Detailed information of regulator
Summary
| Regulator ID | REG0342  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS01214 (Type Unknown) |
| Strain | Klebsiella pneumoniae strain Kp52.145 |
| Replicon | chromosome |
| Sequence | Protein sequence (136 a.a.); Nucleotide sequence (408 bp) |
| Description | The expression of tssB::lucFF was significantly higher in the h-ns, mgrB and rcsB mutant backgrounds than in the wild-type one and co-incubation of the h-ns, mgrB and rcsB mutants with E. coli in LB resulted in a significant reduction in E. coli survival. |
| Reference |
External database links
| Locus tag (Gene) | BN49_RS18480 (h-ns) |
| Coordinates (Strand) | 3427636..3428043 (+) |
| NCBI ID | 488999426 |
| RefSeq | NZ_FO834906 |
| Uniprot ID | A0A0H3GUZ5_KLEPH, J2X6T6_KLEPN, V0AJS6_KLEPN, A0A0E1CD91_KLEPN, W8UIU7_KLEPN, A0A1C1F5G4_9ENTR, A0A1F2M8X2_9ENTR, A0A223UAK6_9ENTR, A0A2S0VKE8_9ENTR, A0A087FPY4_KLEVA, A6TAK8_KLEP7, B5XQ96_KLEP3, A0A1Y0PYQ6_KLEPN, R4YAU9_KLEPR |
| KEGG ID | kqu:AVR78_20130, kpe:KPK_2107, kpv:KPNIH29_15970, kpm:KPHS_31980, kps:KPNJ2_02205, kpw:KPNIH30_16255, kpr:KPR_3120, ebu:CUC76_25475, kpy:KPNIH31_15440, kpn:KPN_02201, kvd:KR75_24365, kpg:KPNIH32_16150, kpk:A593_00580, kvq:SP68_04640, kpa:KPNJ1_02245 |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| TssB transcription | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Histone_HNS | PF00816.23 | 1.2e-18 | 33..127 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-135 | MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEENAAAAEIEERTRKLQQYREMLIADGIDPNELLSTMAAVKAGTKTKRAARPAKYSYVDENGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLI |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9966 | - | - |
Protein sequence of regulator: 136 a.a. .
>REG0342 NZ_FO834906:3427636-3428043 [Klebsiella pneumoniae]
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEENAAAAEIEERTRKLQQYREMLIADGIDPNELLSTMA
AVKAGTKTKRAARPAKYSYVDENGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLI*
MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEENAAAAEIEERTRKLQQYREMLIADGIDPNELLSTMA
AVKAGTKTKRAARPAKYSYVDENGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLI*
Nucleotide sequence of regulator: 408 bp .
>REG0342 NZ_FO834906:3427636-3428043 [Klebsiella pneumoniae]
ATGAGCGAAGCACTTAAAATTTTGAACAACATCCGTACTCTTCGTGCGCAGGCAAGAGAATGCACCCTGGAAACGCTCGA
AGAAATGCTGGAAAAATTAGAAGTCGTCGTTAACGAGCGCCGTGAAGAAGAAAACGCTGCCGCGGCAGAAATTGAAGAGC
GTACGCGTAAGCTGCAGCAATACCGTGAAATGCTGATTGCTGACGGTATTGATCCGAATGAACTGCTGAGCACCATGGCT
GCCGTTAAAGCCGGTACCAAAACCAAGCGTGCTGCACGTCCGGCTAAATATAGCTATGTTGACGAAAACGGTGAAACCAA
AACCTGGACCGGTCAGGGCCGTACCCCGGCTGTGATCAAAAAAGCAATGGATGAGCAAGGTAAATCACTGGACGATTTCC
TGATCTAA
ATGAGCGAAGCACTTAAAATTTTGAACAACATCCGTACTCTTCGTGCGCAGGCAAGAGAATGCACCCTGGAAACGCTCGA
AGAAATGCTGGAAAAATTAGAAGTCGTCGTTAACGAGCGCCGTGAAGAAGAAAACGCTGCCGCGGCAGAAATTGAAGAGC
GTACGCGTAAGCTGCAGCAATACCGTGAAATGCTGATTGCTGACGGTATTGATCCGAATGAACTGCTGAGCACCATGGCT
GCCGTTAAAGCCGGTACCAAAACCAAGCGTGCTGCACGTCCGGCTAAATATAGCTATGTTGACGAAAACGGTGAAACCAA
AACCTGGACCGGTCAGGGCCGTACCCCGGCTGTGATCAAAAAAGCAATGGATGAGCAAGGTAAATCACTGGACGATTTCC
TGATCTAA