Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00935 experimental
T6SS type Type i2
Strain Escherichia coli 17-2
Replicon (RefSeq) chromosome (NZ_JACEFV010000001)
Location 909481..942047
Function anti-eukaryotic; effector translocation
Comment sci-1 gene cluster is involved in biofilm formation.
References
[1] Aschtgen MS et al (2008) SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coli. J Bacteriol. 190(22):7523-31. [PudMed:18805985]
[2] Zoued A et al (2013) TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the Type VI secretion system. J Biol Chem. 288(38):27031-41. [PudMed:23921384]
[3] Nguyen VS et al (2015) Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody. PLoS One. 10(3):e0122187. [PudMed:25811612]
[4] Durand E et al (2012) Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. J Biol Chem. 287(17):14157-68. [PudMed:22371492]
[5] Aschtgen MS et al (2012) The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC. Microbiologyopen. 1(1):71-82. [PudMed:22950014]
[6] Felisberto-Rodrigues C et al (2011) Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 7(11):e1002386. [PudMed:22102820]
[7] Aschtgen MS et al (2010) The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wall. Mol Microbiol. 75(4):886-99. [PudMed:20487285]
[8] Brunet YR et al (2013) Imaging Type VI Secretion-Mediated Bacterial Killing. Cell Rep. 3(1):36-41. [PudMed:23291094]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssL
Number211111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
H1D38_RS04315 904569..904892 - 324 485761811 hypothetical protein
H1D38_RS04320 904958..905602 - 645 485761812 hypothetical protein
H1D38_RS04325 905623..905892 - 270 446148662 PerC family transcriptional regulator
H1D38_RS04330 905971..906615 - 645 446425008 ParB N-terminal domain-containing protein
H1D38_RS04335 906600..907832 - 1233 447127386 phosphoadenosine phosphosulfate reductase
H1D38_RS04340 907873..908703 - 831 446293040 hypothetical protein
H1D38_RS04345 908706..909266 - 561 446508647 hypothetical protein
T6CP016069 TssA H1D38_RS04350 909481..910848 - 1368 446122356 type VI secretion system ImpA family N-terminal domain-containing protein
H1D38_RS04355 910903..911208 - 306 485799642 hypothetical protein
H1D38_RS04360 911273..913018 - 1746 485765580 AAA family ATPase
H1D38_RS04365 913254..914504 - 1251 446819774 GIY-YIG nuclease family protein
H1D38_RS04370 914660..915061 - 402 485781047 hypothetical protein
T6CP016070 TssE H1D38_RS04375 (tssE) 915218..915649 - 432 447060676 type VI secretion system baseplate subunit TssE
T6CP016071 TssJ H1D38_RS04380 (tssJ) 915652..916188 - 537 446897213 type VI secretion system lipoprotein TssJ
T6CP016072 TssG H1D38_RS04385 (tssG) 916169..917269 - 1101 446475927 type VI secretion system baseplate subunit TssG
T6CP016073 TssF H1D38_RS04390 (tssF) 917224..918987 - 1764 446264608 type VI secretion system baseplate subunit TssF
H1D38_RS04395 918995..919687 - 693 446037502 hypothetical protein
T6CP016074 TssA H1D38_RS04400 (tssA) 919689..921287 - 1599 446068660 type VI secretion system protein TssA
H1D38_RS04405 921287..924676 - 3390 446959833 type VI secretion protein VasK
H1D38_RS04410 924669..925817 - 1149 485781045 hypothetical protein
H1D38_RS04415 925821..926087 - 267 445955555 PAAR domain-containing protein
H1D38_RS04420 (tli1) 926119..926795 - 677 pseudogene T6SS immunity phospholipase A1-binding lipoprotein Tli1-EAEC
H1D38_RS04425 (tli1) 926939..927615 - 677 pseudogene T6SS immunity phospholipase A1-binding lipoprotein Tli1-EAEC
H1D38_RS04430 (tle1) 927635..929317 - 1683 446092701 T6SS effector phospholipase Tle1-EAEC
T6CP016075 TssI H1D38_RS04435 (vgrG) 929314..931839 - 2526 446977247 type VI secretion system tip protein VgrG
H1D38_RS04440 931826..932368 - 543 485781044 hypothetical protein
H1D38_RS04445 932362..932928 - 567 446070506 hypothetical protein
T6CP016076 TssH H1D38_RS04450 (tssH) 932925..935582 - 2658 446353791 type VI secretion system ATPase TssH
T6CP016077 TssD H1D38_RS04455 (hcp) 935751..936242 - 492 446930057 type VI secretion system effector Hcp
H1D38_RS04460 936248..937978 - 1731 446480629 OmpA family protein
T6CP016078 TssL H1D38_RS04465 (tssL) 937981..938634 - 654 446962789 type VI secretion system protein TssL, short form
T6CP016079 TssK H1D38_RS04470 (tssK) 938631..939968 - 1338 446631292 type VI secretion system baseplate subunit TssK
T6CP016080 TssC H1D38_RS04475 (tssC) 939984..941528 - 1545 446852935 type VI secretion system contractile sheath large subunit
T6CP016081 TssB H1D38_RS04480 (tssB) 941550..942047 - 498 446021084 type VI secretion system contractile sheath small subunit
H1D38_RS04485 942554..942745 + 192 447210540 DUF4222 domain-containing protein
H1D38_RS04490 942798..943031 - 234 446046326 hypothetical protein
H1D38_RS04495 943127..943750 - 624 447183010 hypothetical protein
H1D38_RS04500 944059..944478 - 420 485761816 hypothetical protein
H1D38_RS04505 944468..944851 - 384 446193167 hypothetical protein
H1D38_RS04510 944823..944981 - 159 446415670 hypothetical protein
H1D38_RS04515 945019..945588 - 570 446142872 inovirus Gp2 family protein
H1D38_RS04520 945792..945965 + 174 485761819 hypothetical protein
H1D38_RS04525 (hha) 946205..946417 - 213 446375476 hemolysin expression modulator Hha
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector