Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00372  |
| Cognate effector | - |
| Related T6SS (Type) | T6SS00551 (Type i3) |
| Strain | Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S |
| Replicon | chromosome |
| Sequence | Protein sequence (139 a.a.); Nucleotide sequence (417 bp) |
| Description | STM14_0336 is toxic when directed to the periplasm of E. coli (pBRA SP-Tlde1) but not to the cytoplasm (pBRA Tlde1), and that STM14_0335 (pEXT22 Tldi1) could neutralize its toxicity |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | STM14_0335 (Tldi1) |
| Coordinates (Strand) | 329200..329616 (+) |
| NCBI ID | 267991976 |
| RefSeq | NC_016856 |
| Uniprot ID | A0A0F6AX89_SALT1 |
| KEGG ID | seo:STM14_0335 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF2195 | PF09961.11 | 3.1e-46 | 8..137 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-4 | VKKY |
| Transmembrane helix | 5-27 | AVEVLFMSACAGMFLPVFAWGGT |
| Outside | 28-138 | DVNIDNPLAECVDIHPVHRQEMDNLTILKTTVTLKKSTGECGCFSTLINYTSLLAQDVEGYGRGSAYSLQEGNISLAKMQGRYPFSFVLSVDNQSVRDQKLALMIRCTPPL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9425 | pos: 23-24. VFA-WG. | VKKYAVEVLFMSACAGMFLPVFA |
Protein sequence: 139 a.a. .
>IMU00372 NC_016856:329200-329616 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]
VKKYAVEVLFMSACAGMFLPVFAWGGTDVNIDNPLAECVDIHPVHRQEMDNLTILKTTVTLKKSTGECGCFSTLINYTSL
LAQDVEGYGRGSAYSLQEGNISLAKMQGRYPFSFVLSVDNQSVRDQKLALMIRCTPPL*
VKKYAVEVLFMSACAGMFLPVFAWGGTDVNIDNPLAECVDIHPVHRQEMDNLTILKTTVTLKKSTGECGCFSTLINYTSL
LAQDVEGYGRGSAYSLQEGNISLAKMQGRYPFSFVLSVDNQSVRDQKLALMIRCTPPL*
Nucleotide sequence: 417 bp .
>IMU00372 NC_016856:329200-329616 [Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S]
GTGAAAAAGTACGCTGTAGAGGTTCTGTTTATGTCTGCCTGTGCAGGAATGTTCCTGCCCGTATTTGCCTGGGGCGGAAC
AGATGTAAACATTGATAACCCGCTGGCGGAGTGTGTGGATATCCATCCCGTTCATCGTCAGGAAATGGATAACCTTACCA
TTCTGAAAACAACGGTCACACTGAAAAAAAGCACCGGTGAATGTGGCTGTTTCTCAACATTAATCAACTATACCAGCTTA
CTGGCGCAGGATGTTGAGGGTTACGGACGCGGAAGTGCATATTCCCTTCAGGAGGGGAATATCTCACTGGCGAAGATGCA
GGGACGTTATCCTTTCAGCTTTGTATTATCGGTGGATAACCAGTCTGTACGGGATCAGAAGCTGGCGCTAATGATACGTT
GTACACCACCGCTGTAA
GTGAAAAAGTACGCTGTAGAGGTTCTGTTTATGTCTGCCTGTGCAGGAATGTTCCTGCCCGTATTTGCCTGGGGCGGAAC
AGATGTAAACATTGATAACCCGCTGGCGGAGTGTGTGGATATCCATCCCGTTCATCGTCAGGAAATGGATAACCTTACCA
TTCTGAAAACAACGGTCACACTGAAAAAAAGCACCGGTGAATGTGGCTGTTTCTCAACATTAATCAACTATACCAGCTTA
CTGGCGCAGGATGTTGAGGGTTACGGACGCGGAAGTGCATATTCCCTTCAGGAGGGGAATATCTCACTGGCGAAGATGCA
GGGACGTTATCCTTTCAGCTTTGTATTATCGGTGGATAACCAGTCTGTACGGGATCAGAAGCTGGCGCTAATGATACGTT
GTACACCACCGCTGTAA