Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00328  |
| Cognate effector | EFF01527 |
| Related T6SS (Type) | T6SS00947 (Type Unknown) |
| Strain | Escherichia coli strain PE050 |
| Replicon | chromosome |
| Sequence | Protein sequence (150 a.a.); Nucleotide sequence (450 bp) |
| Description | ETEC PE050 cells deploy VT1 by T6SS to inhibit other cells and protect themselves with immunity protein VTI1 |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | vt6C (VTI1) |
| Coordinates (Strand) | 3177..3626 (+) |
| NCBI ID | 1062685203 |
| GenBank | KX118292 |
| Uniprot ID | A0A0J2B9J7_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-149 | MFMNMIKLVIKQKVKALISIVLINFFFCSNSYSEEKIEIININKPIYIGTNQSDKVLITACNHWVLNSRDIIDIFNLSIKHDDYGEINRMYYWLPCEIRGRLMYDGIEWSFVINAAATAMWMSNDKEIYWGCNKKACDNFFLLPYDGMR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.6052 | pos: 33-34. SYS-EE. | MFMNMIKLVIKQKVKALISIVLINFFFCSNSYS |
Protein sequence: 150 a.a. .
>IMU00328 KX118292:3177-3626 [Escherichia coli]
MFMNMIKLVIKQKVKALISIVLINFFFCSNSYSEEKIEIININKPIYIGTNQSDKVLITACNHWVLNSRDIIDIFNLSIK
HDDYGEINRMYYWLPCEIRGRLMYDGIEWSFVINAAATAMWMSNDKEIYWGCNKKACDNFFLLPYDGMR*
MFMNMIKLVIKQKVKALISIVLINFFFCSNSYSEEKIEIININKPIYIGTNQSDKVLITACNHWVLNSRDIIDIFNLSIK
HDDYGEINRMYYWLPCEIRGRLMYDGIEWSFVINAAATAMWMSNDKEIYWGCNKKACDNFFLLPYDGMR*
Nucleotide sequence: 450 bp .
>IMU00328 KX118292:3177-3626 [Escherichia coli]
ATGTTTATGAACATGATAAAATTAGTTATAAAACAGAAGGTGAAGGCGCTGATCTCTATAGTGTTAATTAATTTCTTTTT
TTGTTCAAATAGTTACTCCGAAGAAAAAATAGAGATAATAAACATTAATAAACCAATATATATAGGTACCAATCAATCAG
ATAAGGTGCTTATTACTGCGTGTAATCATTGGGTGCTTAATTCTAGAGATATTATAGACATATTCAATCTAAGTATAAAG
CATGATGATTATGGCGAAATAAACAGAATGTATTATTGGTTACCTTGTGAAATTAGAGGGAGACTAATGTATGACGGAAT
AGAATGGAGTTTTGTTATCAATGCTGCTGCAACAGCAATGTGGATGAGCAATGATAAAGAGATTTACTGGGGATGCAATA
AAAAAGCATGCGATAATTTCTTTTTATTACCATATGATGGGATGAGGTAA
ATGTTTATGAACATGATAAAATTAGTTATAAAACAGAAGGTGAAGGCGCTGATCTCTATAGTGTTAATTAATTTCTTTTT
TTGTTCAAATAGTTACTCCGAAGAAAAAATAGAGATAATAAACATTAATAAACCAATATATATAGGTACCAATCAATCAG
ATAAGGTGCTTATTACTGCGTGTAATCATTGGGTGCTTAATTCTAGAGATATTATAGACATATTCAATCTAAGTATAAAG
CATGATGATTATGGCGAAATAAACAGAATGTATTATTGGTTACCTTGTGAAATTAGAGGGAGACTAATGTATGACGGAAT
AGAATGGAGTTTTGTTATCAATGCTGCTGCAACAGCAATGTGGATGAGCAATGATAAAGAGATTTACTGGGGATGCAATA
AAAAAGCATGCGATAATTTCTTTTTATTACCATATGATGGGATGAGGTAA