Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000045  |
| Strain | Escherichia coli strain C600 |
| Replicon | chromosome |
| Sequence | Protein sequence (163 a.a.); Nucleotide sequence (489 bp) |
| Response to T6SS attack | protective |
| Known or putative function | serine protease inhibitor |
| Description | Survival of the Δeco mutant could be largely restored upon introduction of a plasmid encoding the wild-type eco+ gene, and the Δeco mutant could be rescued by the exogenously added serine protease inhibitor Pefabloc |
| Reference |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | DK885_RS08170 (eco) |
| Coordinates (Strand) | 1618127..1618615 (-) |
| NCBI ID | 446771953 |
| RefSeq | NZ_CP031214 |
| Uniprot ID | M9FUA1_ECOLX, S1IGA1_ECOLX, ECOT_ECODH, E3PDR1_ECOH1, ECOT_ECOHS, A0A0E1SZS0_ECOLX, A0A140N645_ECOBD, ECOT_ECOLC, A0A037Y6Z4_ECOLX, ECOT_ECO8A, ECOT_ECOBW, A0A0E1LZA0_ECOLX, ECOT_ECOLI, I2WMY3_ECOLX |
| KEGG ID | ecd:ECDH10B_2366, ebd:ECBD_1450, elh:ETEC_2343, ecx:EcHS_A2347, ecj:JW2197, ebw:BWG_1983, ecr:ECIAI1_2293, eco:b2209, ecl:EcolC_1441 |
| PDB ID | 1ECY, 1XXD, 1EZU, 1SLX, 1ID5, 1IFG, 1P0S, 7CBK, 1XXF, 1AZZ, 1FI8, 4IW4, 1ECZ, 1EZS, 1SLW, 1XX9, 4NIY, 1SLV, 1SLU, 1N8O |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Ecotin | PF03974.15 | 2.6e-50 | 30..152 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-162 | MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVVR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9986 | pos: 20-21. AWA-AE. | MKTILPAVLFAAFATTSAWA |
Protein sequence: 163 a.a. .
>RSF000045 NZ_CP031214:c1618615-1618127 [Escherichia coli]
MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLE
NKTLEGWGYDYYVFDKVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
VR*
MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLE
NKTLEGWGYDYYVFDKVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
VR*
Nucleotide sequence: 489 bp .
>RSF000045 NZ_CP031214:c1618615-1618127 [Escherichia coli]
ATGAAGACCATTCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACT
GGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGATTCAGTTAACCCCGCAAGAAGATGAAT
CTACCCTGAAAGTAGAACTGTTAATCGGTCAGACGCTGGAAGTCGATTGCAATTTGCATCGTCTCGGCGGGAAGCTGGAA
AACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTG
CCCGGATGGCAAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGC
CGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTA
GTTCGCTAA
ATGAAGACCATTCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACT
GGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGATTCAGTTAACCCCGCAAGAAGATGAAT
CTACCCTGAAAGTAGAACTGTTAATCGGTCAGACGCTGGAAGTCGATTGCAATTTGCATCGTCTCGGCGGGAAGCTGGAA
AACAAAACGCTGGAAGGCTGGGGCTATGATTATTATGTCTTTGATAAAGTCAGTTCCCCGGTTTCAACGATGATGGCCTG
CCCGGATGGCAAGAAAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGC
CGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTA
GTTCGCTAA