SecReT6, T6SS database

Detailed information of Recipient susceptibility factor

Summary

ID	RSF000027
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (475 a.a.); Nucleotide sequence (1425 bp)
Response to T6SS attack	protective
Known or putative function	Maintaining envelope integrity
Description	Maintaining envelope integrity was important for surviving TseH attacks, and deletion periplasmic protease and chaperone DegP involved in this process showed impaired survival when challenged with the TseH WT strain.
Reference	[1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [3] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

External database links

Locus tag (Gene)	BW25113_RS00810 (degP)
Coordinates (Strand)	177371..178795 (+)
NCBI ID	446676600
RefSeq	NZ_CP009273
Uniprot ID	M9G2M0_ECOLX, L3BWD5_ECOLX, S1EBK7_ECOLX, L3QHT5_ECOLX, S1IXD5_ECOLX, L4JG49_ECOLX, U9XL22_ECOLX, U9XSD1_ECOLX, V0A2W1_ECOLX, V0Z4C3_ECOLX, U9ZII1_ECOLX, A0A080JBX1_ECOLX, E3PDB4_ECOH1, A0A0E1SVN9_ECOLX, A0A140NAV6_ECOBD, A0A0H3PJI5_ECO5C, D6IID4_ECOLX, D6J624_ECOLX, F4NQV4_ECOLX, C3TPR2_ECOLX, E0IYM0_ECOLW, B7N830_ECOLU, A0A237FWH1_SHIBO, A0A1Q8NRY0_SHIDY, A0A0I2TNG1_SHISO, A0A1X3JNL6_ECOLX, A0A1X3LBR7_ECOLX, F4VNQ3_ECOLX, F4SFG6_ECOLX, A0A1X3IC92_ECOLX, A0A0K9TI34_ECOLX, F4T9Z7_ECOLX, A0A1X3L993_ECOLX, A0A1X3J7X5_ECOLX, D3QWG3_ECOCB, A0A0E1LV58_ECOLX, D7Y754_ECOM1, I4T698_ECOLX, DEGP_ECOLI, DEGP_ECO57, I2SKX7_ECOLX
KEGG ID	ece:Z0173, ebd:ECBD_3457, elw:ECW_m0158, eco:b0161, eum:ECUMN_0159, ecj:JW0157, eok:G2583_0165, elh:ETEC_0157, ecs:ECs0165
PDB ID	6JJK, 6JJO, 2ZLE, 3CS0, 6JJL, 3MH5, 3MH6, 3OU0, 1KY9, 3OTP, 3MH4, 3MH7, 4A8D

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Trypsin_2	PF13365.8	3.8e-31	114..250
PDZ_2	PF13180.8	1.8e-15	297..380
PDZ	PF00595.26	4.2e-12	393..463

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-474	MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGNGGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRSDIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNAENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGDVITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSSIFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKVLDSKPSVLALNIQRGDSTIYLLMQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
SP(Sec/SPI)	0.9971	pos: 26-27. ATA-AE.	MKKTTLALSALALSLGLALSPLSATA

Protein sequence: 475 a.a. .

                                    >RSF000027 NZ_CP009273:177371-178795 [Escherichia coli BW25113]
MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDS
PFCQEGSPFQSSPFCQGGQGGNGGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNAENYENFIQTDAAINRGNSGGAL
VNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVL
PNSSAAKAGIKAGDVITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSSIFNGIEGA
EMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKVLDSKPSVLALNIQRGDSTIYLLMQ*

                                

Nucleotide sequence: 1425 bp .

                                    >RSF000027 NZ_CP009273:177371-178795 [Escherichia coli BW25113]
ATGAAAAAAACCACATTAGCACTGAGTGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGC
TGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGATGCTCGAAAAGGTGATGCCTTCAGTGGTCA
GCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCT
CCGTTCTGCCAGGAAGGTTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCA
ACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCTATGTCGTCACCAACAACCACGTTGTTG
ATAACGCGACGGTCATTAAAGTTCAACTGAGCGATGGCCGTAAGTTCGACGCGAAGATGGTTGGCAAAGATCCGCGCTCT
GATATCGCGCTGATCCAAATCCAGAACCCGAAAAACCTGACCGCAATTAAGATGGCGGATTCTGATGCACTGCGCGTGGG
TGATTACACCGTAGCGATTGGTAACCCGTTTGGTCTGGGCGAGACGGTAACTTCCGGGATTGTCTCTGCGCTGGGGCGTA
GCGGCCTGAATGCCGAAAACTACGAAAACTTCATCCAGACCGATGCAGCGATCAACCGTGGTAACTCCGGTGGTGCGCTG
GTTAACCTGAACGGCGAACTGATCGGTATCAACACCGCGATCCTCGCACCGGACGGCGGCAACATCGGTATCGGTTTTGC
TATCCCGAGTAACATGGTGAAAAACCTGACCTCGCAGATGGTGGAATACGGCCAGGTGAAACGCGGTGAGCTGGGTATTA
TGGGGACTGAGCTGAACTCCGAACTGGCGAAAGCGATGAAAGTTGACGCCCAGCGCGGTGCTTTCGTAAGCCAGGTTCTG
CCTAATTCCTCCGCTGCAAAAGCGGGCATTAAAGCGGGTGATGTGATCACCTCACTGAACGGTAAGCCGATCAGCAGCTT
TGCCGCACTGCGTGCTCAGGTGGGTACTATGCCGGTAGGCAGCAAACTGACCCTGGGCTTACTGCGCGACGGTAAGCAGG
TTAACGTGAACCTGGAACTGCAGCAGAGCAGCCAGAATCAGGTTGATTCCAGCTCCATCTTCAACGGCATTGAAGGCGCT
GAGATGAGCAACAAAGGCAAAGATCAGGGCGTGGTAGTGAACAACGTGAAAACGGGCACTCCGGCTGCGCAGATCGGCCT
GAAGAAAGGTGATGTGATTATTGGCGCGAACCAGCAGGCAGTGAAAAACATCGCTGAACTGCGTAAAGTTCTCGACAGCA
AACCGTCTGTGCTGGCACTCAACATTCAGCGCGGCGACAGCACCATCTACCTGTTAATGCAGTAA

                                