordinates (Strand)	412598..413287 (+)
NCBI ID	446036078
RefSeq	NZ_CP009273
Uniprot ID	A0A1Z3UU77_ECOLX, M9FZT3_ECOLX, A0A0E0Y4K3_ECO1C, K4WUV2_ECOLX, S1Q746_ECOLX, L3BU95_ECOLX, S1EVX8_ECOLX, L3QFZ3_ECOLX, S1I5B4_ECOLX, L4JFF0_ECOLX, U9XX77_ECOLX, U9XU55_ECOLX, V0SXG8_ECOLX, U9Z5Z5_ECOLX, V0URF9_ECOLX, V0XND1_ECOLX, V0ZCN5_ECOLX, V0AGY7_ECOLX, A0A0E2LNM3_ECOU3, W0ARK6_9ESCH, A0A080JA26_ECOLX, A0A029IB42_ECOLX, A0A2T3RXC0_9ESCH, D3GU91_ECO44, E3PFL5_ECOH1, A7ZIE9_ECO24, A0A0E1SZ49_ECOLX, Q1RFE3_ECOUT, A0A0H2YWN4_ECOK1, B6HZJ8_ECOSE, B1LIT3_ECOSM, A0A140NB44_ECOBD, J7QXZ0_ECOLX, A0A0H3PVM7_ECO5C, B1EKU1_ESCAT, D6I7B0_ECOLX, D6J6X4_ECOLX, A0A1X3JT03_9ESCH, F4NMF1_ECOLX, E0J0P0_ECOLW, A0A169RV99_KLEOX, B7UJM2_ECO27, B7MD57_ECO45, B7L553_ECO55, B7N8V0_ECOLU, A0A0H3MED7_ECO7I, B7MPF9_ECO81, A0A1Q8MKR3_SHIBO, A0A0I0SFM1_SHISO, A0A1X3JM12_ECOLX, A0A1X3L7D8_ECOLX, A0A1X3K875_ECOLX, F4VAH8_ECOLX, A0A1X3KPU9_ECOLX, F4SJ11_ECOLX, A0A1X3IC76_ECOLX, A0A0K9TB40_ECOLX, F4SUL3_ECOLX, F4TAQ4_ECOLX, A0A1X3LNC6_ECOLX, A0A1X3LTP1_ECOLX, A0A1X3J5Y3_ECOLX, E9XPQ6_ECOLX, E6BHM7_ECOLX, E1IPJ9_ECOLX, E1IZS4_ECOLX, D8E161_ECOLX, A0A0H3EGH5_ECO8N, D3QJG6_ECOCB, A0A0E1LYE4_ECOLX, D8A4G4_ECOMS, D7XKD3_ECOLX, D7Y372_ECOM1, E9TLI8_ECOLX, I4SYP4_ECOLX, PHOB_ECOLI, PHOB_ECO57, I2SZW0_ECOLX, I2UR06_ECOLX, I2WQL0_ECOLX, A0A192CIK5_ECOLX, W8ZF66_ECOLX
KEGG ID	ecz:ECS88_0393, ecy:ECSE_0419, elh:ETEC_0451, eln:NRG857_01870, elw:ECW_m0468, ece:Z0497, ecq:ECED1_0421, ecos:EC958_0537, eum:ECUMN_0436, ecj:JW0389, ecw:EcE24377A_0427, eci:UTI89_C0420, eco:b0399, elo:EC042_0431, ecv:APECO1_1611, eck:EC55989_0407, ecm:EcSMS35_0429, eok:G2583_0507, ebd:ECBD_3262, ecs:ECs0449, esl:O3K_19520, ecg:E2348C_0334, ect:ECIAI39_0283, eal:EAKF1_ch1041c
PDB ID	1QQI, 1GXP, 1GXQ, 2JB9, 1ZES, 2JBA, 2IYN, 3T72, 1B00, 2Z33

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Response_reg	PF00072.26	5.2e-29	5..117
Trans_reg_C	PF00486.30	1.2e-28	151..225

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-229	MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEFKLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9949	-	-

Protein sequence: 230 a.a. .

                                    >RSF000014 NZ_CP009273:412598-413287 [Escherichia coli BW25113]
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVV
MLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF*

                                

Nucleotide sequence: 690 bp .

                                    >RSF000014 NZ_CP009273:412598-413287 [Escherichia coli BW25113]
ATGGCGAGACGTATTCTGGTCGTAGAAGATGAAGCTCCAATTCGCGAAATGGTCTGCTTCGTGCTCGAACAAAATGGCTT
TCAGCCGGTCGAAGCGGAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTCGACT
GGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTG
ATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCGTT
TTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGA
TGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTT
AAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAA
CGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCA
TGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAA

                                