Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS14098  |
| T6SS type | Type i2 |
| Strain | Enterobacter cloacae strain DSM 30054 |
| Replicon (RefSeq) | chromosome (NZ_CP056776) |
| Location | 4297267..4345922 |
| Function | - |
| Comment | Deletion of the tssB1 gene in those predatory strains that were genetically tractable, which abrogated their interbacterial killing capacity. The T6SS-2-impaired mutant (ΔtssB2) of Enterobacter strain did not contribute to the antibacterial killing activity under the tested conditions. |
| References | [1] Flaugnatti N, Isaac S, Lemos Rocha LF, Stutzmann S, Rendueles O, Stoudmann C, Vesel N, Garcia-Garcera M, Buffet A, Sana TG, Rocha EPC, Blokesch M. et al (2021) Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms.. Nat Commun. 12(1):5751. [PudMed:34599171] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssE | TssG | TssJ | TssK | TssL | TssM |
| Number | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| HWQ16_RS21020 | 4292596..4293510 | + | 915 | WP_044158335.1 | OmpG family monomeric porin | ||
| HWQ16_RS21025 | 4293545..4294555 | - | 1011 | WP_013096418.1 | LacI family transcriptional regulator | ||
| HWQ16_RS21030 | 4294707..4296104 | + | 1398 | WP_013096419.1 | YcjX family protein | ||
| HWQ16_RS21035 | 4296101..4297153 | + | 1053 | WP_013096420.1 | YcjF family protein | ||
| HWQ16_RS21040 (tyrR) | 4297267..4298808 | + | 1542 | WP_013096421.1 | transcriptional regulator TyrR | ||
| HWQ16_RS21045 | 4298845..4300053 | - | 1209 | WP_013096422.1 | aldose 1-epimerase family protein | ||
| HWQ16_RS21050 (tpx) | 4300373..4300879 | - | 507 | WP_013096423.1 | thiol peroxidase | ||
| HWQ16_RS21055 (ycjG) | 4300987..4301952 | + | 966 | WP_013096424.1 | L-Ala-D/L-Glu epimerase | ||
| HWQ16_RS21060 (mpaA) | 4301943..4302656 | - | 714 | WP_013096425.1 | murein tripeptide amidase MpaA | ||
| HWQ16_RS21065 | 4302839..4304455 | + | 1617 | WP_013096426.1 | peptide ABC transporter substrate-binding protein | ||
| T6CP210289 | TssB | HWQ16_RS21070 (tssB) | 4305040..4305531 | + | 492 | WP_013096428.1 | type VI secretion system contractile sheath small subunit |
| T6CP210290 | TssC | HWQ16_RS21075 (tssC) | 4305573..4307114 | + | 1542 | WP_044159362.1 | type VI secretion system contractile sheath large subunit |
| T6CP210291 | TssK | HWQ16_RS21080 (tssK) | 4307132..4308472 | + | 1341 | WP_013096430.1 | type VI secretion system baseplate subunit TssK |
| T6CP210292 | TssL | HWQ16_RS21085 | 4308469..4309161 | + | 693 | WP_013096431.1 | type VI secretion system protein TssL, short form |
| HWQ16_RS21090 | 4309158..4310630 | + | 1473 | pseudogene | OmpA family protein | ||
| HWQ16_RS21095 (tssH) | 4310657..4312915 | + | 2259 | pseudogene | type VI secretion system ATPase TssH | ||
| HWQ16_RS21100 (vgrG) | 4312912..4315704 | + | 2793 | pseudogene | type VI secretion system tip protein VgrG | ||
| HWQ16_RS21105 | 4315701..4316132 | + | 432 | WP_077681880.1 | DUF4431 domain-containing protein | ||
| HWQ16_RS21110 | 4316943..4317983 | + | 1041 | WP_109455520.1 | TIGR02594 family protein | ||
