Detailed information of Recipient susceptibility factor


Summary

ID RSF000049 experimental
Strain Escherichia coli str. K-12 substr. MG1655
Replicon chromosome
Sequence Protein sequence (211 a.a.); Nucleotide sequence (633 bp)
Response to T6SS attack sensitive
Known or putative function cAMP regulon
Description Both exogenous glucose and a loss of CRP confer protection to attacks by some T6SS effectors. Protection varies with the target and killer strains tested and is mediated by the target cells cyclic adenosine monophosphate (cAMP) receptor protein (CRP) glucose response regulator.
Reference
experimental Experimental investigation has been performed.
External database links

Locus tag (Gene) b3357 (crp)
Coordinates (Strand) 3486120..3486752 (+)
NCBI ID 16131236
RefSeq NC_000913
Uniprot ID CRP_ECOLI
KEGG ID ecj:JW5702, eco:b3357
PDB ID 3N4M, 1O3T, 2WC2, 4HZF, 3HIF, 4I09, 1O3S, 4I0B, 2GZW, 1LB2, 3FWE, 1ZRD, 3RDI, 1I5Z, 3KCC, 1O3R, 1CGP, 3RPQ, 4I0A, 1O3Q, 3QOP, 1HW5, 1ZRE, 4I02, 1G6N, 1J59, 3RYP, 1I6X, 1RUN, 1RUO, 4BHP, 4FT8, 4R8H, 1ZRF, 2CGP, 3RYR, 3ROU, 4I01, 5CIZ, 4BH9, 1ZRC, 3IYD
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
cNMP_binding PF00027.31 3.8e-24 23..109
HTH_Crp_2 PF13545.8 2.8e-13 142..206
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-210 MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTHPDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9929 - -


  Protein sequence: 211 a.a.    .

>RSF000049 NC_000913:3486120-3486752 [Escherichia coli str. K-12 substr. MG1655]
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEG
QERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR*

  Nucleotide sequence: 633 bp    .

>RSF000049 NC_000913:3486120-3486752 [Escherichia coli str. K-12 substr. MG1655]
ATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAA
GAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAG
ACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGC
CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAT
TCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCA
ACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAC
CCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAT
TCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAA