Detailed information of regulator
Summary
| Regulator ID | REG0203  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00344 (Type i2) |
| Strain | Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044 |
| Replicon | chromosome |
| Sequence | Protein sequence (136 a.a.); Nucleotide sequence (408 bp) |
| Description | H-NS specifically binds to TssD region in K.pneumoniae NTUH-K2044 T6SS locus I.; H-NS specifically binds to hcp region in K.pneumoniae NTUH-K2044 T6SS locus I and represses hcp expression and secretion. |
| Reference |
External database links
| Locus tag (Gene) | KP1_3314 (hns) |
| Coordinates (Strand) | 3140175..3140582 (+) |
| NCBI ID | 238895262 |
| RefSeq | NC_012731 |
| Uniprot ID | UPI00015769B9 |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| KP1_2392 (TssB) promotor | ↑ | caaacattcctttggtgcattatttattgaatttatcattgacggctgttacacattttaaatattgatattcatggtgcttttattttcgtggtgaaagggggtgtttatgaataaatataattattaggtggttaagttgatcgttaatccatttttgctttatcatcgtcacgtttcacatcaatatatcttcaataattatttcttaaggaaaggaactgctatggctgatacgttc |
| KP1_2392 (hcp) | ↑ | attccatggcaattcctgtttatctttggctgaaagacgacggcggcgcggacattaaagggtctgtcgacgttcaggatcgtgaaggcagtatcgaagtggtggcccaggagcataacctgtacatcccgaccgataacaacaccggcaaactgaccggcacgcgtattcatacgccgttcctgttcaccaaggagatcgactcctccagcccctacctgtacaaggcggtgaccaccggccagaccctgaaatccgccgagttcaagtggtaccgaatcaacgatgccggccaggaagtggagtattt |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Histone_HNS | PF00816.23 | 1.2e-18 | 33..127 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-135 | MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEENAAAAEIEERTRKLQQYREMLIADGIDPNELLSTMAAVKAGTKTKRAARPAKYSYVDENGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLI |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9966 | - | - |
Protein sequence of regulator: 136 a.a. .
>REG0203 NC_012731:3140175-3140582 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEENAAAAEIEERTRKLQQYREMLIADGIDPNELLSTMA
AVKAGTKTKRAARPAKYSYVDENGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLI*
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEENAAAAEIEERTRKLQQYREMLIADGIDPNELLSTMA
AVKAGTKTKRAARPAKYSYVDENGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLI*
Nucleotide sequence of regulator: 408 bp .
>REG0203 NC_012731:3140175-3140582 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGAGCGAAGCACTTAAAATTTTGAACAACATCCGTACTCTTCGTGCGCAGGCAAGAGAATGCACCCTGGAAACGCTCGA
AGAAATGCTGGAAAAATTAGAAGTCGTCGTTAACGAGCGCCGTGAAGAAGAAAACGCTGCCGCGGCAGAAATTGAAGAGC
GTACGCGTAAGCTGCAGCAATACCGTGAAATGCTGATTGCTGACGGTATTGATCCGAATGAACTGCTGAGCACCATGGCT
GCCGTTAAAGCCGGTACCAAAACCAAGCGTGCTGCACGTCCGGCTAAATATAGCTATGTTGACGAAAATGGTGAAACCAA
AACCTGGACCGGTCAGGGCCGTACCCCGGCTGTGATCAAAAAAGCAATGGATGAGCAAGGTAAATCACTGGACGATTTCC
TGATCTAA
ATGAGCGAAGCACTTAAAATTTTGAACAACATCCGTACTCTTCGTGCGCAGGCAAGAGAATGCACCCTGGAAACGCTCGA
AGAAATGCTGGAAAAATTAGAAGTCGTCGTTAACGAGCGCCGTGAAGAAGAAAACGCTGCCGCGGCAGAAATTGAAGAGC
GTACGCGTAAGCTGCAGCAATACCGTGAAATGCTGATTGCTGACGGTATTGATCCGAATGAACTGCTGAGCACCATGGCT
GCCGTTAAAGCCGGTACCAAAACCAAGCGTGCTGCACGTCCGGCTAAATATAGCTATGTTGACGAAAATGGTGAAACCAA
AACCTGGACCGGTCAGGGCCGTACCCCGGCTGTGATCAAAAAAGCAATGGATGAGCAAGGTAAATCACTGGACGATTTCC
TGATCTAA