Detailed information of regulator


Summary

Regulator ID REG0248 experimental
Regulator type protein
Regulatory pathway (from literatures) Transcription regulator
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00464 experimental (Type i4b)
Strain Escherichia coli 042
Replicon plasmid
Sequence Protein sequence (266 a.a.); Nucleotide sequence (798 bp)
Description ; 32 genes encoded within the PAI were expressed at least twofold higher in the wild‐type strain than in 042aggR These genes included aaiA through aaiY and coincides two‐dimensional gel electrophoresis (2‐D PAGE) result; AggR autoactivates its expression, with the DNA sequence extending from bases −50 to +114 relative to the predicted transcriptional start being necessary and sufficient for this autoregulation. EMSA suggested two AggR-binding sites within the −82 to +114 region, DNAse I footprinting was consistent with the EMSA data, two discrete MBP–AggR footprints were observed.
Reference
External database links

Locus tag (Gene) EC042_RS26480 (aggR)
Coordinates (Strand) 41080..41877 (-)
NCBI ID 446692110
RefSeq NC_017627
Uniprot ID D3H542_ECO44
KEGG ID elo:EC042_pAA052
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
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Target
Regulation (↓/↑)
Target sequence
EC042_RS26480 (aggR) promoter↓ATTAACAAACGTATTTTATATGAGTTAAAAATATATCTTTTTATTGATAAGAGTTAGGTCATTCTAACGCAGATTGCCTGATAAAGACATTTTTTTCATGTGAGAATGATATGAAATTAAAACAAAACATCGAAAAAGA
EC042_RS26480 (aggR) promoter↓ATTAACAAACGTATTTTATATGAGTTAAAAATATATCTTTTTATTGATAAGAGTTAGGTCATTCTAACGCAGATTGCCTGATAAAGACATTTTTTTCATGTGAGAATGATATGAAATTAAAACAAAACATCGAAAAAGA
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
HTH_18PF12833.92.4e-13183..259
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-265MKLKQNIEKEIIKINNIRIHQYTVLYTSNCTIDVYTKEGSNTYLRNELIFLERGINISVRLQKKKSTANPFIAIRLSSDTLRRLKDALMIIYGISKVDACSCPNWSKGIIVADADDSVLDTFKSIDNNDDSRITSDLIYLISKIENNKKIIESIYISAVSFFSDKVRNIIEKDLSKRWTLAIIADEFNVSEITIRKRLESEYITFNQILMQSRMSKAALLLLDNSYQISQISNMIGFSSTSYFIRLFVKHFGITPKQFLTYFKSQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
Other0.9449--


  Protein sequence of regulator: 266 a.a.    .

>REG0248 NC_017627:c41877-41080 [Escherichia coli 042]
MKLKQNIEKEIIKINNIRIHQYTVLYTSNCTIDVYTKEGSNTYLRNELIFLERGINISVRLQKKKSTANPFIAIRLSSDT
LRRLKDALMIIYGISKVDACSCPNWSKGIIVADADDSVLDTFKSIDNNDDSRITSDLIYLISKIENNKKIIESIYISAVS
FFSDKVRNIIEKDLSKRWTLAIIADEFNVSEITIRKRLESEYITFNQILMQSRMSKAALLLLDNSYQISQISNMIGFSST
SYFIRLFVKHFGITPKQFLTYFKSQ*

  Nucleotide sequence of regulator: 798 bp    .

>REG0248 NC_017627:c41877-41080 [Escherichia coli 042]
ATGAAATTAAAACAAAACATCGAAAAAGAGATTATAAAAATCAACAATATCAGAATACATCAGTACACTGTACTATATAC
ATCTAATTGTACAATCGATGTATACACAAAAGAAGGAAGCAATACATATCTTAGAAATGAACTCATATTTCTTGAGAGAG
GAATAAATATATCAGTAAGATTGCAAAAGAAGAAATCAACAGCAAATCCATTTATCGCAATCAGATTAAGCAGCGATACA
TTAAGACGCCTAAAGGATGCCCTGATGATAATATACGGAATATCAAAAGTAGATGCTTGTAGTTGTCCGAATTGGTCAAA
AGGAATAATTGTAGCTGATGCTGACGATTCTGTATTAGATACATTCAAGAGTATCGATAATAATGATGATTCAAGAATTA
CTTCAGATTTGATATATCTTATATCAAAGATCGAAAACAACAAAAAAATTATAGAGTCAATTTATATATCGGCTGTAAGC
TTCTTTTCTGATAAAGTCAGAAACATAATCGAGAAAGACTTATCAAAGAGATGGACTCTAGCTATTATTGCAGATGAATT
TAATGTATCAGAGATAACAATAAGGAAAAGGCTTGAGTCAGAGTATATTACTTTTAACCAGATCCTTATGCAATCAAGAA
TGAGCAAAGCAGCATTGCTGTTGCTTGATAACTCATATCAGATATCACAAATATCTAATATGATAGGATTTTCCAGTACA
TCATATTTCATTAGGCTTTTTGTAAAACATTTTGGCATAACACCAAAACAATTCTTGACTTATTTTAAAAGCCAATAA