SecReT6, T6SS database

Detailed information of Recipient susceptibility factor

Summary

ID	RSF000031
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (465 a.a.); Nucleotide sequence (1395 bp)
Response to T6SS attack	protective
Known or putative function	RcsB regulon response envelope stress
Description	Deletion of the wcaJ gene, resulting in loss of colanic acid while also abolishing potentially toxic intermediates during its synthesis, also demonstrated reduced survival compared with the WT with a nearly significant P value of 0.0527.
Reference	[1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [3] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

External database links

Locus tag (Gene)	BW25113_RS10690 (wcaJ)
Coordinates (Strand)	2113641..2115035 (-)
NCBI ID	446105252
RefSeq	NZ_CP009273
Uniprot ID	A0A0E1T1A7_ECOLX, A0A1L6AY14_ECOLX, WCAJ_ECOLI
KEGG ID	eco:b2047, ecj:JW2032
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Bac_transf	PF02397.18	3.6e-67	274..456
CoA_binding_3	PF13727.8	2.8e-41	65..238

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-11	MTNLKKRERAK
Transmembrane helix	12-34	TNASLISMVQRFSDITIMFAGLW
Outside	35-43	LVCEVSGLS
Transmembrane helix	44-66	FLYMHLLVALITLVVFQMLGGIT
Inside	67-78	DFYRSWRGVRAA
Transmembrane helix	79-101	TEFALLLQNWTLSVIFSAGLVAF
Outside	102-110	NNDFDTQLK
Transmembrane helix	111-130	IWLAWYALTSIGLVVCRSCI
Inside	131-278	RIGAGWLRNHGYNKRMVAVAGDLAAGQMLMESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGARVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRAED
Transmembrane helix	279-300	IVLATLILLLISPVLCCIALAV
Outside	301-464	KLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9972	-	-

Protein sequence: 465 a.a. .

                                    >RSF000031 NZ_CP009273:c2115035-2113641 [Escherichia coli BW25113]
MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATE
FALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYALTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGARVKKLVHQLADTTCSVLLIPDV
FTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRAEDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGK
PIKVWKFRSMKVMENDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYML
RHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY*

                                

Nucleotide sequence: 1395 bp .

                                    >RSF000031 NZ_CP009273:c2115035-2113641 [Escherichia coli BW25113]
ATGACAAATCTAAAAAAGCGCGAGCGAGCGAAAACCAATGCATCGTTAATCTCTATGGTGCAACGCTTTTCAGATATCAC
CATCATGTTTGCCGGACTATGGCTGGTTTGCGAAGTCAGCGGACTGTCATTCCTCTACATGCACCTGTTGGTGGCGCTGA
TTACGCTGGTGGTGTTCCAGATGCTGGGCGGCATCACCGATTTTTATCGCTCATGGCGCGGTGTTCGGGCAGCGACAGAA
TTTGCCCTGTTGCTACAAAACTGGACCTTAAGCGTGATTTTCAGCGCCGGACTGGTGGCGTTCAACAATGATTTCGACAC
GCAACTGAAAATCTGGCTGGCGTGGTATGCGCTGACCAGCATCGGACTGGTGGTTTGCCGTTCGTGTATTCGCATTGGGG
CGGGCTGGCTGCGTAATCATGGCTATAACAAGCGCATGGTCGCGGTGGCGGGGGATTTAGCCGCCGGGCAAATGCTGATG
GAGAGCTTCCGTAACCAGCCGTGGTTAGGGTTTGAAGTGGTGGGCGTTTACCACGACCCGAAACCGGGCGGCGTTTCTAA
CGACTGGGCGGGTAACCTGCAACAGCTGGTCGAGGACGCGAAAGCGGGCAAGATTCATAACGTCTATATCGCGATGCAAA
TGTGCGACGGCGCGCGAGTGAAAAAACTGGTCCATCAACTGGCGGACACCACCTGTTCGGTGCTGCTGATCCCCGACGTC
TTTACCTTCAACATTCTCCATTCACGCCTCGAAGAGATGAACGGCGTACCGGTGGTGCCGCTTTACGACACGCCGCTTTC
CGGGGTTAACCGCCTGCTCAAACGTGCGGAAGACATTGTGCTGGCGACGCTTATTCTGCTGCTGATCTCCCCGGTGCTGT
GCTGTATTGCGCTGGCGGTGAAACTCAGTTCACCAGGGCCGGTTATTTTCCGCCAGACTCGCTACGGCATGGATGGCAAG
CCGATCAAAGTGTGGAAGTTCCGTTCCATGAAAGTGATGGAGAACGACAAAGTGGTGACCCAGGCGACGCAGAACGATCC
GCGCGTCACCAAAGTGGGGAACTTTCTGCGCCGTACCTCGCTGGATGAATTGCCGCAGTTTATCAATGTGCTGACCGGGG
GGATGTCGATTGTCGGTCCACGTCCGCACGCAGTAGCGCATAACGAACAGTATCGACAGCTCATTGAAGGCTACATGCTG
CGCCATAAGGTGAAACCGGGCATTACCGGCTGGGCGCAGATTAACGGCTGGCGCGGCGAAACCGACACGCTGGAGAAAAT
GGAAAAACGCGTCGAGTTCGACCTTGAGTACATCCGCGAATGGAGCGTCTGGTTCGATATCAAAATCGTTTTCCTGACGG
TGTTCAAAGGTTTCGTTAACAAAGCGGCATATTGA

                                