Detailed information of Recipient susceptibility factor

Summary

ID	RSF000001
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (217 a.a.); Nucleotide sequence (651 bp)
Response to T6SS attack	protective
Known or putative function	RcsB regulon; response envelope stress
Description	Deletion of the response regulators rcsB or baeR rendered E. coli sensitive to killing by V. cholerae TseH, significantly reducing survival. Complementation by exogenous expression of the deleted genes restored survival. These data indicate that the Rcs and BaeSR ESRs are critical for complete survival of E. coli against T6SS-delivered TseH
Reference	[1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [3] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

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External database links

Locus tag (Gene)	BW25113_RS11580 (RcsB/ymgA)
Coordinates (Strand)	2309656..2310306 (+)
NCBI ID	446984661
RefSeq	NZ_CP009273
Uniprot ID	A0A1Z3V1P7_ECOLX, M9G8H4_ECOLX, A0A0E0Y073_ECO1C, K4Y299_ECOLX, S1PG98_ECOLX, L3C827_ECOLX, S1F473_ECOLX, L3Q2N3_ECOLX, S1HSY7_ECOLX, L4J632_ECOLX, A0A0F6RFA2_CITAM, U9YD36_ECOLX, U9YL55_ECOLX, V0SF26_ECOLX, U9Z989_ECOLX, V0UR83_ECOLX, V0XRY5_ECOLX, V0ZFU1_ECOLX, U9ZZ96_ECOLX, A0A0E2LJT6_ECOU3, A0A080IUV1_ECOLX, A0A0A0FDX6_ECOLX, RCSB_ECOL6, A0A2K9P6I0_9ENTR, D3H0Q7_ECO44, A8ADZ6_CITK8, Q32I20_SHIDS, E3PDR9_ECOH1, A7ZP39_ECO24, A0A2S4S3B2_CITAM, A0A0H2Z1B9_ECOK1, B6I7H9_ECOSE, B1LKW4_ECOSM, J7RSZ7_ECOLX, A0A0H3PXI1_ECO5C, A0A078LB51_CITKO, D6IC33_ECOLX, D6JCB8_ECOLX, A0A0M3F799_ENTCL, RCSB_ECOLX, E0IY85_ECOLW, A0A181WN86_KLEOX, B7UFN1_ECO27, B7MFD0_ECO45, B7LAP1_ECO55, B7N5H9_ECOLU, A0A0H3MS51_ECO7I, B7MXP8_ECO81, A0A1Q8M5C2_SHIBO, A0A1S9K1N2_SHIDY, RCSB_SHIFL, A0A0I2FRE2_SHISO, A0A0K9TA32_ECOLX, A0A1X3LLE3_ECOLX, A0A1X3LZJ9_ECOLX, E9XL29_ECOLX, A0A223JU00_9ENTR, E6BKG3_ECOLX, E1IJN9_ECOLX, E1JDV8_ECOLX, D8E7Q5_ECOLX, A0A0H3EL51_ECO8N, D3QKS0_ECOCB, A0A0E1M3W6_ECOLX, D8A5G4_ECOMS, D7XL34_ECOLX, D7Y4X8_ECOM1, E9T9C7_ECOLX, I4T573_ECOLX, RCSB_ECOLI, RCSB_ECO57, J9JEG3_ECO57, I2SY44_ECOLX, I2UP38_ECOLX, I2WNX7_ECOLX
KEGG ID	elw:ECW_m2419, ecq:ECED1_2683, ect:ECIAI39_2355, cko:CKO_00553, ecv:APECO1_4342, cama:F384_11900, sfl:SF2301, ecg:E2348C_2362, sdy:SDY_0857, ecs:ECs3106, ecy:ECSE_2485, sfx:S2432, eum:ECUMN_2554, eok:G2583_2759, ecw:EcE24377A_2517, ecz:ECS88_2366, ecj:JW2205, eck:EC55989_2472, eco:b2217, ecm:EcSMS35_2366, ecc:c2760, eln:NRG857_11250, cfar:CI104_17235, elh:ETEC_2351, caf:AL524_10995, elo:EC042_2458, esl:O3K_08360, ece:Z3476
PDB ID	5VXN, 5W43, 5I4C

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Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Response_reg	PF00072.26	1.4e-24	6..120
GerE	PF00196.21	3.4e-12	151..201

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Transmembrane helices

• Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-216	MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINNLPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLDIEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLRLFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD

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Signal peptides

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9975	-	-

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  Protein sequence: 217 a.a.    .Download


  Nucleotide sequence: 651 bp    .Download