Detailed information of regulator
Summary
| Regulator ID | REG0343  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS01214 (Type Unknown) |
| Strain | Klebsiella pneumoniae strain Kp52.145 |
| Replicon | chromosome |
| Sequence | Protein sequence (151 a.a.); Nucleotide sequence (453 bp) |
| Description | The expression of tssB::lucFF was significantly higher in the h-ns, mgrB and rcsB mutant backgrounds than in the wild-type one and E. coli was not killed when co-incubated with rpoN, rpoS, hfq, fur and pmrAB mutants. |
| Reference |
External database links
| Locus tag (Gene) | BN49_RS10300 (fur) |
| Coordinates (Strand) | 1826878..1827330 (-) |
| NCBI ID | 514077307 |
| RefSeq | NZ_FO834906 |
| Uniprot ID | W9BF75_KLEPN |
| KEGG ID | kpnk:BN49_1760 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| TssB transcription | ↓ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| FUR | PF01475.21 | 1.9e-48 | 10..130 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-150 | MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLIDMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEFSDDSIELRQREIASRHGIRLTNHSLYLYGHCAEGDCREDEHAHDVVEK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9976 | - | - |
Protein sequence of regulator: 151 a.a. .
>REG0343 NZ_FO834906:c1827330-1826878 [Klebsiella pneumoniae]
MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLIDMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVF
ELTQQHHHDHLICLDCGKVIEFSDDSIELRQREIASRHGIRLTNHSLYLYGHCAEGDCREDEHAHDVVEK*
MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLIDMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVF
ELTQQHHHDHLICLDCGKVIEFSDDSIELRQREIASRHGIRLTNHSLYLYGHCAEGDCREDEHAHDVVEK*
Nucleotide sequence of regulator: 453 bp .
>REG0343 NZ_FO834906:c1827330-1826878 [Klebsiella pneumoniae]
ATGACTGACAACAATACCGCATTAAAGAAGGCTGGCCTGAAAGTCACACTTCCTCGACTAAAAATCCTTGAAGTGCTGCA
GGAACCGGATAACCATCATGTCAGTGCGGAAGACTTATATAAACGCCTGATCGACATGGGCGAAGAGATCGGTCTGGCGA
CCGTTTACCGCGTCCTCAACCAGTTTGATGACGCCGGCATCGTGACTCGTCATAATTTCGAAGGCGGAAAATCCGTTTTC
GAACTGACCCAGCAGCATCACCACGATCACCTGATCTGCCTCGACTGCGGCAAAGTGATCGAATTCAGCGACGACTCGAT
CGAATTACGCCAGCGTGAAATTGCCTCCCGCCACGGCATCCGCCTGACCAACCACAGTTTATATCTGTACGGTCACTGTG
CGGAAGGGGATTGCCGCGAGGACGAACACGCCCACGACGTGGTGGAAAAATAA
ATGACTGACAACAATACCGCATTAAAGAAGGCTGGCCTGAAAGTCACACTTCCTCGACTAAAAATCCTTGAAGTGCTGCA
GGAACCGGATAACCATCATGTCAGTGCGGAAGACTTATATAAACGCCTGATCGACATGGGCGAAGAGATCGGTCTGGCGA
CCGTTTACCGCGTCCTCAACCAGTTTGATGACGCCGGCATCGTGACTCGTCATAATTTCGAAGGCGGAAAATCCGTTTTC
GAACTGACCCAGCAGCATCACCACGATCACCTGATCTGCCTCGACTGCGGCAAAGTGATCGAATTCAGCGACGACTCGAT
CGAATTACGCCAGCGTGAAATTGCCTCCCGCCACGGCATCCGCCTGACCAACCACAGTTTATATCTGTACGGTCACTGTG
CGGAAGGGGATTGCCGCGAGGACGAACACGCCCACGACGTGGTGGAAAAATAA