Detailed information of Recipient susceptibility factor

Summary

ID	RSF000019
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (217 a.a.); Nucleotide sequence (651 bp)
Response to T6SS attack	protective
Known or putative function	anti-sigma-E factor
Description	When co-incubated with T6SS-active 4effc compared with the ΔvasK mutant, whereas the tolB mutant showed considerable cell lysis but a nonsignificant difference between 4effc and ΔvasK. Competition assays show that survival of tolB and rseA was significantly reduced by 4effc.
Reference	[1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

---

External database links

Locus tag (Gene)	BW25113_RS13420 (rseA)
Coordinates (Strand)	2702113..2702763 (-)
NCBI ID	447091203
RefSeq	NZ_CP009273
Uniprot ID	M9FVC8_ECOLX, A0A0E0XXB1_ECO1C, K4W8J3_ECOLX, S1ESU4_ECOLX, L3Q147_ECOLX, S1ICN2_ECOLX, L4J474_ECOLX, U9YQ82_ECOLX, U9XME1_ECOLX, V0SQF3_ECOLX, U9ZQ44_ECOLX, V0VL40_ECOLX, V0Z1I6_ECOLX, U9ZJM7_ECOLX, A0A080IUC7_ECOLX, A0A0A0FD10_ECOLX, RSEA_ECOL6, D3H3T2_ECO44, Q32CU9_SHIDS, E3PGW2_ECOH1, A7ZQ16_ECO24, A0A0E1SVJ2_ECOLX, Q1R8G0_ECOUT, B6I5E6_ECOSE, B1LP85_ECOSM, A0A140N9I3_ECOBD, J7RJQ4_ECOLX, D6IA08_ECOLX, D6JD47_ECOLX, F4NGK2_ECOLX, E2QQ32_ECOLX, E0J4I8_ECOLW, A0A181WNC0_KLEOX, B7UH12_ECO27, B7LUZ2_ESCF3, B7LDG5_ECO55, B7N6G0_ECOLU, A0A2S8D9Q1_SHIDY, A0A0K9TE70_ECOLX, A0A1X3LGM9_ECOLX, A0A1X3M0T3_ECOLX, E9XI67_ECOLX, E6BH03_ECOLX, E1IN63_ECOLX, E1J3R0_ECOLX, D8E295_ECOLX, A0A0H3EJX9_ECO8N, A0A0E1M0C2_ECOLX, D7XMV2_ECOLX, D7Y9T8_ECOM1, E9TC10_ECOLX, I4T734_ECOLX, RSEA_ECOLI, I2USR9_ECOLX, I2WVG3_ECOLX, W9AIN5_ECOLX
KEGG ID	eum:ECUMN_2894, elw:ECW_m2801, ecm:EcSMS35_2725, eci:UTI89_C2894, ecj:JW2556, eco:b2572, ecg:E2348C_2849, efe:EFER_0502, ecw:EcE24377A_2858, esl:O3K_06550, eln:NRG857_12770, ecos:EC958_2878, sdy:SDY_2813, elh:ETEC_2785, ecc:c3096, ecy:ECSE_2860, ebd:ECBD_1109, elo:EC042_2776, eck:EC55989_2861
PDB ID	1OR7, 1YFN, 3M4W

---

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
RseA_N	PF03872.15	1.1e-36	1..87
RseA_C	PF03873.15	1.5e-25	132..186

---

Transmembrane helices

• Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-216	MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMRGDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLTQMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANNGQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ

---

Signal peptides

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9946	-	-

---

  Protein sequence: 217 a.a.    .Download


  Nucleotide sequence: 651 bp    .Download