Detailed information of Recipient susceptibility factor


Summary

ID RSF000060 experimental
Strain Klebsiella pneumoniae 30660/NJST258_1
Replicon chromosome
Sequence Protein sequence (378 a.a.); Nucleotide sequence (1134 bp)
Response to T6SS attack protective
Known or putative function capsule protection
Description Group I capsules confer a strong protection from V. cholerae's T6SS attacks to commensal Klebsiella strains.
Reference
experimental Experimental investigation has been performed.
External database links

Locus tag (Gene) KPNJ1_RS09040 (wza)
Coordinates (Strand) 1798389..1799522 (+)
NCBI ID WP_004151152.1
RefSeq NZ_CP006923
Uniprot ID A0A0E1CBR6_KLEPN, W8UFC1_KLEPN, A0A024GWC8_KLEPN, H2CZH6_KLEPN
KEGG ID kpa:KPNJ1_01854, kps:KPNJ2_01819, kpg:KPNIH32_18380
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Poly_export PF02563.18 1.1e-27 82..165
Wza_C PF18412.3 6.3e-17 345..374
SLBB PF10531.11 6.3e-09 173..222
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MKKK
Transmembrane helix 5-27 IVRFSALALAIGFLSGCTIIPGQ
Outside 28-377 GLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPETVLARPNPQLDNLLRSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSQVRQDIASRLTTYIESPQVDVSIAAFRSQKAYVTGEVAKSGQQPITNIPLTVMDAINAAGGLSEDADWRNVVLTHNGKDTKISLYALMQKGDLTENRLLYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGMTLAEALGNAEGMSQAFSDATGVFVIRQLKGDKKGKIANIYQLNAQDASAMVLGTEFQLQPYDIVYVTTAPLVRWNRVISQLVPTITGVHDITETVKYVKDM


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9982 pos: 20-21. LSG-CT. MKKKIVRFSALALAIGFLSG


  Protein sequence: 378 a.a.    .

>RSF000060 NZ_CP006923:1798389-1799522 [Klebsiella pneumoniae 30660/NJST258_1]
MKKKIVRFSALALAIGFLSGCTIIPGQGLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPETVLARPNPQLDNLL
RSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSQVRQDIASRLTTYIESPQ
VDVSIAAFRSQKAYVTGEVAKSGQQPITNIPLTVMDAINAAGGLSEDADWRNVVLTHNGKDTKISLYALMQKGDLTENRL
LYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGMTLAEALGNAEGMSQAFSDATGVFVIRQLKGDKKGKIANIYQLNA
QDASAMVLGTEFQLQPYDIVYVTTAPLVRWNRVISQLVPTITGVHDITETVKYVKDM*

  Nucleotide sequence: 1134 bp    .

>RSF000060 NZ_CP006923:1798389-1799522 [Klebsiella pneumoniae 30660/NJST258_1]
ATGAAGAAAAAAATTGTTAGATTTTCGGCATTAGCGTTGGCAATTGGGTTTTTATCGGGTTGTACCATTATCCCTGGTCA
GGGATTAAACAGTCTGCGCAAGAATGTGGTTGAGCTACCGGACAGCGACTACGATCTGGATAAGCTAGTCAATGTTTACC
CTATGACTCCAGGGCTGATCGATCAGCTTCGTCCAGAGACCGTTCTTGCTCGTCCCAACCCGCAGCTGGATAACTTACTT
CGTAGTTATGAATACCGTATTGGAGTGGGTGATGTGCTGATGGTTACCGTATGGGACCATCCGGAACTCACAACGCCTGC
AGGTCAATACCGTAGCGCCAGTGACACCGGTAACTGGGTGAACTCAGATGGCACCATTTTCTATCCTTATATCGGGAAGG
TGCAGGTGGCGGGAAAAACATTAAGCCAGGTACGCCAGGATATAGCGAGTCGCTTGACTACTTATATTGAAAGCCCTCAG
GTTGACGTGAGTATTGCTGCATTCCGCTCGCAAAAAGCCTATGTGACTGGGGAGGTTGCTAAATCAGGACAGCAACCGAT
TACGAATATTCCGTTGACTGTCATGGATGCAATCAATGCTGCAGGTGGCTTATCAGAAGACGCTGACTGGCGTAATGTGG
TGCTGACGCATAATGGTAAGGACACCAAAATCTCGCTATATGCCCTTATGCAGAAAGGTGATTTGACAGAAAACCGCTTG
CTGTACCCAGGGGATATTCTGTTTGTCCCTCGTAATGATGATCTTAAAGTTTTCGTGATGGGTGAAGTTGGTAAACAAAG
CACGATGAAAATGGATCGTAGTGGTATGACCTTGGCAGAAGCTTTGGGTAATGCAGAAGGCATGTCACAAGCTTTCAGTG
ATGCAACAGGTGTTTTTGTTATCCGTCAGCTCAAGGGTGATAAAAAAGGCAAAATAGCGAATATTTATCAACTTAATGCC
CAAGATGCATCAGCTATGGTATTAGGTACGGAATTCCAGCTTCAGCCTTATGATATCGTGTACGTTACTACAGCTCCGCT
GGTCCGTTGGAATAGAGTAATATCACAATTAGTACCGACAATTACAGGAGTACATGATATTACCGAAACAGTTAAATACG
TTAAGGATATGTGA