Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000004  |
| Strain | Escherichia coli BW25113 strain K-12 |
| Replicon | chromosome |
| Sequence | Protein sequence (280 a.a.); Nucleotide sequence (840 bp) |
| Response to T6SS attack | protective |
| Known or putative function | RcsB regulon response envelope stress |
| Description | The mutants with more-pronounced lysis, and then, competition assays with serial plating confirmed their survival was significantly reduced by the TseL-active T6SS-dependent killer |
| Reference | [1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336 |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | BW25113_RS10750 (wcaA) |
| Coordinates (Strand) | 2126039..2126878 (-) |
| NCBI ID | 446717386 |
| RefSeq | NZ_CP009273 |
| Uniprot ID | WCAA_ECOLI |
| KEGG ID | ecj:JW2044, eco:b2059 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Glycos_transf_2 | PF00535.28 | 2e-28 | 8..165 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-279 | MKNNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQVFTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9844 | - | - |
Protein sequence: 280 a.a. .
>RSF000004 NZ_CP009273:c2126878-2126039 [Escherichia coli BW25113]
MKNNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQA
IMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR*
MKNNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQA
IMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR*
Nucleotide sequence: 840 bp .
>RSF000004 NZ_CP009273:c2126878-2126039 [Escherichia coli BW25113]
ATGAAAAACAATCCGCTGATCTCAATCTATATGCCGACCTGGAACCGCCAACAACTGGCGATTCGGGCAATAAAATCGGT
TTTGCGCCAGGACTACAGCAACTGGGAGATGATCATCGTGGATGATTGCTCCACCTCCTGGGAACAACTGCAACAGTACG
TCACCGCCCTCAACGATCCGCGTATCACTTACATTCACAACGACATTAACAGCGGGGCGTGCGCGGTACGTAACCAGGCG
ATTATGCTGGCGCAAGGGGAATATATCACCGGGATTGATGACGATGACGAATGGACACCCAACCGTCTGAGCGTCTTCCT
CGCCCATAAACAGCAACTGGTCACACACGCCTTTTTGTACGCTAACGACTACGTTTGCCAGGGCGAAGTCTATTCCCAAC
CGGCAAGCTTGCCGCTGTATCCCAAATCGCCGTATTCACGCCGCCTGTTCTACAAACGCAATATCATTGGCAACCAGGTC
TTTACCTGGGCATGGCGTTTCAAAGAGTGTTTGTTCGATACCGAACTGAAAGCGGCACAGGATTACGACATCTTCCTGCG
GATGGTGGTGGAGTACGGCGAACCGTGGAAAGTAGAAGAGGCGACGCAGATCCTGCATATCAATCACGGTGAAATGCAGA
TCACCTCGTCACCGAAAAAATTCTCCGGGTACTTCCACTTTTACCGCAAGCACAAAGACAAATTCGACCGCGCCAGCAAA
AAATATCAGCTGTTTACCCTCTACCAGATCCGCAATAAACGTATGACCTGGCGCACTTTGCTGACGCTGCTGTCGGTGCG
TAACGGCAAACGTCTGGCTGATGGTATTCGGGGGCGCTAA
ATGAAAAACAATCCGCTGATCTCAATCTATATGCCGACCTGGAACCGCCAACAACTGGCGATTCGGGCAATAAAATCGGT
TTTGCGCCAGGACTACAGCAACTGGGAGATGATCATCGTGGATGATTGCTCCACCTCCTGGGAACAACTGCAACAGTACG
TCACCGCCCTCAACGATCCGCGTATCACTTACATTCACAACGACATTAACAGCGGGGCGTGCGCGGTACGTAACCAGGCG
ATTATGCTGGCGCAAGGGGAATATATCACCGGGATTGATGACGATGACGAATGGACACCCAACCGTCTGAGCGTCTTCCT
CGCCCATAAACAGCAACTGGTCACACACGCCTTTTTGTACGCTAACGACTACGTTTGCCAGGGCGAAGTCTATTCCCAAC
CGGCAAGCTTGCCGCTGTATCCCAAATCGCCGTATTCACGCCGCCTGTTCTACAAACGCAATATCATTGGCAACCAGGTC
TTTACCTGGGCATGGCGTTTCAAAGAGTGTTTGTTCGATACCGAACTGAAAGCGGCACAGGATTACGACATCTTCCTGCG
GATGGTGGTGGAGTACGGCGAACCGTGGAAAGTAGAAGAGGCGACGCAGATCCTGCATATCAATCACGGTGAAATGCAGA
TCACCTCGTCACCGAAAAAATTCTCCGGGTACTTCCACTTTTACCGCAAGCACAAAGACAAATTCGACCGCGCCAGCAAA
AAATATCAGCTGTTTACCCTCTACCAGATCCGCAATAAACGTATGACCTGGCGCACTTTGCTGACGCTGCTGTCGGTGCG
TAACGGCAAACGTCTGGCTGATGGTATTCGGGGGCGCTAA