| HWQ16_RS21115 | 4318067..4318495 | + | 429 | WP_044159353.1 | hypothetical protein | ||
| HWQ16_RS21120 | 4318591..4318824 | + | 234 | WP_013096436.1 | hypothetical protein | ||
| HWQ16_RS21125 | 4319238..4320446 | + | 1209 | WP_013096437.1 | hypothetical protein | ||
| T6CP210294 | TssM | HWQ16_RS21130 | 4320443..4323916 | + | 3474 | WP_013096438.1 | type VI secretion system protein ImpL |
| T6CP210295 | TssA | HWQ16_RS21135 (tssA) | 4323916..4325514 | + | 1599 | WP_013096439.1 | type VI secretion system protein TssA |
| HWQ16_RS21140 | 4325548..4325709 | + | 162 | pseudogene | PAAR domain-containing protein | ||
| HWQ16_RS21145 | 4325741..4326709 | + | 969 | WP_013087110.1 | IS5-like element IS903B family transposase | ||
| HWQ16_RS21150 (tssF) | 4326737..4328233 | + | 1497 | pseudogene | type VI secretion system baseplate subunit TssF | ||
| T6CP210296 | TssG | HWQ16_RS21155 (tssG) | 4328197..4329282 | + | 1086 | WP_013096442.1 | type VI secretion system baseplate subunit TssG |
| T6CP210297 | TssJ | HWQ16_RS21160 (tssJ) | 4329260..4329805 | + | 546 | WP_013096443.1 | type VI secretion system lipoprotein TssJ |
| T6CP210298 | TssE | HWQ16_RS21165 (tssE) | 4329805..4330260 | + | 456 | WP_013096444.1 | type VI secretion system baseplate subunit TssE |
| T6CP210299 | TssA | HWQ16_RS21170 | 4330284..4331600 | + | 1317 | WP_013096445.1 | type VI secretion system protein VasL |
| HWQ16_RS21175 | 4331719..4332198 | + | 480 | WP_013096446.1 | hypothetical protein | ||
| HWQ16_RS21180 | 4332233..4332796 | - | 564 | WP_013096447.1 | DJ-1/PfpI family protein | ||
| HWQ16_RS21185 (ecr) | 4333014..4333154 | - | 141 | WP_013096448.1 | Ecr family regulatory small membrane protein | ||
| HWQ16_RS21190 | 4333229..4334461 | - | 1233 | WP_013096449.1 | sensor domain-containing diguanylate cyclase | ||
| HWQ16_RS21195 | 4334545..4336683 | - | 2139 | WP_044158464.1 | EAL domain-containing protein | ||
| HWQ16_RS21200 | 4337140..4338294 | + | 1155 | WP_065242442.1 | chemoreceptor protein | ||
| HWQ16_RS21205 (zntB) | 4338414..4339397 | + | 984 | WP_013096452.1 | zinc transporter ZntB | ||
| HWQ16_RS21210 (dbpA) | 4339882..4341255 | + | 1374 | WP_013096453.1 | ATP-dependent RNA helicase DbpA | ||
| HWQ16_RS21215 (ttcA) | 4341302..4342237 | - | 936 | WP_013096454.1 | tRNA 2-thiocytidine(32) synthetase TtcA | ||
| HWQ16_RS21220 | 4342615..4342833 | - | 219 | WP_032618552.1 | YgdI/YgdR family lipoprotein | ||
| HWQ16_RS21225 (msbA) | 4344165..4345922 | + | 1758 | WP_044158462.1 | lipid A ABC transporter ATP-binding protein/permease MsbA | ||
| HWQ16_RS21230 | 4346010..4346390 | + | 381 | WP_044158460.1 | DUF1294 domain-containing protein | ||
| HWQ16_RS21235 | 4346489..4346938 | + | 450 | WP_013096458.1 | hypothetical protein | ||
| HWQ16_RS21240 | 4346978..4347175 | + | 198 | WP_013096459.1 | hypothetical protein | ||
| HWQ16_RS21245 | 4347179..4347604 | + | 426 | WP_044158456.1 | GFA family protein | ||
| HWQ16_RS21250 | 4348083..4349192 | - | 1110 | WP_013096462.1 | porin OmpS2 |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|