Detailed information of Recipient susceptibility factor


Summary

ID RSF000004 experimental
Strain Escherichia coli BW25113 strain K-12
Replicon chromosome
Sequence Protein sequence (280 a.a.); Nucleotide sequence (840 bp)
Response to T6SS attack protective
Known or putative function RcsB regulon response envelope stress
Description The mutants with more-pronounced lysis, and then, competition assays with serial plating confirmed their survival was significantly reduced by the TseL-active T6SS-dependent killer
Reference
experimental Experimental investigation has been performed.

External database links

Locus tag (Gene) BW25113_RS10750 (wcaA)
Coordinates (Strand) 2126039..2126878 (-)
NCBI ID 446717386
RefSeq NZ_CP009273
Uniprot ID WCAA_ECOLI
KEGG ID ecj:JW2044, eco:b2059
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Glycos_transf_2 PF00535.28 2e-28 8..165

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-279 MKNNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQVFTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9844 - -



  Protein sequence: 280 a.a.    .

>RSF000004 NZ_CP009273:c2126878-2126039 [Escherichia coli BW25113]
MKNNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQA
IMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR*

  Nucleotide sequence: 840 bp    .

>RSF000004 NZ_CP009273:c2126878-2126039 [Escherichia coli BW25113]
ATGAAAAACAATCCGCTGATCTCAATCTATATGCCGACCTGGAACCGCCAACAACTGGCGATTCGGGCAATAAAATCGGT
TTTGCGCCAGGACTACAGCAACTGGGAGATGATCATCGTGGATGATTGCTCCACCTCCTGGGAACAACTGCAACAGTACG
TCACCGCCCTCAACGATCCGCGTATCACTTACATTCACAACGACATTAACAGCGGGGCGTGCGCGGTACGTAACCAGGCG
ATTATGCTGGCGCAAGGGGAATATATCACCGGGATTGATGACGATGACGAATGGACACCCAACCGTCTGAGCGTCTTCCT
CGCCCATAAACAGCAACTGGTCACACACGCCTTTTTGTACGCTAACGACTACGTTTGCCAGGGCGAAGTCTATTCCCAAC
CGGCAAGCTTGCCGCTGTATCCCAAATCGCCGTATTCACGCCGCCTGTTCTACAAACGCAATATCATTGGCAACCAGGTC
TTTACCTGGGCATGGCGTTTCAAAGAGTGTTTGTTCGATACCGAACTGAAAGCGGCACAGGATTACGACATCTTCCTGCG
GATGGTGGTGGAGTACGGCGAACCGTGGAAAGTAGAAGAGGCGACGCAGATCCTGCATATCAATCACGGTGAAATGCAGA
TCACCTCGTCACCGAAAAAATTCTCCGGGTACTTCCACTTTTACCGCAAGCACAAAGACAAATTCGACCGCGCCAGCAAA
AAATATCAGCTGTTTACCCTCTACCAGATCCGCAATAAACGTATGACCTGGCGCACTTTGCTGACGCTGCTGTCGGTGCG
TAACGGCAAACGTCTGGCTGATGGTATTCGGGGGCGCTAA