Detailed information of effector
Summary
| Effector ID | EFF01527  |
| Effector type | Tae |
| Cognate immunity protein | IMU00328 |
| Related T6SS (Type) | T6SS00947 (Type Unknown) |
| Strain | Escherichia coli strain PE050 |
| Replicon | chromosome |
| Sequence | Protein sequence (218 a.a.); Nucleotide sequence (654 bp) |
| Description | VT1 is a novel amidase effector for peptidoglycan degradation of target cell walls and a significant decrease in E. coli survival and optical density when periplasmic localized VT1 (Peri-VT1) |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | vt6B (VT1) |
| Coordinates (Strand) | 2514..3167 (+) |
| NCBI ID | 1062685203 |
| GenBank | KX118292 |
| Uniprot ID | A0A0J2B9J7_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Amidase_2 | PF01510.27 | 6.9e-12 | 77..210 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-217 | MSKNVILSNGERENPSPLIYDKHKKHCIFRTKTLLSEEKKEDEKCTVSVVFIDHEGYIRNAGIIKHTVPELEEGRMPKINAIILHRTDSSTINGTLASFSKGIGAHFIVEKDGNIYQAASLFVYTSHVGKIKSRCKEEKTWSSEEKKKIEAFGWNAKKIHDHEKIKQYPYRYPYNMDSVGIEVIALYDKKNKKWESATDSQKKSIEKLVDIIKIFMS |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9928 | - | - |
Protein sequence: 218 a.a. .
>EFF01527 KX118292:2514-3167 Type VI Amidase Effector (Tae) [Escherichia coli]
MSKNVILSNGERENPSPLIYDKHKKHCIFRTKTLLSEEKKEDEKCTVSVVFIDHEGYIRNAGIIKHTVPELEEGRMPKIN
AIILHRTDSSTINGTLASFSKGIGAHFIVEKDGNIYQAASLFVYTSHVGKIKSRCKEEKTWSSEEKKKIEAFGWNAKKIH
DHEKIKQYPYRYPYNMDSVGIEVIALYDKKNKKWESATDSQKKSIEKLVDIIKIFMS*
MSKNVILSNGERENPSPLIYDKHKKHCIFRTKTLLSEEKKEDEKCTVSVVFIDHEGYIRNAGIIKHTVPELEEGRMPKIN
AIILHRTDSSTINGTLASFSKGIGAHFIVEKDGNIYQAASLFVYTSHVGKIKSRCKEEKTWSSEEKKKIEAFGWNAKKIH
DHEKIKQYPYRYPYNMDSVGIEVIALYDKKNKKWESATDSQKKSIEKLVDIIKIFMS*
Nucleotide sequence: 654 bp .
>EFF01527 KX118292:2514-3167 Type VI Amidase Effector (Tae) [Escherichia coli]
ATGAGCAAAAATGTAATATTAAGTAATGGGGAAAGAGAAAATCCTTCTCCGCTGATCTATGACAAGCATAAAAAGCACTG
TATTTTTCGTACAAAGACATTACTTAGTGAAGAGAAAAAAGAAGACGAAAAATGTACTGTATCAGTTGTATTTATTGATC
ATGAGGGGTATATAAGAAATGCTGGAATTATAAAACACACAGTCCCTGAACTTGAAGAAGGTCGCATGCCTAAAATTAAT
GCAATAATATTGCATAGAACAGATTCTTCAACAATAAATGGTACACTTGCTAGTTTTTCTAAAGGCATTGGAGCTCATTT
TATTGTTGAAAAAGATGGTAATATATACCAGGCTGCAAGCCTATTTGTATATACCAGCCATGTGGGGAAGATAAAGTCTC
GATGTAAGGAGGAAAAAACATGGTCGTCTGAAGAAAAAAAGAAAATAGAGGCATTCGGATGGAATGCTAAGAAGATACAT
GATCATGAAAAAATTAAACAATATCCTTATAGATATCCATATAATATGGACAGTGTTGGCATTGAGGTTATTGCATTATA
TGATAAAAAAAATAAAAAATGGGAGTCTGCTACAGACTCTCAAAAAAAATCAATTGAGAAACTTGTAGATATAATAAAAA
TATTTATGAGTTAG
ATGAGCAAAAATGTAATATTAAGTAATGGGGAAAGAGAAAATCCTTCTCCGCTGATCTATGACAAGCATAAAAAGCACTG
TATTTTTCGTACAAAGACATTACTTAGTGAAGAGAAAAAAGAAGACGAAAAATGTACTGTATCAGTTGTATTTATTGATC
ATGAGGGGTATATAAGAAATGCTGGAATTATAAAACACACAGTCCCTGAACTTGAAGAAGGTCGCATGCCTAAAATTAAT
GCAATAATATTGCATAGAACAGATTCTTCAACAATAAATGGTACACTTGCTAGTTTTTCTAAAGGCATTGGAGCTCATTT
TATTGTTGAAAAAGATGGTAATATATACCAGGCTGCAAGCCTATTTGTATATACCAGCCATGTGGGGAAGATAAAGTCTC
GATGTAAGGAGGAAAAAACATGGTCGTCTGAAGAAAAAAAGAAAATAGAGGCATTCGGATGGAATGCTAAGAAGATACAT
GATCATGAAAAAATTAAACAATATCCTTATAGATATCCATATAATATGGACAGTGTTGGCATTGAGGTTATTGCATTATA
TGATAAAAAAAATAAAAAATGGGAGTCTGCTACAGACTCTCAAAAAAAATCAATTGAGAAACTTGTAGATATAATAAAAA
TATTTATGAGTTAG