Detailed information of Recipient susceptibility factor

Summary

ID	RSF000035
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (208 a.a.); Nucleotide sequence (624 bp)
Response to T6SS attack	sensitive
Known or putative function	serine protease
Description	Five mutants, clpP, gltA, ydhS, ydaE, and cbpA showed lower susceptibility to A. tumefaciens T6SS attack than that of BW25113 wild type.
Reference	[1] Lin HH, Yu M, Sriramoju MK, et al. A High-Throughput Interbacterial Competition Screen Identifies ClpAP in Enhancing Recipient Susceptibility to Type VI Secretion System-Mediated Attack by Agrobacterium tumefaciens. Front Microbiol. 2020 Feb 5;10:3077. doi: 10.3389/fmicb.2019.03077. eCollection 2019. PMID: 32117077

Experimental investigation has been performed.

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External database links

Locus tag (Gene)	BW25113_RS02265 (clpP)
Coordinates (Strand)	452133..452756 (+)
NCBI ID	446044398
RefSeq	NZ_CP009273
Uniprot ID	A0A1Z3UU14_ECOLX, M9HD84_ECOLX, A0A0E0Y6A5_ECO1C, K4Y0L2_ECOLX, S1PK16_ECOLX, L3BTR8_ECOLX, S1EW18_ECOLX, L3QH23_ECOLX, S1I5L7_ECOLX, L4JED2_ECOLX, V0AH82_ECOLX, A0A0E2L9M1_ECOU3, A0A080JG42_ECOLX, A0A029IB76_ECOLX, A0A0A0FC08_ECOLX, CLPP_ECOL6, D3GV67_ECO44, CLPP_SHIDS, E3PFQ3_ECOH1, CLPP_ECO24, CLPP_ECOHS, A0A0E1SUV7_ECOLX, CLPP_ECOL5, CLPP_ECOUT, CLPP_ECOK1, B6HZP4_ECOSE, B1LJJ4_ECOSM, A0A140NEW8_ECOBD, J7RD56_ECOLX, A0A0H3PR91_ECO5C, CLPP_ECOLC, D6J7D7_ECOLX, A0A2S7SNY8_ESCFE, E0J0J9_ECOLW, A0A169QWL1_KLEOX, B7UJR0_ECO27, B7MD96_ECO45, B7LME2_ESCF3, B7L674_ECO55, B7N8Z1_ECOLU, A0A0H3MEA1_ECO7I, B7MQF3_ECO81, A0A1Q8MKU4_SHIBO, A0A1Q8NHH5_SHIDY, CLPP_SHIFL, A0A0I2X2B6_SHISO, A0A1X3JLI8_ECOLX, A0A1X3L7N0_ECOLX, A0A1X3K7X8_ECOLX, F4VAM3_ECOLX, A0A1X3KPW7_ECOLX, F4SJD3_ECOLX, A0A1X3IAW6_ECOLX, A0A0K9TA59_ECOLX, F4SU63_ECOLX, F4TAV2_ECOLX, A0A1X3LNB1_ECOLX, A0A1X3LUB4_ECOLX, A0A1X3J6K0_ECOLX, E9XPL3_ECOLX, E1IPF1_ECOLX, D8E114_ECOLX, A0A0H3EFS7_ECO8N, D3QJK8_ECOCB, A0A0E1LU99_ECOLX, D7XK90_ECOLX, D7Y330_ECOM1, I4SYK4_ECOLX, CLPP_ECOLI, CLPP_ECO57, I2SZB4_ECOLX, I2UQT2_ECOLX, I2WT21_ECOLX, A0A192CHZ0_ECOLX, W8ZN51_ECOLX
KEGG ID	elh:ETEC_0490, ecq:ECED1_0461, eci:UTI89_C0465, esl:O3K_19305, elo:EC042_0475, eum:ECUMN_0477, eln:NRG857_02065, ecy:ECSE_0463, ecw:EcE24377A_0473, efe:EFER_2580, ece:Z0542, sfx:S0388, ecg:E2348C_0372, ecp:ECP_0498, ecx:EcHS_A0514, ecl:EcolC_3195, ecc:c0553, ect:ECIAI39_0236, ecj:JW0427, ecs:ECs0491, eck:EC55989_0451, eco:b0437, elw:ECW_m0509, ebd:ECBD_3220, ecv:APECO1_1574, sdy:SDY_0295, eok:G2583_0549, ecos:EC958_0576, sfl:SF0382, ecz:ECS88_0434, ecm:EcSMS35_0480
PDB ID	6NB1, 2FZS, 6PO3, 6UQE, 6WR2, 1YG8, 6W1Z, 6POD, 6WRF, 1YG6, 6WSG, 3MT6, 6PO1, 1TYF, 6POS, 6UQO, 6W21, 3HLN, 6PPE, 6W20

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Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
CLP_protease	PF00574.25	1.7e-88	26..205

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Transmembrane helices

• Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-207	MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIFLTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVSTICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVKGRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN

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Signal peptides

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9949	-	-

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  Protein sequence: 208 a.a.    .Download


  Nucleotide sequence: 624 bp    .Download