Detailed information of Recipient susceptibility factor

Summary

ID	RSF000030
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (208 a.a.); Nucleotide sequence (624 bp)
Response to T6SS attack	protective
Known or putative function	RcsB regulon response envelope stress
Description	Overexpression of rcsA protected E. coli by up to 10,000-fold compared with the vector-only control against the T6SS of WT V. cholerae V52 with all four antibacterial effec- tors intact and active and the protection did not occur in the ΔwcaJ background.
Reference	[1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [3] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

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External database links

Locus tag (Gene)	BW25113_RS10200 (RcsA)
Coordinates (Strand)	2017449..2018072 (+)
NCBI ID	446026146
RefSeq	NZ_CP009273
Uniprot ID	K4WRU6_ECOLX, L4J9U1_ECOLX, U9YVJ7_ECOLX, U9Z1S1_ECOLX, V0ZA12_ECOLX, A0A0E2LIY6_ECOU3, E3PBD7_ECOH1, A0A0H2YZR7_ECOK1, C3T4Q7_ECOLX, A0A170UEY3_KLEOX, B7MCK7_ECO45, B7NBU4_ECOLU, A0A1X3KCA0_ECOLX, A0A0K9T5N9_ECOLX, F4TFT6_ECOLX, A0A1X3LZG6_ECOLX, E9XIP6_ECOLX, D8E6L5_ECOLX, D3QWX2_ECOCB, RCSA_ECOLI, RCSA_ECO57
KEGG ID	eum:ECUMN_2243, eco:b1951, elh:ETEC_2054, ecz:ECS88_2004, eok:G2583_2402, ecj:JW1935, ecs:ECs2690, ecv:APECO1_990, ece:Z3041
PDB ID	-

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Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GerE	PF00196.21	4.3e-19	137..191

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Transmembrane helices

• Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-207	MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACDSQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLLISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISDQMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR

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Signal peptides

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9799	-	-

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  Protein sequence: 208 a.a.    .Download


  Nucleotide sequence: 624 bp    .Download