Detailed information of Recipient susceptibility factor


Summary

ID RSF000056 experimental
Strain Klebsiella pneumoniae subsp. pneumoniae BJ1-GA
Replicon chromosome
Sequence Protein sequence (379 a.a.); Nucleotide sequence (1137 bp)
Response to T6SS attack protective
Known or putative function capsule protection
Description Group I capsules confer a strong protection from V. cholerae's T6SS attacks to commensal Klebsiella strains.
Reference
experimental Experimental investigation has been performed.
External database links

Locus tag (Gene) KPST380_RS07600 (wza)
Coordinates (Strand) 3683821..3684957 (-)
NCBI ID WP_004180522.1
RefSeq NZ_HG518479
Uniprot ID A0A7U7IJY4_KLEPN, YC04_KLEPN, C9K1K1_KLEPN, A0A4S6LZ82_KLEPN
KEGG ID kpnk:BN49_3727, kpt:VK055_5015
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Poly_export PF02563.18 6.8e-28 82..165
Wza_C PF18412.3 1.8e-17 345..374
SLBB PF10531.11 1.2e-08 173..221
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MKKK
Transmembrane helix 5-27 IVRFSALALAIGFLSGCTIVPGQ
Outside 28-378 GLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPETILARPNPQLDNLLRSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSQVRQDIASRLTTYIESPQVDVSVAAFRSQKAYVTGEVAKSGQQPITNIPLTVMDAINAAGGLAPDADWRNVVLTHNGKDTKVSLYALMQKGDLTQNHLLYPGDILFVPRNDDLKVFVMGEVGKQSTLKMDRSGMTIAEALGNAEGMSQAFSDATGVFVIRQLKGDKQGKIANIYQLNAQDASAMVLGTEFQLQPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETAKFIKDWP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9981 pos: 20-21. LSG-CT. MKKKIVRFSALALAIGFLSG


  Protein sequence: 379 a.a.    .

>RSF000056 NZ_HG518479:c3684957-3683821 [Klebsiella pneumoniae subsp. pneumoniae BJ1-GA]
MKKKIVRFSALALAIGFLSGCTIVPGQGLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPETILARPNPQLDNLL
RSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSQVRQDIASRLTTYIESPQ
VDVSVAAFRSQKAYVTGEVAKSGQQPITNIPLTVMDAINAAGGLAPDADWRNVVLTHNGKDTKVSLYALMQKGDLTQNHL
LYPGDILFVPRNDDLKVFVMGEVGKQSTLKMDRSGMTIAEALGNAEGMSQAFSDATGVFVIRQLKGDKQGKIANIYQLNA
QDASAMVLGTEFQLQPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETAKFIKDWP*

  Nucleotide sequence: 1137 bp    .

>RSF000056 NZ_HG518479:c3684957-3683821 [Klebsiella pneumoniae subsp. pneumoniae BJ1-GA]
ATGAAGAAAAAAATTGTTAGATTTTCGGCATTAGCATTGGCGATTGGGTTTTTATCGGGTTGTACCATTGTCCCTGGTCA
GGGATTAAACAGTCTACGTAAGAACGTGGTGGAGCTTCCAGACAGTGATTACGATTTGGATAAACTGGTCAATGTGTACC
CGATGACGCCGGGGCTTATCGATCAGCTTCGACCGGAAACCATACTCGCTCGTCCAAACCCGCAGCTGGATAATTTACTA
CGAAGTTATGAATATCGCATTGGGGTTGGCGATGTATTAATGGTAACCGTTTGGGATCACCCGGAGCTGACAACACCTGC
AGGTCAGTACCGTAGCGCCAGTGACACCGGTAACTGGGTGAATTCTGATGGAACCATTTTCTATCCCTATATAGGTAAGG
TGCAGGTGGCGGGCAAGACGCTTAGCCAGGTACGTCAGGATATAGCCAGTCGTTTGACGACTTATATTGAAAGTCCCCAG
GTTGATGTAAGTGTTGCAGCATTCCGTTCGCAAAAAGCCTATGTAACTGGTGAAGTTGCAAAATCCGGGCAGCAACCGAT
AACTAATATTCCATTAACCGTAATGGACGCTATCAATGCTGCAGGTGGTCTTGCACCAGATGCTGACTGGCGAAATGTTG
TGTTAACGCATAATGGTAAAGATACTAAAGTTTCTTTGTACGCTTTGATGCAGAAAGGGGACTTGACACAAAATCATTTA
CTTTATCCTGGGGATATTCTTTTTGTCCCACGCAATGATGATTTAAAAGTATTTGTCATGGGGGAAGTGGGTAAACAAAG
TACGCTAAAAATGGACCGCAGTGGTATGACAATTGCTGAAGCACTAGGCAATGCTGAAGGTATGTCGCAAGCGTTCAGTG
ATGCAACTGGAGTATTTGTTATTCGCCAGTTGAAAGGTGACAAGCAAGGTAAAATAGCTAACATTTATCAGCTTAATGCA
CAAGATGCTTCGGCAATGGTTCTGGGAACAGAATTTCAATTGCAACCATATGATATTGTATATGTCACGACAGCTCCTTT
AGTACGTTGGAATCGTGTAATTTCTCAGCTTGTCCCGACTATTACCGGCGTTCATGACATGACGGAAACTGCGAAATTTA
TTAAGGATTGGCCATAA