Detailed information of regulator
Summary
| Regulator ID | REG0340  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | TCS regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS01214 (Type Unknown) |
| Strain | Klebsiella pneumoniae strain Kp52.145 |
| Replicon | chromosome |
| Sequence | Protein sequence (48 a.a.); Nucleotide sequence (144 bp) |
| Description | The expression of tssB::lucFF was significantly higher in the h-ns, mgrB and rcsB mutant backgrounds than in the wild-type one and co-incubation of the h-ns, mgrB and rcsB mutants with E. coli in LB resulted in a significant reduction in E. coli survival. |
| Reference |
External database links
| Locus tag (Gene) | BN49_RS29275 (mgrB) |
| Coordinates (Strand) | 3583038..3583181 (-) |
| NCBI ID | 489000713 |
| RefSeq | NZ_FO834906 |
| Uniprot ID | A0A0H3HIQ0_KLEOK, A0A1Q8YNF7_9ENTR, V0B1N8_KLEPN, A0A1C1F5A7_9ENTR, A0A1F2LXV1_9ENTR, A0A1D8JVR0_9ENTR, A0A2S0VK07_9ENTR, A0A264C0F7_KLEVA, MGRB_KLEP3, A0A0G3S8M8_KLEOX, A0A0E4AX29_KLEPN, A0A2W0U461_KLEPN, J6IIL4_9ENTR |
| KEGG ID | kpk:A593_01320, kvd:KR75_25030, ebu:CUC76_24835, kpt:VK055_0108, kom:HR38_22005, kvq:SP68_05400, kll:BJF97_17875, kox:KOX_23700, koc:AB185_18145, kpe:KPK_1949 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| phoPQ | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| MgrB | PF13998.8 | 1.4e-16 | 19..47 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-6 | VKKLRW |
| Transmembrane helix | 7-24 | VLLIVIIAGCLLLWTQML |
| Outside | 25-47 | NVMCDQDVQFFSGICTINKFIPW |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.5550 | pos: 15-16. IAG-CL. | VKKLRWVLLIVIIAG |
Protein sequence of regulator: 48 a.a. .
>REG0340 NZ_FO834906:c3583181-3583038 [Klebsiella pneumoniae]
VKKLRWVLLIVIIAGCLLLWTQMLNVMCDQDVQFFSGICTINKFIPW*
VKKLRWVLLIVIIAGCLLLWTQMLNVMCDQDVQFFSGICTINKFIPW*
Nucleotide sequence of regulator: 144 bp .
>REG0340 NZ_FO834906:c3583181-3583038 [Klebsiella pneumoniae]
GTGAAAAAATTACGGTGGGTTTTACTGATAGTCATCATAGCAGGCTGCCTGTTGCTGTGGACTCAGATGCTTAACGTAAT
GTGCGACCAGGATGTTCAGTTTTTCAGCGGCATTTGCACTATTAATAAATTTATTCCGTGGTAA
GTGAAAAAATTACGGTGGGTTTTACTGATAGTCATCATAGCAGGCTGCCTGTTGCTGTGGACTCAGATGCTTAACGTAAT
GTGCGACCAGGATGTTCAGTTTTTCAGCGGCATTTGCACTATTAATAAATTTATTCCGTGGTAA